Scott A. Wight
National Institute of Standards and Technology
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Featured researches published by Scott A. Wight.
Journal of Orthopaedic Research | 2002
Carl G. Simon; C A. Khatri; Scott A. Wight; Francis W. Wang
We have assessed the biocompatibility of a new composite bone graft consisting of calcium phosphate cement (CPC) and poly(lactide‐co‐glycolide) (PLGA) microspheres (approximate diameter of 0.18–0.36 mm) using cell culture techniques. CPC powder is mixed with PLGA microspheres and water to yield a workable paste that could be sculpted to fit the contours of a wound. The cement then hardens into a matrix of hydroxyapatite microcrystals containing PLGA microspheres. The rationale for this design is that the microspheres will initially stabilize the graft but can then degrade to leave behind macropores for colonization by osteoblasts. The CPC matrix could then be resorbed and replaced with new bone. In the present study, osteoblast‐like cells (MC3T3‐E1 cells) were seeded onto graft specimens and evaluated with fluorescence microscopy, environmental scanning electron microscopy and the Wst‐1 assay (an enzymatic assay for mitochondrial dehydrogenase activity). Cells were able to adhere, attain a normal morphology, proliferate and remain viable when cultured on the new composite graft (CPC–PLGA) or on a control graft (CPC alone). These results suggest that our new cement consisting of CPC and PLGA microspheres is biocompatible. This is the first time that a ‘polymer‐in‐mineral’ (PLGA microspheres dispersed in a CPC matrix) cement has been formulated that is moldable, resorbable and that can form macropores after the cement has set.
Aerosol Science and Technology | 2003
Joseph M. Conny; Donna B. Klinedinst; Scott A. Wight; Jeffrey L. Paulsen
The chemical, physical, and morphological complexity of atmospheric aerosol elemental carbon (EC) presents major problems in assuring measurement accuracy. Since EC and black carbon are often considered equivalent, methods based on thermal-optical analysis (TOA) are widely used for EC in ambient air samples because no prior knowledge of the aerosols absorption coefficient is required. Nevertheless, different TOA thermal desorption protocols result in wide EC-to-total-carbon (TC) variation. We created three response surfaces with the following response variables: EC/TC, maximum laser attenuation in the He phase ( L max ), and laser attenuation at the end of the He phase ( L He4 ). A two-level central-composite factorial design comprised of four factors considered the temperatures and durations of all desorption steps in TOAs inert (He) phase and the initial step in TOAs oxidizing (O 2 -He) phase. L max was used to assess the positive bias caused by nonvolatile unpyrolized organic carbon (OC char) being measured as native EC. A negative bias that the attenuated laser response does not detect is caused by the loss of native EC in the He phase. L He4 was used as a surrogate indicator for the loss of native EC in the He phase. The intersection between the L max and L He4 surfaces revealed TOA conditions where both the production of OC char in the He phase was maximized and the loss of native EC in the He phase was minimized, therefore leading to an optimized thermal desorption protocol. Based on the sample types used in this study, the following are generalized optimal conditions when TOA is operated in the fixed-step-durations, laser-transmission mode (i.e., TOT): step 1 in He, 190°C for 60 s; step 2 in He, 365°C for 60 s; step 3 in He, 610°C for 60 s; step 4 in He, 835°C for 72 s. For steps 1-4 in O 2 -He, the conditions are 550°C for 180 s, 700°C for 60 s, 850°C for 60 s, and 900°C for 90 s to 120 s, respectively.
Applied Physics Letters | 1994
Greg Gillen; Scott A. Wight; J. Bennett; Michael J. Tarlov
Decanethiol [CH3(CH2)9SH] self‐assembled monolayer films on silver substrates have been irradiated in selected areas by focused ion or electron bombardment. Subsequent immersion of the irradiated sample in a solution of a fluoromercaptan [CF3(CF2)2(CH2)2SH] results in attachment of this molecule to the silver surface in the ion or electron‐exposed regions, producing a micrometer spatial‐scale pattern of two chemically distinct alkanethiol monolayers. The coverage of the fluoromercaptan on the bombarded areas was found to reach maximum levels of 70% at ion doses of 6×1013 ions/cm2 and 50% at electron doses of 2×1017 electrons/cm2 as determined by secondary ion mass spectrometry. These methods of maskless patterning may be useful for semiconductor or biosensor device fabrication.
Applied Physics Letters | 2004
N. Farkas; J. R. Comer; G. Zhang; Edward A. Evans; R. D. Ramsier; Scott A. Wight; John A. Dagata
Parallel pattern transfer of submicrometer-scale oxide features onto zirconium nitride thin films is reported. The oxidation reaction was verified by Auger microprobe analysis and secondary ion mass spectrometry. Oxide features of ∼70nm in height can be formed and selectively etched in a dilute aqueous hydrogen fluoride solution. This provides an interesting route to potential new applications for high-melting point, biocompatible surfaces that possess small feature sizes with controlled geometries.
Applied Radiation and Isotopes | 1998
Cynthia J. Zeissler; Scott A. Wight; Richard M. Lindstrom
Abstract Recent advances in instrumentation provide the capability to measure size, count rate, type of radiation, and the chemical composition of radioactive particles. We present a demonstration of these capabilities, using a laser-scanned phosphor imaging system and a charge injection device system for digital autoradiography, light and electron microscopy, energy dispersive X-ray spectrometry, and conventional radionuclide spectrometry. Response characteristics of the phosphor system that support these efforts are presented.
Drug Development and Industrial Pharmacy | 2014
Simeon K. Adesina; Scott A. Wight; Emmanuel O. Akala
Abstract Purpose: Nanoparticle size is important in drug delivery. Clearance of nanoparticles by cells of the reticuloendothelial system has been reported to increase with increase in particle size. Further, nanoparticles should be small enough to avoid lung or spleen filtering effects. Endocytosis and accumulation in tumor tissue by the enhanced permeability and retention effect are also processes that are influenced by particle size. We present the results of studies designed to optimize cross-linked biodegradable stealth polymeric nanoparticles fabricated by dispersion polymerization. Methods: Nanoparticles were fabricated using different amounts of macromonomer, initiators, crosslinking agent and stabilizer in a dioxane/DMSO/water solvent system. Confirmation of nanoparticle formation was by scanning electron microscopy (SEM). Particle size was measured by dynamic light scattering (DLS). D-optimal mixture statistical experimental design was used for the experimental runs, followed by model generation (Scheffe polynomial) and optimization with the aid of a computer software. Model verification was done by comparing particle size data of some suggested solutions to the predicted particle sizes. Results and conclusion: Data showed that average particle sizes follow the same trend as predicted by the model. Negative terms in the model corresponding to the cross-linking agent and stabilizer indicate the important factors for minimizing particle size.
Micron | 2012
Scott A. Wight; Andrew R. Konicek
Scattering of the incident electron beam in the variable pressure scanning electron microscope (VPSEM) affects the ability to perform quantitative chemical measurements. However, the manner in which the sum of the elastic and inelastic scattering cross sections varies as a function of gas type and accelerating voltage in the VPSEM is not well understood. A dual Faraday cup was constructed to measure the scattered fraction of the primary beam as a function of gas pressure, working distance, and accelerating voltage in air, water vapor, and argon environments. Experimentally measured values of the scattering cross section agree with previous experimental work, and agree within a factor of two with those values calculated carefully from theory.
MRS Proceedings | 1994
Kenneth G. Kreider; Donald R. Burgess; Michael J. Tarlov; Greg Gillen; Scott A. Wight; R. T. Lareau; L. M. Casas
Silicon carbide has excellent physical and electronic properties for use in devices when higher temperatures or higher power densities are required. We have investigated a direct laser conversion technique to create electrical conductors on the high band-gap silicon carbide. Thin films of silicon carbide (SiC) were sputter deposited on AI2O3, SiO2, and Si substrates using a SiC target with an RF planar magnetron. These films were irradiated at 308 nm with multiple 15 ns excimer laser pulses creating 0.5 to 2 mm wide electrically conducting paths. Both the irradiated and unirradiated films were evaluated as a function of substrate type, deposition temperature, finish, stoichiomelry, annealing temperature, sputter gas, film thickness, and laser processing conditions. The lowest resistivity films, originally 10 ohm-m, were calculated to be 160 μohm-m obtained after irradiation, which compares to a value of 50 μohm-m obtained after irradiating bulk SiC. The films were characterized using XPS, SIMS, AES, SEM, and Raman spectroscopy. We were able to characterize the composition of the films and conducting traces, the surface oxide, the critical binding energies, the lattice structure, and the morphology of the microstructure. Models for the phase transformations and conductivity have been formulated.
Fibers and Polymers | 2015
Walter G. McDonough; Joy P. Dunkers; Amanda L. Forster; N. Alan Heckert; Jae H. Kim; Scott A. Wight; Gale A. Holmes
Fibers containing 5-amino-2-(p-aminophenyl)benzimidazole are being considered for use as reinforcement in soft body armor applications. Past research in this laboratory has resulted in a suite of tests that have been used to detect degradation in other fibers and are now being applied to the fibers in question. Due to the architecture of the yarns in this study, two methods to extract single filaments for tensile testing were described and analyzed. A dry method resulted in fibers with surface damage, reflected in a high standard deviation in strength. A wet extraction method showed a reduction in surface damage and a lower standard deviation in strength. Fourier Transform Infrared analysis detected signs of hydrolysis in the fibers that were exposed to water. Although no noticeable loss in tensile strength was noted upon exposure, the possibility of hydrolysis in these fibers may indicate a need for further study.
Journal of Microscopy | 2005
Scott A. Wight; Richard D. Holbrook
A simplified method for the preparation of particles from liquid suspensions has been developed. Particles are deposited directly on carbon planchets for rapid analysis by environmental scanning electron microscopy or by conventional scanning electron microscopy after an additional drying step. This is accomplished by filtering the liquid through thin carbon planchets. Three different grades of graphite were investigated for their suitability as the source material for these planchets. The high quality isomolded graphite is recommended for the filtration and direct observation of particles by electron microscopy. This technique is demonstrated for particles in hydraulic fluid and aquatic suspended particulate material from a natural water source.