Sakshi Sharma

Phylogenetic diversity and metabolic potential of microbiome of natural healing clay from Chamliyal (J&K)

Clay therapy for skin disease treatment is an ancient practice popular worldwide as a cheap alternative to pharmaceutical products. Effectiveness of clay against skin problems has been linked to its mineral composition and to microbial activity. The clay–water paste of a holy shrine Chamliyal in the Jammu region of J&K, India is used as an ointment to treat different skin disorders particularly psoriasis. Using the 16 SrDNA amplicon pyrosequencing and whole-metagenome direct shotgun Illumina sequencing, microbial phylogeny and potential metabolic functions were catalogued for Chamliyal’s clay. Microbial diversity profile of the Chamliyal’s clay is similar to other medicinal clays, particularly Dead Sea; there is some uniqueness as well. Although Proteobacteria, Actinomycetes and Firmicutes are common inhabitants of all the clay types, sulphur- and iron-reducing bacteria like Deferribacterales are particular to clays used for skin healing. In the present study it is proposed that healing properties of clay may be due to the microbes and microbial genes associated with metabolism of minerals like iron and sulphur, that lead to mineral acquisition in the Chamliyal’s clay.

RETRACTED ARTICLE: A review of 45 candidate genes: association of single nucleotide polymorphism to schizophrenia risk

The following article has been retracted from publication in the Taylor & Francis journal New Genetics and Society. I. Priya, S. Sharma, I. Sharma, R. Mahajan and N. Kapoor, A review of 45 candidate genes: association of single nucleotide polymorphism to schizophrenia risk, New Genetics and Society https://doi.org/10.1080/14636778.2018.1481740. Version of Record published online 13 July 2018. The editorial office of the journal inadvertently processed the paper through the online submission system without proper peer review or requisite checks. This has now been remedied and the journal and publishers apologise to the authors that this occurred. Journal processes and checks have now been reviewed and updated so that all best efforts are made to ensure this does not occur again.

Molecular Modeling, Spectroscopic Investigations, and Computational Studies of DMSO solvated 7′-amino-1′,3′-dimethyl-2,2′,4′-trioxo-1′,2′,3′,4′,4a′,8a′-tetrahydrospiro[indoline-3,5′-pyrano[2,3-d]pyrimidine]-6′-carbonitrile

A combined experimental and theoretical study is performed on DMSO solvated 7′-amino-1′,3′-dimethyl- 2,2′,4′-trioxo-1′,2′,3′,4′,4a′,8a′-tetrahydrospiro[indoline-3,5′-pyrano[2,3-d]pyrimidine]-6′-carbonitrile. The compound is studied by NMR, IR spectroscopy, and single crystal X-ray analysis. The crystal structure of the molecule is stabilized by intermolecular N–H…N, N–H…O, and C–H…π interactions. In the crystal, the molecules form hydrogen-bonded chains running along the b axis of the unit cell. In the present work, we have applied density functional theory (DFT) to explore the nonlinear properties of the molecule. The harmonic vibrational frequencies are calculated and compared with experimental FT-IR frequencies. The observed and calculated frequencies are found to be in good agreement. The calculated values of the HOMO-LUMO energy gap shows that a charge transfer occurs within the molecule.

Synthesis, spectral characterization, and single crystal structure studies of biologically relevant bis-indoline heterocyclic scaffold

The new biologically relevant compound, 5,5″-methoxy-1′-methyl-1H,1″H-[3,3′:3′,3″-terindol]-2′(1′H)-one was synthesized, in 89% yield, by the one-pot reaction between 5-methoxyindole and 1-methylisatin in the presence of a low-cost and environmentally benign commercially available sulfamic acid as an organocatalyst and have been characterized by elemental analyses, IR spectra, and single crystal X-ray analysis. The crystals are orthorhombic, sp. gr. Pbca, Z = 8. The structure is stabilized by N–H···O and C–H···O interactions.

Synthesis, characterization, and crystal structure of 5,5″-Difluoro-1H,1″H-[3,3′:3′,3″-terindol]-2′(1′H)-one

The new indole derivative, 5,5′′-Difluoro-1H,1′′H-[3,3′:3′,3′′-terindol]-2′(1′H)-one C24H15F2N3O, is synthesized in 87% yield, and its crystal structure is determined by X-ray structure analysis. The crystals are monoclinic, sp. gr. P21/n, a = 15.4563(7), b = 10.8340(6), c = 16.4718(6) Å, β = 102.403(4)°, Z = 4. Bicyclic indole moieties form dihedral angle of 61.92(5)° with each other; the oxindole ring is twisted with respect to them at angles of 85.70(5)° and 75.62(5)°. The crystal structure is stabilized by N–H···O and C–H···O hydrogen bonds involving both the DMSO solvent molecules. In addition, one C–H···π interaction is observed.

Synthesis, spectroscopic characterization, and crystal structure of a novel indoline derivative

A new indoline derivative, tert-butyl 2′-amino-3′-cyano-6′-methyl-2-oxospiro[indoline-3,4′-pyran]-5′-carboxylate is eco-friendly synthesized, and its spectral properties and X-ray crystal structure are studied. In the molecule, the oxindole and pyran moieties are perpendicular to each other. The crystal structure is stabilized by intermolecular N−H···O and intramolecular C−H···O hydrogen bonds. Centrosymmetric dimer units are formed by intermolecular N−H···O hydrogen bonds. In addition, one C−H···π interaction is also observed.

X-ray studies of 2-amino-4-(3-nitrophenyl)-5-oxo-4,5-dihydropyrano[3,2-c] chromene-3-carbonitrile and 2-amino-7,7-dimethyl-4-(4-nitrophenyl)-5-oxo-5,6,7,8-tetrahydro-4H-chromene-3-carbonitrile

Two carbonitrile compounds, 2-amino-4-(3-nitrophenyl)-5-oxo-4,5-dihydropyrano[3,2-c] chromene-3-carbonitrile (I) and 2-amino-7,7-dimethyl-4-(4-nitrophenyl)-5-oxo-5,6,7,8-tetrahydro-4H-chromene-3-carbonitrile (II), were synthesized, and their crystal structures were determined by X-ray diffraction technique. The crystals are triclinic, a = 7.7970(12), 8.2171(7), b = 9.0390(10), 9.2238(7), c = 15.148(2), 14.5585(11) Å, α = 81.439(10)°, 74.895(6)°, β = 75.194(12)°, 87.392(6)°, γ = 76.151(11)°, 78.552(7)°, for I and II, respectively, Z = 2, sp. gr. P

X-ray studies of 2-amino-5-oxo-4-propyl-4,5-dihydropyrano[3,2-c]chromene-3-carbonitrile

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