Salvatore J. Arrigo
Medical University of South Carolina
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Featured researches published by Salvatore J. Arrigo.
Journal of Biomedical Science | 1998
Joel Gatlin; Salvatore J. Arrigo; Michael G. Schmidt
The maturation of human immunodeficiency type-1 virions is accomplished through the proteolytic processing of Gag and GagPol precursor proteins by the viral protease (PR). Since virions must be assembled at the cell surface from uncleaved precursor molecules, intracellular activation of PR must be inhibited. We have previously developed a system where the intracellular activity of PR, associated with GagPol, was inhibited by the expression of Gag in trans. The disproportionate synthesis of Gag inhibits the activation of PR in the cytoplasm. Sequences capable of mediating this inhibition were localized to capsid. In this communication, the region of HIV-1 capsid capable of mediating inhibition was further defined and shown to require the major homology region of capsid within Gag.
Journal of Biomedical Science | 1999
Kim M. Huffman; Salvatore J. Arrigo; Michael G. Schmidt
Rev has been shown to promote the export of HIV-1 RNAs from Xenopus oocyte nuclei, but a system to examine the direct effect of Rev on HIV-1 RNA export in mammalian somatic cells does not exist. In this report, the development of a cell-free RNA export system using COS cells is described. This system is capable of examining the movement of RNA from nuclei of COS cells transfected with an HIV-1 proviral construct into reconstituted cytosol from nontransfected cells. A reproducible preparation of nuclei free of residual cytoplasmic RNA is demonstrated. Export of RNA from these nuclei into reconstituted cell-free extracts was saturable and dependent on temperature and energy. Further validation of the system was obtained by confirming that the nuclear export of HIV-1-unspliced and partially spliced RNAs was dependent upon the expression of HIV-1 Rev and that the presence of Rev appeared to decrease the export of an HIV-1-spliced RNA. The system was also able to demonstrate that Rev did not appear to significantly enhance the export of an HIV-1 protease-containing RNA that has been shown to be dependent upon Rev for maximal expression. Consequently, the system appears useful for the examination of parameters of nuclear export of HIV-1 and cellular RNAs.
Journal of Virology | 1995
Salvatore J. Arrigo; Kim M. Huffman
Journal of Virology | 1994
Lia H. Campbell; Keith T. Borg; Julia K. Haines; Randall T. Moon; Daniel R. Schoenberg; Salvatore J. Arrigo
Virology | 1997
Justin P. Favaro; Salvatore J. Arrigo
Virology | 1997
Keith T. Borg; Justin P. Favaro; Salvatore J. Arrigo
Virology | 1998
Justin P. Favaro; Keith T. Borg; Salvatore J. Arrigo; Michael G. Schmidt
Virology | 1996
Lia H. Campbell; Keith T. Borg; Salvatore J. Arrigo
Virology | 1999
Justin P. Favaro; Frank Maldarelli; Salvatore J. Arrigo; Michael G. Schmidt
Virology | 1998
Joel Gatlin; Salvatore J. Arrigo; Michael G. Schmidt