Satoshi Ohashi

Analyses of Mitogen-Activated Protein Kinase Function in the Maturation of Porcine Oocytes

Abstract The function of mitogen-activated protein kinase (MAPK) during porcine oocyte maturation was examined by injecting oocytes with either mRNA or antisense RNA of porcine c-mos protein, an upstream kinase of MAPK. The RNAs were injected into the cytoplasm of porcine immature oocytes immediately after collection from ovaries, then the oocytes were cultured for maturation up to 48 h. The phosphorylation and activation of MAPK were observed at 6 h after injection of the c-mos mRNA injected-oocytes, whereas in control oocytes, MAPK activation was detected at 24 h of culture. The germinal vesicle breakdown (GVBD) rate at 24 h of culture was significantly higher in c-mos mRNA-injected oocytes than in control oocytes. In contrast, although injection of c-mos antisense RNA completely inhibited phosphorylation and activation of MAPK throughout the maturation period, the GVBD rate and its time course were the same in noninjected oocytes. The degree of maturation-promoting factor (MPF) activation was, however, very low in oocytes in the absence of MAPK activation. Most of those oocytes had both abnormal morphology and decondensed chromosomes at 48 h of culture. These results suggest that MAPK activation is not required for GVBD induction in porcine oocytes and that the major roles of MAPK during porcine oocyte maturation are to promote GVBD by increasing MPF activity and to arrest oocytes at the second metaphase.

The TWO MOLECULAR CLOUDS IN RCW 38: EVIDENCE FOR THE FORMATION OF THE YOUNGEST SUPER STAR CLUSTER IN THE MILKY WAY TRIGGERED BY CLOUD–CLOUD COLLISION

We present distributions of two molecular clouds having velocities of 2 km s

Activation of ribosomal S6 kinase (RSK) during porcine oocyte maturation

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Dense Core Properties in the Infrared Dark Cloud G14.225-0.506 Revealed by Alma

and 14 km s

Chemical variation in molecular cloud cores in the Orion A cloud. II.

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Phosphorylation of mitogen-activated protein kinase cascade during early embryo development in the mouse

toward RCW 38, the youngest super star cluster in the Milky Way, in the

SPATIAL VARIATIONS OF PAH PROPERTIES IN M17SW REVEALED BY SPITZER/IRS SPECTRAL MAPPING

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Detection of a turbulent gas component associated with a starless core with subthermal turbulence in the Orion A cloud

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Precessing Jet and Large Dust Grains in the V380 Ori NE Star-forming Region

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Nobeyama 45m CO Galactic Plane Survey: Filament properties and star formation in M17

1--0 and 3--2) and