Sarawut Kaewsri

Standardized karyotype of the three-spot gourami, Trichogaster trichopterus (Perciformes, Belontidae) from Thailand by conventional and Ag-NOR staining technique

Standardized karyotype and idiogram of the three-spot gourami (Trichogaster trichopterus) from local market in Roi-Et province, northeast Thailand, were studied. Kidney cell samples were taken from ten male and ten female fishes. The mitotic chromosome preparations were prepared by blood cell culture as well as directly from kidney cells. Conventional and Ag-NOR staining techniques were applied to stain the chromosomes. The results showed that diploid chromosome number of T. trichopterus was 2n = 46, the fundamental numbers (NF) were 46 in both male and female. The types of autosomes were 12 large telocentric, 32 medium telocentric and 2 small telocentric chromosomes. The region adjacent to the telomere of chromosome pair 2 showed clearly observable secondary constriction / NORs. The karyotype formula for T. trichopterus could be deduced as:

A Discovery of Polymorphism of Nucleolar Organizer Regions (NORs) and Whole-Arm Translocation (WAT) between Chromosome 8 and 9 of Lowland Agile Gibbon (Hylobates agilis unko) in Thailand

{2}n\left( {\hbox{diploid}} \right){46} = {\hbox{L}}_{{12}}^{\rm{t}} + {\hbox{M}}_{{32}}^{\rm{t}} + {\hbox{S}}_2^{\rm{t}}

The Identification of the Sex Chromosome and Karyotype of Four Toad Species (Genus Bufo) in Thailand by T-lymphocyte Cell Culture

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Cytogenetics of the Skinks (Reptilia, Scincidae) from Thailand; I: Chromosome Analyses of the Common Sun Skink (Eutropis multifasciata)

The first cytogenetic comparisons of five snapper species from Thailand were presented here. Renal cell samples were taken from blacktail snapper (Lutjanus fulvus), five lined snapper (L. quinquelineatus), dory snapper (L. fulviflamma), brownstripe red snapper (L. vitta), and mangrove red snapper (L. argentimaculatus). The mitotic chromosome preparation was prepared directly from kidney cells. Conventional staining and Ag-NOR banding techniques were applied to stain the chromosomes. The results exhibited that all five snapper species have the diploid chromosome numbers of 2n = 48 and the fundamental numbers (NF) of 48. The presences of large, medium, and small telocentric chromosomes were 22-24-2, 24-20-4, 36-10-2, 28-16-4 and 36-10-2, respectively. The Ag- NORs banding technique provides the pair of nucleolar organizer regions (NORs) at subcentromeric region of the long arm of the respective telocentric chromosome pairs 9, 1, 3, 4 and 9. Their karyotype formulas is as follows: L. fulvus (2n = 48): L22t + M24t + S2t, L. quinquelineatus (2n = 48): L24t + M20t + S4t, L. fulviflamma (2n = 48): Lt36 + Mt10 + St2, L. vitta (2n = 48): L28t + M16t + S4t, and L. argentimaculatus (2n = 48): L36t + M10t + S2t.