Sati C. Chattoraj

A radioimmunoassay method for urinary catechol estrogens

A radioimmunoassay method for urinary catechol estrogens is described; The specific nature of the antisera allows direct analyses of acid hydrolyzed urine. A LH-20 Sephadex column chromatography can be employed for individual determinations of 2-hydroxyestrone and 2-hydroxyestradiol. The excretion of catechol estrogens during menstrual cycles ranged from 14.48 to 50.15 microgram per 24 hours, whereas, during the last trimester of pregnancies, the values ranged from 129.30 to 1758. 20 microgram per 24 hours.

The significance of urinary free cortisol and progesterone in normal and anencephalic pregnancy.

Urinary free cortisol and progesterone were determined by radioimmunoassay in 18 normal and 16 anencephalic pregnancies and urinary free cortisol levels in 9 nonpregnant women. In normal pregnancy the urinary free cortisol (46.89 +/- 34.02 mug per 24 hours) was significantly higher (P less than 0.001) than that found with anencephaly (17.19 +/- 13.20 mug per 24 hours) and 2 1/2 times (P less than 0.001) the nonpregnant value (18.47 +/- 5.44 mug per 24 hours). In 12 of the anencephalic pregnancies, urinary free cortisol levels (11.05 +/- 5.56 mug per 24 hours) were significantly lower than in nonpregnant women (P less than 0.001). Urinary progesterone levels in normal pregnancy (15.57 +/- 9.66 mug per 24 hours) and anencephaly (18.54 +/- 12.69 mug per 24 hours) were comparable. The cortisol excretion values associated with anencephaly indicate that the normal fetus contributes substantially to the maternal plasma cortisol pool. Urinary free cortisol determinations may be a useful index of fetal adrenal dysfunction.

Studies in steroid metabolism XX. “The determination of plasma testosterone using thin-layer and gas-liquid chromatography.”

Abstract A method has been devised for the relatively rapid and sensitive determination of testosterone in human plasma. The procedure incorporates a thin-layer chromatogram to separate most of the 17-ketosteroids from testosterone followed by GLC on silicone elastomer. An average recovery of 39% of added 3H-testosterone was obtained, Values for young males of 0.30–1.27 μg 100 ml of plasma were found.

Quantitative extraction of steroid conjugates from urine by use of a liquid-liquid partition system

The present communication describes a method for the quantitative recovery of glucuronic and sulfuric acid conjugated urinary steroids.

The effect of dexamethasone on the urinary excretion of steroids during pregnancy.

The urinary excretion of oestriol, total 17‐oxosteroids, pregnanediol and Pettenkofer chromogens were studied in eight pregnancies before, during and after the administration of dexamethasone. Both the oestriol and 17‐oxosteroid levels declined during the period of drug administration. The change in Pettenkofer chromogens was less consistent but generally paralleled the 17‐oxosteroid values. Pregnanediol excretion greatly increased in three subjects, decreased in one and was relatively unchanged in the other patients. The present studies support the concept that placental pregnenolone is utilized by the fetal tissues for the biosynthesis of oestrogen precursors and by the placenta for progesterone synthesis.

A gas chromatographic technique for the simultaneous determination of urinary pregnanediol and pregnanetriol after ammonium sulfate precipitation

Abstract A gas Chromatographic technique for simultaneous determination of urinary pregnanediol and pregnanetriol is described. The method involves precipitation of free steroids as well as conjugates of steroids by 70 per cent ammonium sulfate from the urine, enzyme hydrolysis, extraction, acetylation and injection into a gas Chromatograph. The usefulness of the precipitation technique is validated and discussed. The data on reliability criteria, such as accuracy, precision, specificity, sensitivity and applicability to the urine samples are documented.

Temporal relationships among the excretory patterns of 2-hydroxyestrone, estrone, estradiol, and progesterone during pregnancy in the rat

The urinary excretion pattern of 2-hydroxyestrone, estradiol, estrone, and progesterone was examined in rats during early, mid, and late pregnancy. Progesterone increased from early to mid pregnancy and declined significantly 2 to 3 days prior to parturition, corresponding to changes observed in blood levels by others. 2-Hydroxyestrone, the major estrogen in rat urine, increased significantly 4 days prior to delivery and remained elevated until it further increased sharply the day of parturition. Urinary estradiol and estrone levels showed little change until the day of parturition, when they increased significantly. Multiple correlation analysis of the data implied that 2-hydroxyestrone and estradiol were negatively correlated at the time of implantation. The results suggest that catechol estrogens, through their effect on prostaglandin synthesis, may participate in the process of implantation as well as in the mechanism involved in the onset of labor.

Deuterium-Labeled Steroids for Study in Humans

6,7-Dideuterio-3-hydroxy-1,3,5(10)-estratrien-17-one (dideuterio-estrone) and 4-deuterio-1,3,5(10)-estratriene-3,17 β -diol (monodeuterio-17 β -estradiol) were used f

Do catechol estrogens participate in the initiation of labor

To assess the role of catechol estrogens in the initiation of labor, we compared the levels in amniotic fluid during the second and third trimesters and from women undergoing cesarean section at term not in labor and those with spontaneous labor at term. Catechol estrogen concentrations in amniotic fluid increased significantly with the progress of pregnancy. Further, concentrations (mean +/- SE) were significantly higher in spontaneous labor at term (468.6 +/- 29.5 pg/ml) compared with those obtained during cesarean section (242.6 +/- 22.3 pg/ml) at term not in labor. We suggest that catechol estrogens, through their stimulating effects on prostaglandin synthesis, participate in the initiation of labor.

Effect of pregnenolone sulfate administration on the excretion of steroid hormones in pregnant women

Abstract The urinary excretion of pregnanediol, estriol, total 17-ketosteroids and Pettenkofer chromogens were studied after the intravenous administration of 100 or 200 mg of pregnenolone sulfate to pregnant women. The maximum excretion values were observed for 17-ketosteroids and Pettenkofer chromogens within 24 hours. Estriol excretion also showed a substantial rise within 72 hours. The excretion pattern of pregnanediol mirrored that of estriol but with minimum initial rise.