Se Ryun Kwon
Pukyong National University
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Featured researches published by Se Ryun Kwon.
Diseases of Aquatic Organisms | 2008
Dong Jin Lee; Se Ryun Kwon; Kosuke Zenke; Eun Hye Lee; Yoon Kwon Nam; Sung Koo Kim; Ki Hong Kim
A dual vector expressing the ghost-inducing PhiX174 lysis E gene and the bacterial DNA degrading staphylococcal nuclease A (SNA) gene was constructed to solve the problem of remnant antibiotic resistance genes and genomic DNA with intact pathogenic islands in the final product of Edwardsiella tarda ghosts (ETG). The SNA (devoid of secretion signal sequence and the nuclease B amino terminus sequence), fused with the 26 amino acid N-terminal sequence of the lambda phage Cro gene, showed successful degradation of bacterial nucleic acids. Furthermore, the nuclease activity of SNA in E. tarda was enhanced by codon optimization of the SNA gene using site-directed mutagenesis. ETG were generated via coexpression of the SNA gene and lysis gene E under the control of each lambdaP(R) promoter. The ghost bacteria generation system we describe is advantageous as it allows the use of a single plasmid, improves safety and vaccine purity by limiting residual genetic content from the ghost bacteria, and reduces production costs through cheap means of induction that use only temperature shifts.
Diseases of Aquatic Organisms | 2013
Hyoung Jun Kim; Se Ryun Kwon
The geographic distribution of koi herpesvirus (KHV) has recently been analyzed by polymerase chain reaction (PCR, based on the alleles of 3 domains) and sequence analysis using 3 regions of KHV genomic DNA (SphI-5, 9/5, and the thymidine kinase gene). In this study, samples from 6 carp showing symptoms of KHV infection in 2008 were examined for the presence of KHV by using PCR and cell culture isolation methods. KHV was detected in 2 (Pyeongtaek and Buan) of the samples. Sequence analysis revealed that the genotype of the KHV PT-08 isolate was Asia genotype variant 1 (A1), and the genotype of the KHV BA-08 isolate was European genotype variant 4 (E4). In addition, PCR patterns and sequence analysis based on the alleles of 3 domains of an alternate KHV classification system confirmed that the genotype of the KHV PT-08 isolate was CyHV3-J, and the genotype of the KHV BA-08 isolate was CyHV3-third genotype. To our knowledge, this is the first study to demonstrate the presence of 2 genotypes of KHV (genotype A1/CyHV3-J; genotype E4/CyHV3-third genotype) in South Korea.
Aquaculture | 2001
Ki Hong Kim; Yoon Jung Hwang; Se Ryun Kwon
Abstract To elucidate whether daily water temperature changes influence chemiluminescent response, plasma glucose level and mortality of cultured rockfish ( Sebastes schlegeli ), three experimental temperature groups were established. The fish in group I were maintained constantly at 27°C, and those in groups II and III were exposed to daily elevation of water temperature from 27°C to 28°C and 20°C to 22°C for 5 h, respectively. After 1 week of being maintained in each experimental tank, the fish in groups II and III showed significantly lower CL peak values ( P P
Korean Journal of Fisheries and Aquatic Sciences | 2014
Han-Gill Seo; Jung Soo Seo; Min-Kyung Ryu; Eun Hye Lee; Se Ryun Kwon; Jong Soon Kang; Yun-San No; Hye-Sung Choi; Sung Hee Jung; Hyun-Ja Han
We investigated a nematode infection in the epithelial tissue of rockfish Sebastes schlegeli cultured in Cheonsu Bay, western Korea, from May 2013 to April 2014. Nematodes infected the epithelial tissues of various external organs, including the fins, operculum, nares, mouth, and head. Over a 1-year period, the overall nematode infection rate in rockfish was 55% (n
Fish & Shellfish Immunology | 2006
Se Ryun Kwon; Yoon Kwon Nam; Sung Koo Kim; Ki Hong Kim
Diseases of Aquatic Organisms | 2004
Sung Mi Kim; Jae Bum Cho; Eun Hye Lee; Se Ryun Kwon; Sung Koo Kim; Yoon Kwon Nam; Ki Hong Kim
Aquaculture | 2007
Se Ryun Kwon; Eun Hye Lee; Yoon Kwon Nam; Sung Koo Kim; Ki Hong Kim
Aquaculture | 2005
Se Ryun Kwon; Yoon Kwon Nam; Sung Koo Kim; Dong Soo Kim; Ki Hong Kim
Diseases of Aquatic Organisms | 2002
Se Ryun Kwon; Chun Soo Kim; Joon Ki Chung; Hyung Ho Lee; Ki Hong Kim
Diseases of Aquatic Organisms | 2001
Ki Hong Kim; Eun Hae Lee; Se Ryun Kwon; Jae Bum Cho