Sardar Khan

Sewage Sludge Biochar Influence upon Rice (Oryza sativa L) Yield, Metal Bioaccumulation and Greenhouse Gas Emissions from Acidic Paddy Soil

Biochar addition to soil has been proposed to improve plant growth by increasing soil fertility, minimizing bioaccumulation of toxic metal(liod)s and mitigating climate change. Sewage sludge (SS) is an attractive, though potentially problematic, feedstock of biochar. It is attractive because of its large abundance; however, it contains elevated concentrations of metal(loid)s and other contaminants. The pyrolysis of SS to biochar (SSBC) may be a way to reduce the availability of these contaminants to the soil and plants. Using rice plant pot experiments, we investigated the influence of SSBC upon biomass yield, bioaccumulation of nutrients, and metal(loid)s, and green housegas (GHG) emissions. SSBC amendments increased soil pH, total nitrogen, soil organic carbon and available nutrients and decreased bioavailable As, Cr, Co, Ni, and Pb (but not Cd, Cu, and Zn). Regarding rice plant properties, SSBC amendments significantly (P ≤ 0.01) increased shoot biomass (71.3-92.2%), grain yield (148.8-175.1%), and the bioaccumulation of phosphorus and sodium, though decreased the bioaccumulation of nitrogen (except in grain) and potassium. Amendments of SSBC significantly (P ≤ 0.05) reduced the bioaccumulation of As, Cr, Co, Cu, Ni, and Pb, but increased that of Cd and Zn, though not above limits set by Chinese regulations. Finally regarding GHG emissions, SSBC significantly (P < 0.01) reduced N2O emissions and stimulated the uptake/oxidation of CH4 enough to make both the cultivated and uncultivated paddy soil a CH4 sink. SSBC can be beneficial in rice paddy soil but the actual associated benefits will depend on site-specific conditions and source of SS; long-term effects remain a further unknown.

Soil and vegetables enrichment with heavy metals from geological sources in Gilgit, northern Pakistan.

This study was conducted to investigate the concentrations of heavy metals in soil and vegetables, and human health risks through ingestion of contaminated vegetables. Soil and vegetable samples were collected from different locations in Gilgit, northern Pakistan, and analyzed for Cd, Cu, Ni, Pb and Zn. Plant transfer factors (PTF), daily intake of metals (DIM) and health risk index (HRI) were also calculated. The concentrations of Cd, Cu and Zn exceeded their respective permissible limits in soil samples. The highest concentrations of Cu, Ni, Pb and Zn were observed in the edible parts of Malva neglecta, Brassica oleracea, Mintha sylvestris and Brassica campestris, respectively. PTF values were lower for all the selected heavy metals, except for Cd. Furthermore, the HRI values were within the safe limit (<1) except for Pb; therefore, the health risks of metals through ingestion of vegetables were of great concern in the study area.

Use of constructed wetland for the removal of heavy metals from industrial wastewater

This study was conducted to investigate the effectiveness of a continuous free surface flow wetland for removal of heavy metals from industrial wastewater, in Gadoon Amazai Industrial Estate (GAIE), Swabi, Pakistan. Industrial wastewater samples were collected from the in-let, out-let and all cells of the constructed wetland (CW) and analyzed for heavy metals such as lead (Pb), cadmium (Cd), iron (Fe), nickel (Ni), chromium (Cr) and copper (Cu) using standard methods. Similarly, samples of aquatic macrophytes and sediments were also analyzed for selected heavy metals. Results indicate that the removal efficiencies of the CW for Pb, Cd, Fe, Ni, Cr, and Cu were 50%, 91.9%, 74.1%, 40.9%, 89%, and 48.3%, respectively. Furthermore, the performance of the CW was efficient enough to remove the heavy metals, particularly Cd, Fe, and Cu, from the industrial wastewater fed to it. However, it is suggested that the metal removal efficiency of the CW can be further enhanced by using proper management of vegetation and area expansion of the present CW.

Soil enzymatic activities and microbial community structure with different application rates of Cd and Pb

This study focused on the changes of soil microbial diversity and potential inhibitory effects of heavy metals on soil enzymatic activities at different application rates of Cd and/or Pb. The soil used for experiments was collected from Beijing and classified as endoaquepts. Pots containing 500 g of the soil with different Cd and/or Pb application rates were incubated for a period of 0, 2, 9, 12 weeks in a glasshouse and the soil samples were analyzed for individual enzymes, including catalase, alkaline phosphatase and dehydrogenase, and the changes of microbial community structure. Results showed that heavy metals slightly inhibited the enzymatic activities in all the samples spiked with heavy metals. The extent of inhibition increased significantly with increasing level of heavy metals, and varied with the incubation periods. The soil bacterial community structure, as determined by polymerase chain reaction-denaturing gradient gel electrophoresis techniques, was different in the contaminated samples as compared to the control. The highest community change was observed in the samples amended with high level of Cd. Positive correlations were observed among the three enzymatic activities, but negative correlations were found between the amounts of the heavy metals and the enzymatic activities.

A comparative study of human health risks via consumption of food crops grown on wastewater irrigated soil (Peshawar) and relatively clean water irrigated soil (lower Dir)

Food crops irrigated with wastewater are mostly contaminated with heavy metals and considered as a main pathway for human exposure. In this study, soil and food crops samples were collected from wastewater irrigated soils, background and relatively less polluted areas. Results of the sequential extraction and total metals concentrations in soils indicated that wastewater irrigation has significantly increased (p > or = 0.001) the bioavailable and total metal contents in wastewater irrigated soil as compared to background and control soils. Heavy metal concentrations in the food crops grown on wastewater irrigated soil were higher than those grown on background and control soils but were found within WHO/FAO permissible limits except for Zn. Health risk index values were less than 1 for both control and wastewater irrigated soils (except Mn). However, the food crops such as Brassica rapa, Spinacia oleracae L., Lycopersicum esculantum, Mentha viridis, Coriandum sativum and Lactuca sativa grown on wastewater irrigated soil can pose health risks because of the high concentration of Mn.

Arsenic health risk assessment in drinking water and source apportionment using multivariate statistical techniques in Kohistan region, northern Pakistan.

The present study was conducted in Kohistan region, where mafic and ultramafic rocks (Kohistan island arc and Indus suture zone) and metasedimentary rocks (Indian plate) are exposed. Water samples were collected from the springs, streams and Indus river and analyzed for physical parameters, anions, cations and arsenic (As(3+), As(5+) and arsenic total). The water quality in Kohistan region was evaluated by comparing the physio-chemical parameters with permissible limits set by Pakistan environmental protection agency and world health organization. Most of the studied parameters were found within their respective permissible limits. However in some samples, the iron and arsenic concentrations exceeded their permissible limits. For health risk assessment of arsenic, the average daily dose, hazards quotient (HQ) and cancer risk were calculated by using statistical formulas. The values of HQ were found >1 in the samples collected from Jabba, Dubair, while HQ values were <1 in rest of the samples. This level of contamination should have low chronic risk and medium cancer risk when compared with US EPA guidelines. Furthermore, the inter-dependence of physio-chemical parameters and pollution load was also calculated by using multivariate statistical techniques like one-way ANOVA, correlation analysis, regression analysis, cluster analysis and principle component analysis.

The influence of various biochars on the bioaccessibility and bioaccumulation of PAHs and potentially toxic elements to turnips (Brassica rapa L.)

The influence of amending a contaminated soil with different dry-pyrolyzed biochars on the bioaccessibility and biouptake of polycyclic aromatic hydrocarbons (PAH) and potentially toxic elements (PTE) in turnip (Brassica rapa L.,) was investigated. This is the first study to examine the influence of biochar amendments on turnips grown in a contaminated soil. The biochars came from different local feedstocks, including sewage sludge biochar (SSBC), soybean straw biochar (SBBC), rice straw biochar (RSBC) and peanut shell biochar (PNBC). The biochars were applied to soil at 2% and 5% amendments, and the resulting influence on various soil and porewater properties were quantified. The bioaccessible concentrations of PAHs in soil and their bioaccumulation in B. rapa L. significantly (P < 0.05) decreased in the biochar amended soils. Biochar additions significantly (P ≤ 0.05) reduced the bioaccumulation of PTEs (As, Cd, Cu, Pb and Zn) in B. rapa L, though not as much as for PAHs. The most effective biochar at reducing both PAHs and PTEs was PNBC (P ≤ 0.05). Amendments of 5% biochar were more effective at reducing contaminant bioaccessibility than amendments at 2% (P < 0.05). Crop yield, however, increased the most for the 2% biochar amendments, in particular for SSBC (with a 49% increase in crop yield compared to the non-amended soil). Therefore, which biochar would be the most advantageous in this system would require a cost-benefit analysis between increasing crop yield (best achieved with 2% SSBC amendments) and decreasing the PAH and PTE uptake (best achieved with 5% PNBC amendments).

The uptake and bioaccumulation of heavy metals by food plants, their effects on plants nutrients, and associated health risk: a review

Heavy metal contamination is a globally recognized environmental issue, threatening human life very seriously. Increasing population and high demand for food resulted in release of various contaminants into environment that finally contaminate the food chain. Edible plants are the major source of diet, and their contamination with toxic metals may result in catastrophic health hazards. Heavy metals affect the human health directly and/or indirectly; one of the indirect effects is the change in plant nutritional values. Previously, a number of review papers have been published on different aspects of heavy metal contamination. However, no related information is available about the effects of heavy metals on the nutritional status of food plants. This review paper is focused upon heavy metal sources, accumulation, transfer, health risk, and effects on protein, amino acids, carbohydrates, fats, and vitamins in plants. The literature about heavy metals in food plants shows that both leafy and nonleafy vegetables are good accumulators of heavy metals. In nonleafy vegetables, the bioaccumulation pattern was leaf > root ≈ stem > tuber. Heavy metals have strong influence on nutritional values; therefore, plants grown on metal-contaminated soil were nutrient deficient and consumption of such vegetables may lead to nutritional deficiency in the population particularly living in developing countries which are already facing the malnutrition problems.

The effects of sewage sludge and sewage sludge biochar on PAHs and potentially toxic element bioaccumulation in Cucumis sativa L.

The presence of contaminants such as polycyclic aromatic hydrocarbons (PAHs) and potentially toxic elements (PTEs), including As, Cd, Cu, Pb and Zn, restricts the application of sewage sludge (SS) to agricultural land. This research established that the conversion of SS to SS biochar (SSBC) significantly (p ≤ 0.01) decreased PAH and available PTE concentrations. Once added to soil both SS and SSBC significantly (p ≤ 0.05) decrease PAH availability. Bioaccumulation of PAHs into Cucumis sativa L. was reduced by both SSBC (44-57%) and (to a lesser extent 20-36%) by SS. Following addition to soil SSBC significantly (p ≤ 0.05) reduced available PTEs (except Cd), while SS significantly (p ≤ 0.05) increased PTE availability. As a consequence SSBC significantly (p ≤ 0.05) reduced PTE bioaccumulation (except Cd and Zn), while SS increased PTE bioaccumulation. These results suggest SSBC to be a candidate for soil amendment that offers advantages over SS in terms of PAH/PTE bioaccumulation mitigation.

Heavy metals in agricultural soils and crops and their health risks in Swat District, northern Pakistan.

This study assessed the concentrations of heavy metals such as cadmium (Cd), chromium (Cr), copper (Cu), manganese (Mn), nickel (Ni) and zinc (Zn) in agricultural soils and crops (fruits, grains and vegetable) and their possible human health risk in Swat District, northern Pakistan. Cd concentration was found higher than the limit (0.05 mg/kg) set by world health organization in 95% fruit and 100% vegetable samples. Moreover, the concentrations of Cr, Cu, Mn, Ni and Zn in the soils were shown significant correlations with those in the crops. The metal transfer factor (MTF) was found highest for Cd followed by Cr>Ni>Zn>Cu>Mn, while the health risk assessment revealed that there was no health risk for most of the heavy metals except Cd, which showed a high level of health risk index (HRI⩾10E-1) that would pose a potential health risk to the consumers.