Sajid Masood

Mechanism of Potassium Solubilization in the Agricultural Soils by the Help of Soil Microorganisms

Potassium (K) is an essential macronutrient, largely required for normal functioning of plants; however, its solubilization is an important detriment for limiting agricultural productivity. This happens mainly because of insoluble forms of K, i.e. silicate minerals are present in the earth’s crust. These minerals gradually dissolute K through different weathering processes. However, the use of certain microbes can assist the solubilization of K in addition to physical and chemical weathering of K minerals. These microorganisms, particularly bacteria, convert insoluble form of K to soluble forms, viz. acidification, chelation and exchange reactions. In this chapter, efforts are made to describe the possible ways of K release by the microbes. In addition, potential of different microbial species is summarized. Moreover, suggestions are provided for the sustainable plant production. In this way, the substantial decrease in the cost of commercial fertilizers could be expected.

Arsenic in groundwater and its health risk assessment in drinking water of Mailsi, Punjab, Pakistan

ABSTRACT The present study was aimed at assessing drinking water quality regarding arsenic (As) and its impact on health from Mailsi (Punjab), Pakistan. Forty-four groundwater samples were collected from two sites, Sargana and Mailsi. Arsenic and other cations were determined by atomic absorption spectrophotometer, whereas the anions were determined either through titration or spectrophotometer. The results revealed that dominant anions were HCO3− and Cl−, Ca+2 was the dominant cation, and overall water chemistry of the area was CaMgHCO3− type. Arsenic concentrations were high, ranging from 11 to 828 µg/L that crossed the World Health Organization permissible limits. Likewise, higher SO4−2 concentrations ranging from 247 to 1053 mg/L were observed. The health risk index was higher in the Sargana site, which employed the differences in terms of higher Average Daily Dose, Hazard Quotient, and Carcinogenic Risk of arsenic, which is unsuitable for drinking purposes. The area seems to be at high risk due to arsenic pollution and wells have never been tested for arsenic concentrations earlier; therefore, necessary measures should be taken to test the wells with respect to arsenic.

Chromium toxicity tolerance of Solanum nigrum L. and Parthenium hysterophorus L. plants with reference to ion pattern, antioxidation activity and root exudation.

Chromium (Cr), being a highly toxic metal, adversely affects the mineral uptake and metabolic processes in plants when present in excess. The current study was aimed at investigating the Cr accumulation in various plant tissues and its relation to the antioxidation activity and root exudation. Plants were grown in soil spiked with different concentrations of Cr for three weeks in pots and analysed for different growth, antioxidants and ion attributes. Furthermore, plants treated with different concentrations of Cr in pots were shifted to rhizobox-like system for 48h and organic acids were monitored in the mucilage dissolved from the plant root surface, mirroring rhizospheric solution. The results revealed that the Cr application at 1mM increased the shoot fresh and dry weight and root dry weight of Solanum nigrum, whereas the opposite was observed for Parthenium hysterophorus when compared with lower levels of Cr (0.5mM) or control treatment. In both plant species, Cr and Cl concentrations were increased while Ca, Mg and K concentrations in root, shoot and root exudates were decreased with increasing levels of Cr. Higher levels of Cr treatments enhanced the activities of SOD, POD and proline content in leaves of S. nigrum, whereas lower levels of Cr treatment were found to have stimulatory effects in P. hysterophorus. P. hysterophorus exhibited highest exudation of organic acid contents. With increasing levels of Cr treatments, citric acid concentration in root exudates increased by 35% and 44% in S. nigrum, whereas 20% and 76% in P. hysterophorus. Cr toxicity was responsible for the shoot growth reduction of S. nigrum and P. hysterophorus, however, shoot growth response was different at different levels of applied Cr. Consequently, Cr stress negatively altered the plant physiology and biochemistry. However, the enhanced antioxidant production, Cl uptake and root exudation are the physiological and biochemical indicators for the plant adaptations in biotic systems polluted with Cr.

Phyto-Extraction of Nickel by Linum usitatissimum in Association with Glomus intraradices

Plants show enhanced phytoremediation of heavy metal contaminated soils particularly in response to fungal inoculation. Present study was conducted to find out the influence of Nickel (Ni) toxicity on plant biomass, growth, chlorophyll content, proline production and metal accumulation by L. usitatissimum (flax) in the presence of Glomus intraradices. Flax seedlings of both inoculated with G. intraradices and non-inoculated were exposed to different concentrations i.e., 250, 350 and 500 ppm of Ni at different time intervals. Analysis of physiological parameters revealed that Ni depressed the growth and photosynthetic activity of plants. However, the inoculation of plants with arbuscular mycorrhizae (G. intraradices) partially helped in the alleviation of Ni toxicity as indicated by improved plant growth under Ni stress. Ni uptake of non- mycorrhizal flax plants was increased by 98% as compared to control conditions whereas inoculated plants showed 19% more uptake when compared with the non-inoculated plants. Mycorrhizal plants exhibited increasing capacity to remediate contaminated soils along with improved growth. Thus, AM assisted phytoremediation helps in the accumulation of Ni in plants to reclaim Ni toxic soils. Based on our findings, it can be concluded that the role of flax plants and mycorrhizal fungi is extremely important in phytoremediation.

Comparison of various potting media for their influence on nutrients supply, germination, and growth of muskmelon (cucumis melo L.) seedlings

ABSTRACT Peat moss is used as potting mix for growing muskmelon (Cucumis melo L.) seedlings. However, it is not economical for most of the farmers in developing countries. The objectives of the present study were to compare various sources of composts as substitutes for peat moss, evaluate their physicochemical properties, and their effects on germination and growth of muskmelon (Cucumis melo L.) seedlings under greenhouse conditions. The treatments included pure peat moss (control), and composts prepared from guar (Cyammopsis tetragonoloba L.), jantar (Sesbania aculeate L.), and wheat (Triticum aestivum L) straw, at three air-filled porosity (AFP) levels (10, 15, and 20%) and rice (Oryza sativa) hulls at 10% AFP level. The second factor was the addition and devoid of nitrogen (N), phosphorus (P), and potassium (K) fertilizers. The addition of fertilizers in all potting media at 10% AFP level showed a small increase in total nitrogen, extractable phosphorus, and potassium. In the guar and jantar compost at 10% AFP, the values of total N (92.43 and 85.32%), extractable P (68.13 and 76.65%), and K (63.33 and 49%) were higher than in peat moss. It is concluded that composts of guar, jantar, wheat straw, and rice hulls at 10% AFP level can be used as a substitute of peat moss for growing muskmelon seedlings, but the addition of N, P, and K fertilizers is required in wheat straw and rice hulls compost for better nutrition of muskmelon seedlings.

Subcellular compartmentation of sugars in wheat leaves under the influence of salinity and boron toxicity

ABSTRACT In this study, long-term effects of salinity and high boron (B) on subcellular distribution of sugars in wheat leaves were investigated. Four treatments with three replications of each; control, high B, sodium chloride (NaCl) and NaCl + high B, respectively were established according to completely randomized design. Plants were grown hydroponically and harvested after 6 weeks onset of experiment. NaCl treatment markedly decreased the shoot fresh and dry weight compared to high B or NaCl + high Boron. It increased the sugar concentrations in subcellular compartments, whereas decreased in NaCl + high B. Contrary, NaCl either alone or in combination with high B decreased the sugar contents in whole leaf compared to control or high B. Overall, higher concentrations of sugars were observed in symplast compared to apoplast indicating the symplast as major compartment for sugar transport. Furthermore, wheat plants accumulate sugars in subcellular compartments to maintain their growth under stress conditions.