Sachin S. Salunkhe

Development of Lipid Based Nanoparticulate Drug Delivery Systems and Drug Carrier Complexes for Delivery to Brain

Many neurotherapeutics are unsuccessful in treating CNS disorders because they cannot be effectively drug delivered. Drug delivery to the brain is a challenge even though there is relatively high blood flow. There are two physiological barriers likes blood-brain barrier and blood-cerebrospinal fluid barrier which separates the brain from its blood supply controlling the transport of compounds. Many of the brain or CNS associated diseases remain untreated by effective therapies. This is not because there is a lack of candidate drugs but due to the inability of many therapeutic molecules to cross the BBB, the BCSFB or other specialized CNS barriers to reach the specific areas of brain. Hence there is a need in the modern approaches and present insights into using ligand conjugation and nanotechnology to target the BBB via different transport pathways and mechanisms. The field of novel drug delivery system has fully emerged and came into existence as an ideal approach of drug targeting and delivery to brain. The new approaches of drug delivery to brain help in successful transporting drugs across the BBB.

Lung delivery of nanoliposomal salbutamol sulphate dry powder inhalation for facilitated asthma therapy

Abstract The motive behind present work was to discover a solution for overcoming the problems allied with a deprived oral bioavailability of salbutamol sulfate (SS) due to its first pass hepatic metabolism, shorter half-life, and systemic toxicity at high doses. Pulmonary delivery provides an alternative route of administration to avoid hepatic metabolism of SS, moreover facilitated diffusion and prolonged retention can be achieved by incorporation into liposomes. Liposomes were prepared by thin film hydration technique using 32 full factorial design and formulation was optimized based on the vesicle size and percent drug entrapment (PDE) of liposomes. Optimized liposomal formulation exhibited an average size of about 167.2 ± 0.170 nm, with 80.68 ± 0.74% drug entrapment, and 9.74 ± 1.10 mV zeta potential. The liposomal dispersion was then spray dried and further characterized for in-vitro aerosol performance using Andersen Cascade Impactor. Optimized liposomal formulation revealed prolonged in-vitro drug release of more than 90% up to 14 h following Higuchi’s controlled release model. Thus, the proposed new-fangled liposomal formulation would be a propitious alternative to conventional therapy for efficient and methodical treatment of asthma and alike respiratory ailments.

COMPARATIVE EFFICIENCY OF FORMULATION TECHNIQUES FOR DEVELOPMENT OF SALBUTAMOL SULPHATE LOADED LIPOSOMES

Present study investigate d most efficient technique for the develop ment of salbutamol sulphate loaded liposom es . Salbutamol sulphate is a selective β 2 adrenoceptor agonist widely used in the treatment of bronchial asthma, chronic bronchitis and emphysema. Our work is mainly focused on in vitro st udies of liposomal formulation encapsulated with salbutamol sulphate which may have high drug entrapment, sustained drug release and effective vesicle size. Drug loaded liposome s were prepared by ethanol injectio n and thin film hydration techniques using s oyaphosphatidyl choline and cholesterol in various molar ratios . Liposomes were evaluated for vesicle size, entrapment efficiency, transmission electron microscopy, zeta potential and drug release parameters. The particle size of drug loaded liposomes prep ared by ethanol injection technique was 423 ‐ 527 nm whereas it was 160 - 174.2 nm when prepared by thin film hydration technique. Z eta potential of liposomes prepared by thin film hydration technique was enough to stabilize over six month s . Optimized batches of s albutamolsulphate loaded liposomal formulations prepared by ethanol injection and thin film hydration technique have shown drug release as 94.59 % and 88.03 %, respectively . On the basis of observed a verage particle size, percent drug entrapment and dr ug release profiles, thin film hydration technique was emerged as superior technique over ethanol injection technique.