Sandy Wyllie-Echeverria

Estimating basal area coverage of subtidal seagrass beds using underwater videography

Abstract Although seagrasses have been identified as vital living marine resources, their distribution has not been rigorously quantified at many locations. This fact is often due to the high cost of sampling seagrass habitats, especially those with deep-water plants that cannot be sensed from aerial platforms. We present a cost-effective method of estimating basal area coverage of submersed vegetation that uses differential global positioning system data linked to underwater video images of the bottom. Our sampling design and statistical procedures are identical to estimating proportions using cluster sampling with unequal cluster sizes. This method has several advantages over other techniques: (1) confidence intervals around basal area coverage estimates permit hypothesis testing of changes over time; (2) sampling efficiency is better than simple random sampling with quadrats; (3) deep-water zones out of the range of aerial platforms can be sampled; (4) video images provide positive identification of plants which is not possible with acoustic techniques; and (5) the techniques provide a permanent archive of visual images that can be analyzed for other bottom attributes, such as other vegetation, macro-invertebrates, and gross sediment types. This method has some limitations: (1) it is not possible to sample extremely shallow or turbid waters and under some physical structures; (2) it is impractical to sample very large regions; (3) errors in differential global positioning system data must be accounted for; and (4) seagrass density must be measured subjectively. We illustrate our sampling methods and data analysis with an example from Puget Sound, Washington, USA.

Seagrass Conservation Biology: An Interdisciplinary Science for Protection of the Seagrass Biome

In the past three decades seagrass research has adopted several disciplines and matured into a global science. One of the approaches we can use to focus our science to benefit the management and protection of seagrass is that of Conservation Biology; a proactive field of science bringing together academic, government, and nongovernmental organizations from a wide range of disciplines to understand and conserve biodiversity. This relatively recent field synthesises and directs insights from many disciplines for direct application to the protection and conservation of species, communities, and biomes (Fig. 1). While the primary focus for conservation biology comes from ecology, genetics, landscape ecology, population biology and taxonomy, the discipline also incorporates analytical procedures associated with the social sciences, biogeography, and evolutionary biology (Soule and Wilcox, 1980; Soule, 1985; Meffe and Carroll, 1994; Primack, 2000). Conservation biology recognizes that humans derive both extractive and intrinsic benefit from the natural world and embraces methods and analyses utilized in fisheries science, agriculture, anthropology, economics, law, philosophy, and sociology. Today, unlike traditional approaches that were rooted in the preservation and management of selected species, conservation biologists are advising natural resource managers to focus more on an ecosystem approach that includes entire biomes, and to recognize that public trust demands comprehensive protection of biodiversity as much as sustaining the yield of harvestable organisms. Conservation biology endeavors to maintain and protect biodiversity at all spatial scales, including a variety of little understood and often overlooked life forms. In the broader meaning of biodiversity we are interested in conserving ecological services as much as life forms (sensu Randall, 1986).

Managing marine disease emergencies in an era of rapid change

Infectious marine diseases can decimate populations and are increasing among some taxa due to global change and our increasing reliance on marine environments. Marine diseases become emergencies when significant ecological, economic or social impacts occur. We can prepare for and manage these emergencies through improved surveillance, and the development and iterative refinement of approaches to mitigate disease and its impacts. Improving surveillance requires fast, accurate diagnoses, forecasting disease risk and real-time monitoring of disease-promoting environmental conditions. Diversifying impact mitigation involves increasing host resilience to disease, reducing pathogen abundance and managing environmental factors that facilitate disease. Disease surveillance and mitigation can be adaptive if informed by research advances and catalysed by communication among observers, researchers and decision-makers using information-sharing platforms. Recent increases in the awareness of the threats posed by marine diseases may lead to policy frameworks that facilitate the responses and management that marine disease emergencies require.

Competition between the invasive macrophyte Caulerpa taxifolia and the seagrass Posidonia oceanica : contrasting strategies

BackgroundPlant defense strategy is usually a result of trade-offs between growth and differentiation (i.e. Optimal Defense Theory – ODT, Growth Differentiation Balance hypothesis – GDB, Plant Apparency Theory – PAT). Interaction between the introduced green alga Caulerpa taxifolia and the endemic seagrass Posidonia oceanica in the Mediterranean Sea offers the opportunity to investigate the plausibility of these theories. We have accordingly investigated defense metabolite content and growth year-round, on the basis of an interaction gradient.ResultsWhen in competition with P. oceanica, C. taxifolia exhibits increased frond length and decreased Caulerpenyne – CYN content (major terpene compound). In contrast, the length of P. oceanica leaves decreases when in competition with C. taxifolia. However, the turnover is faster, resulting in a reduction of leaf longevity and an increase on the number of leaves produced per year. The primary production is therefore enhanced by the presence of C. taxifolia. While the overall concentration of phenolic compounds does not decline, there is an increase in some phenolic compounds (including ferulic acid and a methyl 12-acetoxyricinoleate) and the density of tannin cells.ConclusionInterference between these two species determines the reaction of both, confirming that they compete for space and/or resources. C. taxifolia invests in growth rather than in chemical defense, more or less matching the assumptions of the ODT and/or PAT theories. In contrast, P. oceanica apparently invests in defense rather than growth, as predicted by the GDB hypothesis. However, on the basis of closer scrutiny of our results, the possibility that P. oceanica is successful in finding a compromise between more growth and more defense cannot be ruled out.

Population Structure and Genetic Diversity among Eelgrass (Zostera marina) Beds and Depths in San Francisco Bay

The seagrass Zostera marina is widely distributed in coastal regions throughout much of the northern hemisphere, forms the foundation of an important ecological habitat, and is suffering population declines. Studies in the Atlantic and Pacific oceans indicate that the degree of population genetic differentiation is location dependent. San Francisco Bay, California, USA, is a high-current, high-wind environment where rafting of seed-bearing shoots has the potential to enhance genetic connectivity among Z. marina populations. We tested Z. marina from six locations, including one annual population, within the bay to assess population differentiation and to compare levels of within-population genetic diversity. Using 7 microsatellite loci, we found significant differentiation among all populations. The annual population had significantly higher clonal diversity than the others but showed no detectible differences in heterozygosity or allelic richness. There appears to be sufficient input of genetic variation through sexual reproduction or immigration into the perennial populations to prevent significant declines in the number and frequency of alleles. In additional depth comparisons, we found differentiation among deep and shallow portions in 1 of 3 beds evaluated. Genetic drift, sweepstakes recruitment, dispersal limitation, and possibly natural selection may have combined to produce genetic differentiation over a spatial scale of 3-30 km in Z. marina. This implies that the scale of genetic differentiation may be smaller than expected for seagrasses in other locations too. We suggest that populations in close proximity may not be interchangeable for use as restoration material.

Field and Remote-Sensing Assessment of Mangrove Forests and Seagrass Beds in the Northwestern Part of the United Arab Emirates

Abstract Mangrove stands and seagrass beds grow along the coasts of the United Arab Emirates (UAE). These marine plant species are concentrated in specific tidal zones along sheltered intertidal coastlines in association with estuaries and lagoons; mangroves fringe the coastline encroaching into the lower intertidal region, whereas seagrasses populate the adjacent deeper water. In most cases, the distribution of mangroves and seagrasses does not overlap. It is important to monitor the geographic extent and health of mangrove forests and seagrass beds, which serve as an important habitat for juvenile marine species. Remote-sensing data for the Khor Al Bazam area in the vicinity of Abu Dhabi, UAE, covering the years of 1994, 2000, and 2003, were used to determine the change in mangrove and seagrass cover. Since 1994, there has been an increase in mangrove cover, likely because of plantation activity, the closure of nearby shipyards, and an increase in public awareness regarding mangrove preservation. Although more difficult to determine, a combination of remote sensing and ground-truthing indicates that the seagrass beds within the study area have likely increased in real extent over the same time period.

Distribution and Performance of the Nonnative Seagrass Zostera japonica across a Tidal Height Gradient on Shaw Island, Washington

Abstract: In the Northeast Pacific the nonnative seagrass Zostera japonica frequently exists at the same sites as the native seagrass Zostera marina. Although at some sites their vertical distributions overlap, at most sites in the Pacific Northwest there is a distinctive unvegetated zone between them. The objective of this study was to better understand why a gap between the lower limit of Z. japonica and the upper limit of Z. marina exists. To address this issue we carried out transplant experiments, conducted in situ monitoring of existing Z. japonica patches, and collected sediment samples at South Beach on Shaw Island, Washington, during the spring and summer of 2006. Transplant and in situ monitoring data indicate that survival and performance of Z. japonica are reduced lower in the intertidal zone. In addition, Z. japonica patches tended to be smaller and more spaced out at lower tidal heights. Although we found no Z. japonica seeds within or outside extant Z. japonica patches, high transplant mortality indicates that Z. japonica dispersal limitation is an unlikely cause of the unvegetated gap zone. Our field observations further suggest that herbivory, biotarbation, and epiphytes are unlikely causes of the gap pattern at our study site. Instead, we hypothesize that light limitation prevents Z. japonica from occurring lower in the intertidal. A review of published vertical distribution data for both Zostera species indicates that the lower limit of Z. japonica is relatively invariant among sites. In contrast, the upper limit of Z. marina is highly variable, ranging by more man 4 m within some subregions in Washington State. Consequently we hypodiesize that intersite variability in the vertical distribution of Z. marina is the primary driver of spatial variability in the presence of the unvegetated gap.

The seagrass (Zostera marina [zosteraceae]) industry of Nova Scotia (1907–1960)

Wild gathering of the leaves of the submerged marine monocotyledon Zostera marina L. once formed the basis of a vigorous insulation industry in North America. Since European colonization, fishing communities used detached leaves, deposited on the beach by tide and wind, as green manure and domestic insulation, but beginning in the late 1800s, these leaves were utilized in a commercial insulating product. Two companies manufactured seagrass “quilts” that were installed in many buildings of the period including some of the first skyscrapers. We here describe the importance of seagrass gathering for the coastal community of Yarmouth County, Nova Scotia, Canada. Interviews with older residents and analysis of county and company archives facilitate the reconstruction of what was once a seasonally important activity. Our findings have direct application to global seagrass protection initiatives.RésuméL’assemblage sauvage des feuilles submergés du monocotyledon marine, Zostera marina formait a un moment les bases d’une industrie d’isolation en Amérique du Nord. Depuis la colonisation Européenne, des communautés de pêcheurs utilisaient des feuilles détachées, déposées sur la plage par la marée et le vent comme engrais vert et isolation domestique; mais commençant à la fin des années 1800, ces feuilles séparées furent utilisées dans un produit commercial insultant. Deux compagnies manufacturaient des “édredons” de pailleule qui furent installées dans plusieurs bâtiments de l’époque y compris dans certains des grattes-ciel. Nous décrirons ici l’importance de l’assemblage de la pailleule pour la communauté côtière de Yarmouth County, Nova Scotia, Canada. Des entretiens avec des résidents d’un certain âge ainsi qu’une analyse du département et des archives de compagnies, facilitent la reconstruction de ce qui était à une époque, une activité saisonnière importante. Nos découvertes ont une application directes sur les initiatives de la protection globale de la pailleule.

Host demography influences the prevalence and severity of eelgrass wasting disease

Many marine pathogens are opportunists, present in the environment, but causing disease only under certain conditions such as immunosuppression due to environmental stress or host factors such as age. In the temperate eelgrass Zostera marina, the opportunistic labyrinthulomycete pathogen Labyrinthula zosterae is present in many populations and occasionally causes severe epidemics of wasting disease; however, risk factors associated with these epidemics are unknown. We conducted both field surveys and experimental manipulations to examine the effect of leaf age (inferred from leaf size) on wasting disease prevalence and severity in Z. marina across sites in the San Juan Archipelago, Washington, USA. We confirmed that lesions observed in the field were caused by active Labyrinthula infections both by identifying the etiologic agent through histology and by performing inoculations with cultures of Labyrinthula spp. isolated from observed lesions. We found that disease prevalence increased at shallower depths and with greater leaf size at all sites, and this effect was more pronounced at declining sites. Experimental inoculations with 2 strains of L. zosterae confirmed an increased susceptibility of older leaves to infection. Overall, this pattern suggests that mature beds and shallow beds of eelgrass may be especially susceptible to outbreaks of wasting disease. The study highlights the importance of considering host and environmental factors when evaluating risk of disease from opportunistic pathogens.

Emergency response for marine diseases

Marine diseases can decimate populations and can have substantial ecological, economic, and social impacts. Recent disease outbreaks in marine mammals, shellfish, sponges, seagrasses, crustaceans, corals, and fishes demonstrate the potential for catastrophic effects, including reduced biodiversity,