Satoshi Hashimoto

Induction of sister-chromatid exchanges in Chinese hamster V79 cells by exposure to the photochemical reaction products of toluene plus NO2 in the gas phase

A study was made on the induction of sister-chromatid exchanges (SCE) in cultured Chinese hamster V79 cells exposed to the photochemical reaction products of toluene plus NO2 in the gas phase. The photochemical reaction products of toluene plus NO2 were obtained by photochemical reaction of a toluene--NO2/dry air system in a photochemical smog chamber and then exposed to cultured cells for 2 h using a system for in vitro gas exposure. SCEs were induced at all concentrations of the photochemical reaction products employed in the present study, and the highest SCE frequency observed for the highest concentration tested for each component was 3.6 times higher than that of the control. Cytogenotoxicity which was evaluated with induced-SCEs of the photochemical reaction products of toluene plus NO2 was much the same as that of the previously reported photochemical reaction products of propylene plus NO2 (Shiraishi and Bandow, 1985), but was considerably stronger than that of typical gaseous air pollutants such as NO2 alone and O3 alone.

Infrared spectroscopic detection of the HOSO2 radical in argon matrix at 11 k

Abstract The IR absorption spectrum of hydroxysulfonyl (HOSO 2 ) radical, an intermediate of atmospheric oxidation of SO 2 , has been obtained in argon matrix at 11 K in the reaction of SO 2 and OH prepared by the photolysis of H 2 O 2 or H 2 O during deposition. The observed vibrational frequencies are 3539.9, 1309.2, 1097.3 and 759.5 cm −1 . The IR absorption bands Of H 18 OSO 2 were also obtained by using H 2 18 O as a hydroxyl radical source.