Sachio Hisanaga

Unexpectedly low lecithin/sphingomyelin ratio associated with fetal diaphragmatic hernia

Devore GR, Donnerstein RL, Kleinman CS, Platt LD, Hobbins JC. Fetal echocardiography. 1. Normal anatomy as determined by real-time-directed M-mode ultrasound. AMJ OBSTET GYNECOL 1982;144:249-60. Berkowitz RL, Glickman MG, Smith GJW, Siegel NJ, Weiss RM, Mahoney MJ, Hobbins JC. Fetal urinary tract obstruction: what is the role of surgical intervention in utero? AM J OBSTET GYNECOL 1982;144:367-75.

A new method for measurement of surfactant specific apoprotein in amniotic fluid

A simple and rapid method for the quantitative measurement of surfactant specific apoprotein concentration in amniotic fluid was developed using the measurement of immunological reactions by the nephelometric technique. Use of this method made it possible to measure 0.5–8.0 μg apoproteins per ml within approximately 70 min. Surfactant specific apoproteins in 54 samples of amniotic fluid were measured using the method. The surfactant specific apoprotein concentration in amniotic fluid increased from 1.03 ± 0.51 μg/ml (mean ± S.D.) at 26–30 weeks of gestation to 4.45 ± 2.08 μg/ml at 36 weeks of gestation or more. Among premature infants, who were delivered within 24 h after amniocentesis, surfactant specific apoprotein concentration was less than 1.5 μg/ml in three infants with respiratory distress syndrome (RDS), and more than 2.1 μg/ml in six without RDS. The results indicated that the quantitative measurement of surfactant specific apoprotein in amniotic fluid is effective in predicting the fetal lung maturity, and that simplicity and rapidity make our method useful for clinical application.

Fraction of urinary protein in human pregnancy and puperperium by SDS-polyacrylamide gel electrophoresis.

正常妊娠における尿中蛋白組成の変化を分析するために今回の研究を施行した.15例の正常妊婦の妊娠中の尿中蛋白組成の変化をSDS-ポリアクリルアミドゲル電気泳動法 (以下SDS-PAGEと略) により分析した.その結果, 妊娠初期には1本あるいは2本 (分子量71×103, 94×103) の帯が認められ, 妊娠経過に伴い帯の数が増加し, 妊娠後期には7本の帯が認められた. しかし産褥1ヶ月では, 帯の数は再び2本になり, 妊娠初期と同じ像を呈した.今回の結果より, 妊娠中の尿中蛋白の分析にSDS-PAGEが有用であることがわかった.