Sameh S. Ali

Effective bio-pretreatment of sawdust waste with a novel microbial consortium for enhanced biomethanation

Anaerobic digestion (AD) is considered an efficient cost-effective technology for sustainable biogas production from lignocellulosic wastes. A novel lignocellulosic degradation microbial consortium (LCDC) was isolated from rotten sawdust, and further used for sawdust pretreatment prior to AD. Results showed that pretreatment of sawdust for 10days led to significant reduction in cellulose, hemicelluloses, and lignin contents by 37.5%, 39.6%, and 56.7%, respectively, with respect to the control. In addition, the pretreatment enhanced cumulative biogas yield, which reached its maximum value of 312.0Lkg-1VS after 28days of AD (25.6% higher than the corresponding control). Moreover, the maximum significant cumulative methane yield was recorded after 28days of AD of the pretreated sawdust (155.2Lkg-1VS), which represented 72.6% higher than the corresponding control. Significantly higher biomethane yield from sawdust pretreated with LCDC confirms that this process is more economical than the previous reports.

Synthesized zinc peroxide nanoparticles (ZnO 2 -NPs): a novel antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory approach toward polymicrobial burn wounds

Increasing of multidrug resistance (MDR) remains an intractable challenge for burn patients. Innovative nanomaterials are also in high demand for the development of new antimicrobial biomaterials that inevitably have opened new therapeutic horizons in medical approaches and lead to many efforts for synthesizing new metal oxide nanoparticles (NPs) for better control of the MDR associated with the polymicrobial burn wounds. Recently, it seems that metal oxides can truly be considered as highly efficient inorganic agents with antimicrobial properties. In this study, zinc peroxide NPs (ZnO2-NPs) were synthesized using the co-precipitation method. Synthesized ZnO2-NPs were characterized by X-ray diffraction, Fourier transformed infrared, transmission electron microscopy, thermogravimetric analysis, differential scanning calorimetry, and ultraviolet-visible spectroscopy. The characterization techniques revealed synthesis of the pure phase of non-agglomerated ZnO2-NPs having sizes in the range of 15–25 nm with a transition temperature of 211°C. Antimicrobial activity of ZnO2-NPs was determined against MDR Pseudomonas aeruginosa (PA) and Aspergillus niger (AN) strains isolated from burn wound infections. Both strains, PA6 and AN4, were found to be more susceptible strains to ZnO2-NPs. In addition, a significant decrease in elastase and keratinase activities was recorded with increased concentrations of ZnO2-NPs until 200 µg/mL. ZnO2-NPs revealed a significant anti-inflammatory activity against PA6 and AN4 strains as demonstrated by membrane stabilization, albumin denaturation, and proteinase inhibition. Moreover, the results of in vivo histopathology assessment confirmed the potential role of ZnO2-NPs in the improvement of skin wound healing in the experimental animal models. Clearly, the synthesized ZnO2-NPs have demonstrated a competitive capability as antimicrobial, anti-elastase, anti-keratinase, and anti-inflammatory candidates, suggesting that the ZnO2-NPs are promising metal oxides that are potentially valued for biomedical applications.

Drug resistance profile and molecular characterization of extended spectrum beta-lactamase (ESβL)-producing Pseudomonas aeruginosa isolated from burn wound infections. Essential oils and their potential for utilization

OBJECTIVES Pseudomonas aeruginosa producing extended spectrum β-lactamase (ESβL) enzyme had the ability for antimicrobial resistance mechanisms and its multidrug-resistant (MDR) phenotype, has been increasingly reported as a major clinical concern worldwide. The aim of this study was to (i) characterize ESβL-producing MDR P. aeruginosa isolated from burn wound infections phenotypically and molecularly, (ii) evaluate the antibacterial activity of some essential oils (EOs) against selected ESβL-producing drug resistant P. aeruginosa and (iii) characterize a promising EO. METHODS Identification and antibiotic susceptibility tests were performed for all isolates. ESβL production was detected phenotypically by an initial screening test (IST) and a phenotypic confirmatory test (PCT). Additionally, ESβL-producing isolates were also characterized molecularly. The antibacterial activity was detected using a disc diffusion method. Mechanisms of antibacterial action, the fatty acid profile, and functional groups characterization of the promising EO were analyzed using scanning and transmission electron microscopy (SEM & TEM), gas chromatography-mass spectrometry (GC-MS), and Fourier transform infrared (FTIR) spectroscopy, respectively. RESULTS A total of 50 non duplicated P. aeruginosa isolates from the wound samples of burn patients were identified. Of these, MDR and pan-drug resistance (PDR) showed a high prevalence in 38 (76%) isolates obtained from 10 clusters, while 21 (42%) were identified as ESβL-producing MDR or PDR P. aeruginosa isolates. Phenotypic detection of ESβL production showed that 20% were considered positive ESβL-producing P. aeruginosa using the IST, and were increased to 56% by the PCT. The most prevalent ESβL-encoding gene was blaOXA-2 (60.7%), followed by blaIMP-7 (53.6%) and blaOXA-50 (42.8%). Ginger oil is the most efficient antibacterial agent and its antibacterial action mechanism is attributed to the morphological changes in bacterial cells. The oil characterization revealed that 9,12-Octadecadienoic acid methyl ester is the major fatty acid (50.49%) identified. CONCLUSION The high incidence of drug-resistance in ESβL-producing P. aeruginosa isolated from burn wounds is alarming. As proven in vitro, EOs may represent promising natural alternatives against ESβL-producing PDR or MDR P. aeruginosa isolates.