Sameer S. Rahatekar

Optical microstructure and viscosity enhancement for an epoxy resin matrix containing multiwall carbon nanotubes

This paper describes rheological measurements and associated optical microstructural observations of multiwall carbon nanotubes (MWCNTs) suspended in an epoxy resin matrix. The base epoxy resin was found to be essentially Newtonian, and the progressive incorporation of nanotubes enhanced the low shear rate viscosity of the suspension by nearly two decades. At higher shear rates, the suspension viscosity asymptotically thinned to the viscosity of the matrix alone. The low shear rate viscosity enhancement was correlated with the optical observations of interconnected aggregates of carbon nanotubes, which themselves were induced by the low shear conditions. Intermediate shear rates resulted in a reduction in the size of the aggregates. High shear rates appeared to cause near-complete dispersal of the aggregates. From these results it is conjectured that for this suspension, shear thinning is connected with the breaking of the interconnected networks between nanotubes and or aggregates of nanotubes, and not b...

Shear and extensional rheology of cellulose/ionic liquid solutions

In this study, we characterize the shear and extensional rheology of dilute to semidilute solutions of cellulose in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIAc). In steady shear flow, the semidilute solutions exhibit shear thinning, and the high-frequency complex modulus measured in small amplitude oscillatory shear flow exhibits the characteristic scaling expected for solutions of semiflexible chains. Flow curves of the steady shear viscosity plotted against shear rate closely follow the frequency dependence of the complex viscosity acquired using oscillatory shear, thus satisfying the empirical Cox-Merz rule. We use capillary thinning rheometry (CaBER) to characterize the relaxation times and apparent extensional viscosities of the semidilute cellulose solutions in a uniaxial extensional flow that mimics the dynamics encountered in the spin-line during fiber spinning processes. The apparent extensional viscosity and characteristic relaxation times of the semidilute cellulose/EMIAc solutions increase dramatically as the solutions enter the entangled concentration regime at which fiber spinning becomes viable.

Mesoscale modeling of electrical percolation in fiber-filled systems

The research described in this paper primarily involves mesoscale simulations: dissipative particle dynamics (DPD) of packed assemblies of oriented fibers suspended in a viscous medium. Computer simulations have been performed in order to explore how the aspect ratio and degree of fiber alignment affect the critical volume fraction (percolation threshold) required to achieve electrical conductivity. The fiber network impedance was assessed using Monte Carlo simulations after establishing the structural arrangement with DPD. The predictions are compared with the predictions of classical percolation theory and found to be in close agreement. The approach is thus validated and can be extended to systems that cannot be tackled analytically; in particular, the work is motivated by long-standing interest in materials which display a complex percolation behavior.

Directing chondrogenesis of stem cells with specific blends of cellulose and silk

Biomaterials that can stimulate stem cell differentiation without growth factor supplementation provide potent and cost-effective scaffolds for regenerative medicine. We hypothesize that a scaffold prepared from cellulose and silk blends can direct stem cell chondrogenic fate. We systematically prepared cellulose blends with silk at different compositions using an environmentally benign processing method based on ionic liquids as a common solvent. We tested the effect of blend compositions on the physical properties of the materials as well as on their ability to support mesenchymal stem cell (MSC) growth and chondrogenic differentiation. The stiffness and tensile strength of cellulose was significantly reduced by blending with silk. The characterized materials were tested using MSCs derived from four different patients. Growing MSCs on a specific blend combination of cellulose and silk in a 75:25 ratio significantly upregulated the chondrogenic marker genes SOX9, aggrecan, and type II collagen in the absence of specific growth factors. This chondrogenic effect was neither found with neat cellulose nor the cellulose/silk 50:50 blend composition. No adipogenic or osteogenic differentiation was detected on the blends, suggesting that the cellulose/silk 75:25 blend induced specific stem cell differentiation into the chondrogenic lineage without addition of the soluble growth factor TGF-β. The cellulose/silk blend we identified can be used both for in vitro tissue engineering and as an implantable device for stimulating endogenous stem cells to initiate cartilage repair.

Numerical simulation of pressure variation and resin flow in injection pultrusion

Injection pultrusion is a continuous process for manufacturing composite materials. To produce good quality parts it is essential that complete wet out of the reinforcement fibers is achieved in the injection chamber. To achieve good wet out of the fibers, the magnitude of the resin injection pressure is extremely important. The present study is focused on the effects of pull speed, fiber volume fraction, resin viscosity and compression ratio (taper) of the injection chamber on resin fiber wet out within the injection chamber for polyester-glass roving composites. The recommended injection pressures for complete wet out are predicted for a wide and comprehensive variety of processing variables; this work is novel in that it has new and comprehensive results not available in the existing literature. Darcys law is used to model the fiber/resin system of injection pultrusion. The Gutowski [Gutowski, T.G., Morigaki, T. and Cai, Z. (1987). The Consolidation of Laminate Composites, Journal of Composite Materials, 21(7): 172-187.] permeability model is used to determine the transverse permeability and the Kozeny-Carman [Carman, P.C. (1939). Flow Through Granular Beds, Trans. Int. Chem. Eng., 15: 150-166.] model is used to predict the longitudinal permeability. The finite volume method is used to predict the resin pressure field, resin velocity field, and resin moving flow front location.

Chitosan silk-based three-dimensional scaffolds containing gentamicin-encapsulated calcium alginate beads for drug administration and blood compatibility

In the present study gentamicin was encapsulated within calcium alginate beads and incorporated into porous chitosan, gelatin, double-hybrid silk fibroin, chitosan/gelatin and double-hybrid silk fibroin/chitosan scaffolds. Physiochemical, morphological and biological properties of fabricated amenable model systems were evaluated, revealing hemocompatible nature of double-hybrid silk fibroin/chitosan and double-hybrid silk fibroin scaffolds of hemolysis %<5 and porosity >85%. Fourier transform infrared results confirmed the blend formation and scanning electron microscope images showed good interconnectivity. Double-hybrid silk fibroin/chitosan-blended scaffold shows higher compressive strength and compressive modulus than other fabricated scaffolds. A comparative drug release profile of fabricated scaffolds revealed that double-hybrid silk fibroin/chitosan scaffold is a pertinent model system because of its prolonged drug release, optimal hemocompatability and high compressive modulus.

Ionic liquids-based processing of electrically conducting chitin nanocomposite scaffolds for stem cell growth

In the present study, we have successfully combined the biocompatible properties of chitin with the high electrical conductivity of carbon nanotubes (CNTs) by mixing them using an imidazolium-based ionic liquid as a common solvent/dispersion medium. The resulting nanocomposites demonstrated uniform distribution of CNTs, as shown by scanning electron microscopy (SEM) and optical microscopy. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction confirmed the α-crystal structure of chitin in the regenerated chitin nanocomposite scaffolds. Increased CNT concentration in the chitin matrix resulted in higher conductivity of the scaffolds. Human mesenchymal stem cells adhered to, and proliferated on, chitin–CNT nanocomposites with different ratios. Cell growth in the first 3 days was similar on all composites at a range of (0.01 to 0.07) weight fraction of CNT. However, composites at a 0.1 weight fraction of CNTs showed reduced cell attachment. There was a significant increase in cell proliferation using 0.07 weight fraction CNT composites, suggesting a stem cell enhancing function for CNTs at this concentration. In conclusion, the ionic liquid allowed the uniform dispersion of CNTs and dissolution of chitin to create a biocompatible, electrically conducting scaffold permissive for mesenchymal stem cell function. This method will enable the fabrication of chitin-based advanced multifunctional biocompatible scaffolds where electrical conduction is critical for tissue function.

Shear-induced anisotropy of concentrated multiwalled carbon nanotube suspensions using x-ray scattering

X-ray scattering is used to measure particle orientation in concentrated multiwalled carbon nanotube (MWNT) suspensions under shear flow. MWNTs were dispersed in a Newtonian suspending fluid (uncured epoxy). The dispersions exhibit shear thinning, approaching the matrix viscosity at high shear rates. This is accompanied by progressive development of MWNT orientation along the flow direction with increasing shear rate. The impact of MWNT aspect ratio and concentration on steady-state orientation is explored. In one sample (2 wt. % dispersion of short MWNTs), orientation was measured in both the flow-gradient (1-2) and flow-vorticity (1-3) planes of shear flow to provide a more complete picture of the three-dimensional orientation state. Also in this sample, 1-3 plane measurements were conducted using both small- and wide-angle x-ray scattering (SAXS and WAXS). While the two methods produce qualitatively similar results, WAXS-derived measures of flow-induced anisotropy are consistently larger than SAXS data...

Drawdown prepreg coating method using epoxy terminated butadiene nitrile rubber to improve fracture toughness of glass epoxy composites

Laminates of fibre-reinforced prepreg have excellent in-plane mechanical properties, but have inadequate performance in the through thickness direction. Here, we address this issue by application of epoxy-terminated butadiene nitrile (ETBN) liquid rubber between the prepreg laminae using an automatic draw bar coating technique. Test results reveal that by adding ETBN in small quantities in the range of 9.33–61.33 g/m2, the interlaminar critical energy release rates (GIc and GIIc) are improved by up to 122% in mode-I and 49% in mode-II. Moreover, this finding is further supported by the dynamic mechanical analysis thermograms that clearly indicate that coating has not altered the Tg of ETBN-coated samples. Scanning electron microscopic analysis of fracture surfaces showed that rubber particles formed micro cavitations in the epoxy, causing localised rubber rich regions. These resin-rich regions require more energy to fracture, resulting in increased toughness of the glass epoxy prepreg systems.

Injection Pultrusion Simulation for Polyester/Glass Mat/Rovings/Mat Composites

Producing quality pultruded parts requires that complete wet out of the reinforcement fibers is achieved in the injection chamber. The magnitude of the injection pressure is extremely important for achieving good wet out of the fibers. This work focused on the impact of pull speed, resin viscosity, and product thickness on resin fiber wet out in the injection chamber for a polyester resin (mat/roving/mat) composite. Recommended injection pressures for complete wet out are predicted for given sets of processing parameters for polyester/glass fiber (mat/rovings/mat) composites. Flow through porous media (Darcy’s law) is employed to model the fiber/resin system of injection pultrusion. The governing equations are solved, by using the finite volume method, to predict the resin pressure, resin velocity field, and resin moving flow front location. The Gutowski (Gutowski, T.G., Morigaki, T. and Cai, Z. (1987). The Consolidation of Laminate Composites, Journal of Composite Materials. 21(7): 172-187) permeability model is used to determine the permeability in the roving layer and the Kozeny-Carman (Carman, P.C. (1939). Flow through Granular Beds, Trans. Int. Chem. Eng., 15: 150-166) model is used to predict the permeability in the mat layers.