Sangram Keshari Samal

Cationic polymers and their therapeutic potential

The last decade has witnessed enormous research focused on cationic polymers. Cationic polymers are the subject of intense research as non-viral gene delivery systems, due to their flexible properties, facile synthesis, robustness and proven gene delivery efficiency. Here, we review the most recent scientific advances in cationic polymers and their derivatives not only for gene delivery purposes but also for various alternative therapeutic applications. An overview of the synthesis and preparation of cationic polymers is provided along with their inherent bioactive and intrinsic therapeutic potential. In addition, cationic polymer based biomedical materials are covered. Major progress in the fields of drug and gene delivery as well as tissue engineering applications is summarized in the present review.

New antimicrobial chitosan derivatives for wound dressing applications.

Chitosan is a non-toxic, biocompatible, biodegradable natural cationic polymer known for its low imunogenicity, antimicrobial, antioxidant effects and wound-healing activity. To improve its therapeutic potential, new chitosan-sulfonamide derivatives have been designed to develop new wound dressing biomaterials. The structural, morphological and physico-chemical properties of synthesized chitosan derivatives were analyzed by FT-IR, (1)H NMR spectroscopy, scanning electron microscopy, swelling ability and porosity. Antimicrobial, in vivo testing and biodegradation behavior have been also performed. The chitosan derivative membranes showed improved swelling and biodegradation rate, which are important characteristics required for the wound healing process. The antimicrobial assay evidenced that chitosan-based sulfadiazine, sulfadimethoxine and sulfamethoxazole derivatives were the most active. The MTT assay showed that some of chitosan derivatives are nontoxic. Furthermore, the in vivo study on burn wound model induced in Wistar rats demonstrated an improved healing effect and enhanced epithelialization of chitosan-sulfonamide derivatives compared to neat chitosan. The obtained results strongly recommend the use of some of the newly developed chitosan derivatives as antimicrobial wound dressing biomaterials.

Biomimetic Magnetic Silk Scaffolds

Magnetic silk fibroin protein (SFP) scaffolds integrating magnetic materials and featuring magnetic gradients were prepared for potential utility in magnetic-field assisted tissue engineering. Magnetic nanoparticles (MNPs) were introduced into SFP scaffolds via dip-coating methods, resulting in magnetic SFP scaffolds with different strengths of magnetization. Magnetic SFP scaffolds showed excellent hyperthermia properties achieving temperature increases up to 8 °C in about 100 s. The scaffolds were not toxic to osteogenic cells and improved cell adhesion and proliferation. These findings suggest that tailored magnetized silk-based biomaterials can be engineered with interesting features for biomaterials and tissue-engineering applications.

Chitosan hydrogels enriched with polyphenols: Antibacterial activity, cell adhesion and growth and mineralization.

Injectable hydrogels for bone regeneration consisting of chitosan, sodium beta-glycerophosphate (Na-β-GP) and alkaline phosphatase (ALP) were enriched with the polyphenols phloroglucinol (PG) and gallic acid (GA) and characterized physicochemically and biologically with respect to properties relevant for applications in bone regeneration, namely gelation kinetics, mineralizability, antioxidant properties, antibacterial activity, cytocompatibility and ability to support adhesion and growth of human osteoblast-like MG63 cells. Enrichment with PG and GA had no negative effect on gelation kinetics and mineralizability. PG and GA both enhanced antioxidant activity of unmineralized hydrogels. Mineralization reduced antioxidant activity of hydrogels containing GA. Hydrogels containing GA, PG and without polyphenols reduced colony forming ability of Escherichia coli after 1h, 3h and 6h incubation and slowed E. coli growth in liquid culture for 150min. Hydrogels containing GA were cytotoxic and supported cell growth more poorly than polyphenol-free hydrogels. PG had no negative effect on cell adhesion and growth.

Core–sheath structured electrospun nanofibrous membranes for oil–water separation

In recent years, both the increasing frequency of oil spill accidents and the urgency to deal seriously with industrial oil-polluted water, encouraged material scientists to design highly efficient, cost effective oil–water separation technologies. We report on electrospun nanofibrous membranes which are composed of core–sheath structured cellulose-acetate (CA)–polyimide (PI) nanofibers. On the surface of the CA–PI fibers a fluorinated polybenzoxazine (F-PBZ) functional layer, in which silica nanoparticles (SNPs) were incorporated, has been applied. Compared with F-PBZ/SNP modified CA fibers reported before for the separation of oil from water, the PI-core of the core–shell F-PBZ/SNP/CA–PI fibers makes the membranes much stronger, being a significant asset in their use. Nanofibrous membranes with a tensile strength higher than 200 MPa, a high water contact angle of 160° and an extremely low oil contact angle of 0° were obtained. F-PBZ/SNP/CA–PI membranes seemed very suitable for gravity-driven oil–water separation as fast and efficient separation (>99%) of oil from water was achieved for various oil–water mixtures. The designed core–sheath structured electrospun nanofibrous membranes may become interesting materials for the treatment of industrial oil-polluted water.

Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol® to endow antibacterial properties and promote mineralization

Hydrogels offer several advantages as biomaterials for bone regeneration, including ease of incorporation of soluble substances such as mineralization-promoting enzymes and antibacterial agents. Mineralization with calcium phosphate (CaP) increases bioactivity, while antibacterial activity reduces the risk of infection. Here, gellan gum (GG) hydrogels were enriched with alkaline phosphatase (ALP) and/or Seanol(®), a seaweed extract rich in phlorotannins (brown algae-derived polyphenols), to induce mineralization with CaP and increase antibacterial activity, respectively. The sample groups were unmineralized hydrogels, denoted as GG, GG/ALP, GG/Seanol and GG/Seanol/ALP, and hydrogels incubated in mineralization medium (0.1 M calcium glycerophosphate), denoted as GG/ALP_min, GG/Seanol_min and GG/Seanol/ALP_min. Seanol(®) enhanced mineralization with CaP and also increased compressive modulus. Seanol(®) and ALP interacted in a non-covalent manner. Release of Seanol(®) occurred in a burst phase and was impeded by ALP-mediated mineralization. Groups GG/Seanol and GG/ALP/Seanol exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus. GG/Seanol/ALP_min, but not GG/Seanol_min, retained some antibacterial activity. Eluates taken from groups GG/ALP_min, GG/Seanol_min and GG/ALP/Seanol_min displayed comparable cytotoxicity towards MG-63 osteoblast-like cells. These results suggest that enrichment of hydrogel biomaterials with phlorotannin-rich extracts is a promising strategy to increase mineralizability and antibacterial activity.

Laser-assisted photoporation: fundamentals, technological advances and applications

Abstract Laser-assisted photoporation is a promising technique that is receiving increasing attention for the delivery of membrane impermeable nanoscopic substances into living cells. Photoporation is based on the generation of localized transient pores in the cell membrane using continuous or pulsed laser light. Increased membrane permeability can be achieved directly by focused laser light or in combination with sensitizing nanoparticles for higher throughput. Here, we provide a detailed account on the history and current state-of-the-art of photoporation as a physical nanomaterial delivery technique. We first introduce with a detailed explanation of the mechanisms responsible for cell membrane pore formation, following an overview of experimental procedures for realizing direct laser photoporation. Next, we review the second and most recent method of photoporation that combines laser light with sensitizing NPs. The different mechanisms of pore formation are discussed and an overview is given of the various types of sensitizing nanomaterials. Typical experimental setups to achieve nanoparticle-mediated photoporation are discussed as well. Finally, we discuss the biological and therapeutic applications enabled by photoporation and give our current view on this expanding research field and the challenges and opportunities that remain for the near future.

Multilayered Magnetic Gelatin Membrane Scaffolds

A versatile approach for the design and fabrication of multilayer magnetic scaffolds with tunable magnetic gradients is described. Multilayer magnetic gelatin membrane scaffolds with intrinsic magnetic gradients were designed to encapsulate magnetized bioagents under an externally applied magnetic field for use in magnetic-field-assisted tissue engineering. The temperature of the individual membranes increased up to 43.7 °C under an applied oscillating magnetic field for 70 s by magnetic hyperthermia, enabling the possibility of inducing a thermal gradient inside the final 3D multilayer magnetic scaffolds. On the basis of finite element method simulations, magnetic gelatin membranes with different concentrations of magnetic nanoparticles were assembled into 3D multilayered scaffolds. A magnetic-gradient-controlled distribution of magnetically labeled stem cells was demonstrated in vitro. This magnetic biomaterial-magnetic cell strategy can be expanded to a number of different magnetic biomaterials for various tissue engineering applications.

Curing kinetics of step-index and graded-index single mode polymer self-written waveguides

A low-loss polymer medium to interconnect 2 single mode optical fibers is developed and characterized. It consists of a so-called self-written waveguide (SWW) formed by illuminating a photosensitive polymerization mix with light emanating from the fiber, after which the exposed part polymerizes. Depending on the material system used, this waveguide can have a step index or graded refractive index profile. The fabrication process and its effect on the waveguide performance are explained using an empirical model and afterwards experimentally verified. This approach enables easy process monitoring and optimization, effectively resulting in total insertion losses below 0.3 dB for a single mode fiber-SWW-fiber transition at 1550 nm.

High-resolution synchrotron X-ray analysis of bioglass-enriched hydrogels

Enrichment of hydrogels with inorganic particles improves their suitability for bone regeneration by enhancing their mechanical properties, mineralizability, and bioactivity as well as adhesion, proliferation, and differentiation of bone-forming cells, while maintaining injectability. Low aggregation and homogeneous distribution maximize particle surface area, promoting mineralization, cell-particle interactions, and homogenous tissue regeneration. Hence, determination of the size and distribution of particles/particle agglomerates in the hydrogel is desirable. Commonly used techniques have drawbacks. High-resolution techniques (e.g., SEM) require drying. Distribution in the dry state is not representative of the wet state. Techniques in the wet state (histology, µCT) are of lower resolution. Here, self-gelling, injectable composites of Gellan Gum (GG) hydrogel and two different types of sol-gel-derived bioactive glass (bioglass) particles were analyzed in the wet state using Synchrotron X-ray radiation, enabling high-resolution determination of particle size and spatial distribution. The lower detection limit volume was 9 × 10(-5) mm(3) . Bioglass particle suspensions were also studied using zeta potential measurements and Coulter analysis. Aggregation of bioglass particles in the GG hydrogels occurred and aggregate distribution was inhomogeneous. Bioglass promoted attachment of rat mesenchymal stem cells (rMSC) and mineralization.