Sarah Brandão Santa Cruz Barboza

Anatomia foliar de plantas micropropagadas de abacaxi

The objective of this work was to study leaf anatomy of pineapple plants in order to improve acclimatization protocols. In vitro plantlets weighting an average of 3.1 g and greenhouse plantlets derived from in vitro stock material, after six and ten months of culture, weighting an average of 50.2 and 65 g, respectively, were used. Stomatal density was determined on the abaxial epidermis, at the basal, median, and apical portions of the leaf, using a completely randomized design under 2x3 factorial (two culture environments and three leaf regions) with six replicates. Thickness of the hypodermis, aquiferous and photosynthetic parenchyma were determined at the median portion of the leaf using a totally randomized design with three treatments and four replicates. The basic structure of the pineapple leaf under in vitro conditions did not change. However, stomatal frequency, cuticular and epidermal wall thickening, shape and sinuosity of the cell walls of aquiferous parenchyma and the presence of papillary cells were verified as a result of environmental conditions during culture, indicating phenotypic plasticity.The objective of this work was to study leaf anatomy of pineapple plants in order to improve acclimatization protocols. In vitro plantlets weighting an average of 3.1 g and greenhouse plantlets derived from in vitro stock material, after six and ten months of culture, weighting an average of 50.2 and 65 g, respectively, were used. Stomatal density was determined on the abaxial epidermis, at the basal, median, and apical portions of the leaf, using a completely randomized design under 2x3 factorial (two culture environments and three leaf regions) with six replicates. Thickness of the hypodermis, aquiferous and photosynthetic parenchyma were determined at the median portion of the leaf using a totally randomized design with three treatments and four replicates. The basic structure of the pineapple leaf under in vitro conditions did not change. However, stomatal frequency, cuticular and epidermal wall thickening, shape and sinuosity of the cell walls of aquiferous parenchyma and the presence of papillary cells were verified as a result of environmental conditions during culture, indicating phenotypic plasticity.

Crescimento inicial de mangabeira (Hancornia speciosa Gomes) em diferentes meios de germinação in vitro

O objetivo do trabalho foi determinar as condicoes mais favoraveis para a germinacao in vitro de sementes e o crescimento inicial de plântulas de mangabeira. Apos assepsia, sementes oriundas de frutos maduros foram inoculadas em tubos de ensaio contendo os seguintes tratamentos: T1-15 mL de meio de cultura MS; T2-15 mL de meio de cultura MS + 2,0 g L-1 de carvao ativado; T3-15 mL de meio de cultura ½ MS; e T4-15 mL de meio de cultura ½ MS + 2,0 g L-1 de carvao ativado. Todos os meios de cultura foram gelificados com 0,3 g L-1 de Phytagel® e suplementados com 3,0 g L-1 de sacarose. O experimento foi instalado em delineamento inteiramente casualizado com quatro tratamentos e oito repeticoes, sendo cada parcela experimental composta de dez tubos de ensaio contendo uma semente cada. Nao houve diferenca significativa dos tratamentos para a porcentagem de germinacao aos 20 dias, que variou de 95 a 100%. Quanto ao comprimento da raiz principal, observou-se que o meio de cultura constituido de ½ MS com 2,0 g L-1 de carvao ativado proporcionou maior crescimento quando comparado com os demais tratamentos. Aos 50 dias, nao foi observada a formacao de plântulas anormais e nem diferencas significativas do comprimento da parte aerea das plântulas. Entretanto, a diluicao em 50% dos sais do meio MS associada a presenca de carvao ativado induziu maior crescimento da raiz principal (8,50 cm) quando comparado com meio MS, na presenca (6,19 cm) ou ausencia (6,00 cm) de carvao ativado.

Estiolamento e regeneração na multiplicação in vitro do abacaxizeiro híbrido PE x SC-52

receberam inoculo em tubos de ensaio contendo o meio de cultura MS e mantidosno escuro por 60 dias, para estiolamento. Foram utilizados trŒs tratamentos, sem reguladores de cresci-mento, ANA a 1,86€mg/L e AIA a 1,75€mg/L em cinco repeticies com dez explantes por repeticao. Naohouve diferenca no nœmero de brotos estiolados por explante entre os tratamentos avaliados. No€entanto,aos 35 dias de cultivo, os tratamentos com ANA e AIA apresentavam maior nœmero de nos por broto,sendo o ANA superior aos demais apos 60 dias. Brotos estiolados