Sanae Masuko

Preparation of Microspheres of Poly(Glycidyl Methacrylate) and its Derivatives as Carriers for Immobilized Proteins

Microspheres of poly(glycidyl methacrylate) and its derivatives have been prepared. The diameter can be varied in the range from 0.5 to 5 micron. Proteins were immobilized on the microspheres by various processes. The maximum amount of covalently immobilized gamma-globulin on the microspheres was found to be about 6 mg/m2. It was shown that dyed microspheres with a diameter of 2-4 micron are applicable to immunological agglutination tests on a microplate as carrier particles substituting for red blood cells.

The Application of Luminol-Bound Microspheres for the Quantitative Analysis of Toxic Oxygen within Phagosomes

Hydrophilic microspheres, with a diameter of about 2μm, composed of glyceryl methacrylate and methacrylic acid were prepared. The microspheres could be stably stored in dispersed state and demonstrated little non-specific adherance to cell membranes. Luminol-bound microspheres, prepared by covalently binding luminol to microspheres, were phagocytosed by phagocytic cells without opsonization. Luminol bound to the microspheres was oxidized by toxic oxygen within phagosomes resulting in the generation of chem iluminescence. The chemiluminescence elicited by luminol-bound micro spheres was an effective method of measuring the candidacidal activity of the cells. The difference in phagosomal toxic oxygen versus extracellular toxic oxy gen was examined.