Sara Maria Moreira Lima Verde

Label-Free Proteome Analysis of Plasma from Patients with Breast Cancer: Stage-Specific Protein Expression

Breast cancer is one of the most commonly diagnosed types of cancer among women. Breast cancer mortality rates remain high probably because its diagnosis is hampered by inaccurate detection methods. Since changes in protein expression as well as modifications in protein glycosylation have been frequently reported in cancer development, the aim of this work was to study the differential expression as well as modifications of glycosylation of proteins from plasma of women with breast cancer at different stages of disease (n = 30) compared to healthy women (n = 10). A proteomics approach was used that depleted albumin and IgG from plasma followed by glycoprotein enrichment using immobilized Moraceae lectin (frutalin)-affinity chromatography and data-independent label-free mass spectrometric analysis. Data are available via ProteomeXchange with identifier PXD003106. As result, 57,016 peptides and 4,175 proteins among all samples were identified. From this, 40 proteins present in unbound (PI—proteins that did not interact with lectin) and bound (PII—proteins that interacted with lectin) fractions were differentially expressed. High levels of apolipoprotein A-II were detected here that were elevated significantly in the early and advanced stages of the disease. Apolipoprotein C-III was detected in both fractions, and its level was increased slightly in the PI fraction of patients with early-stage breast cancer and expressed at higher levels in the PII fraction of patients with early and intermediate stages. Clusterin was present at higher levels in both fractions of patients with early and intermediate stages of breast cancer. Our findings reveal a correlation between alterations in protein glycosylation, lipid metabolism, and the progression of breast cancer.

Padrão alimentar de mulheres com câncer de mama: um estudo a posteriori

Objective: To identify a posteriori dietary pattern of women with breast cancer. Methods: Observational, cross-sectional and analytical study. It evaluated 100 women with breast cancer, undergoing chemotherapy/radiotherapy, treated at a cancer center in the city of Fortaleza, from July to December 2012. The study collected clinical data (subtype, clinical stage - CS, treatment), socioeconomic (educational level and monthly income) and anthropometric data (weight, height, body mass index - BMI), and food consumption. To evaluate the consumption, a quantitative food frequency questionnaire (FFQ), validated for women of the Brazilian Northeast, was applied, and the daily consumption of each food was set in grams/ml, being grouped according to their nutritional composition, into 10 groups, following the food pyramid adapted to the Brazilian population. The dietary pattern was defined by food components from the exploratory factor analysis of those ten food groups, using the SPSS, version 20.0. Results: Mean age of 50.9±10.2 years, with prevalence of ductal carcinoma (n=83; 83%) in CS III (n=60; 60%). The mean BMI indicated overweight (28.3±4.4 kg/m2) and waist circumference was above the recommended (98.6±10.9 cm). Four food components were identified, and 21% of the diet variance was explained by food component 1 (Risk), with the matrix composed of red and processed meat, oils, fats and cereals. Conclusion: The patients’ dietary pattern was characterized by a risk diet, which can positively contribute to the disease recurrence. doi: 10.5020/18061230.2014.p365

Conhecimento nutricional de mulheres com câncer de mama e sua relação com o estado nutricional - doi:10.5020/18061230.2013.p71

Objective: To assess the nutritional knowledge of women with breast cancer on the dietdisease interface and its association with nutritional status. Methods: Observational, crosssectional and analytical study, conducted between June and September 2011, with 59 women diagnosed with breast cancer, undergoing chemotherapy or radiotherapy treatment, older than 19, who did not receive prior nutritional counseling. Vegetarian women or those whose treatment had been completed more than two years prior to the study were not included. The patients were treated at a cancer care reference center, in Fortaleza-CE. Clinical and socioeconomic data was collected through direct interview and searching in medical records. The assessment of nutritional knowledge (NK) was performed with the Nutrition Knowledge Scale, developed by the National Health Interview Survey Cancer Epidemiology, validated for Brazil, applied by a trained interviewer. Nutritional status was assessed through body mass index (BMI) and waist circumference. Data was analyzed statistically by SPSS 16.0. Results: Among 59 patients evaluated, 18 (30.5%) women had a limited knowledge of the diet-disease association. The mean BMI was 29 kg/m2 (± 4.4) and 47 (79.7%) women presented excessive weight (overweight or obesity). There was no correlation between nutritional knowledge and BMI (p = 0.64). Nutrition knowledge scores were similar among patients with overweight and normal weight (p = 0.89). Conclusion: Women in this study had a limited knowledge of the interface between diet and disease, were overweight, but there was no correlation between their nutritional knowledge and nutritional status.