Satoshi Koyama

1H-NMR analysis of the sugar structures of glycoproteins as their pyridylamino derivatives

The 1H‐NMR spectra of a series of pyridylamino (PA‐) derivatives of oligosaccharides were obtained and compared with those of the corresponding asparagine‐linked sugar chains in order to elucidate the effect of the PA‐group on the chemical shifts of structural‐reporter signals. The effects were found to be localized within the two residues from the end group. Thus, the data for asparagine‐linked chains in the literature are applicable to PA‐derivatives, so the combination of pyridylamination and NMR measurements greatly reduces the time required for structure analysis of sugar chains of glycoproteins, because the isolation and purification of the chains as PA‐derivatives are easy and efficient.