Sebastian Schmidt

Cultivation of Hematopoietic Stem and Progenitor Cells: Biochemical Engineering Aspects

The ex vivo expansion of hematopoietic cells is one of the most challenging fields in cell culture. This is a rapidly growing area of tissue engineering with many potential applications in bone marrow transplantation, transfusion medicine or gene therapy. Over the last few years much progress has been made in understanding hematopoietic differentiation, discovery of cytokines, isolation and identification of cellular subtypes and in the development of a variety of bioreactor concepts. All this has led to a number of (preliminary) clinical trials that gave a hint of the benefits that can be obtained from the use of expanded hematopoietic cells in therapy. Moreover, as we understand the complexity and the regulation of hematopoiesis, it becomes obvious that highly sophisticated cultivation techniques and bioreactor concepts are needed: a new challenge for bioprocess engineering in cell culture.

Tools and applications of biochemical engineering science

F. Tholey, E. Heinzle: Methods for Biocatalyst Screening.- E. Stark, B. Hitzmann, K. Schugerl, T. Scheper: In-Situ-Fluorescence-Probes: A Useful Tool for Non-Invasive Bioprocess Monitoring.- C. Wittmann: Metabolic Flux Analysis Using Mass Spectrometry.- S. Nath: The Molecular Mechanism of ATP Synthesis by F1F0-ATP Synthase: A Scrutiny of the Major Possibilities.- I. Jasmund, A. Bader: Bioreactor Developments for Tissue Engineering Applications by teh Example of the Bioartificial Liver.- t. Noll, N. Jelinek, S. Schmidt, M. Biselli, C. Wandrey: Cultivation of Hematopoietic Stem and Progenitor Cells: Biochemical Engineering Aspects.- L.R. Castilho, R.A. Medronho: Cell Retention Devices for Suspended-Cell Perfusion Cultures.- N. Chang, S.J. Han, S.C. Inm, M.-R. Han, J. Lee: Fed-Batch Cultures of Escherichia coli Cells with Oxygen-Dependent nar Promoter Systems.- L. Maranga, P.E. Cruz, J.G. Aunins, M.J.T. Carrondo: Production of Core and Virus-Like Particles With Baculovirus Infected Insect Cells.- I. Wagner-Dobler, W. Beil, S. Lang, M. Meiners, H. Laatsch: Integrated Approach to Explore the Potential of Marine Microorganisms for the Production of Bioactive Metabolites.- A.-P. Zeng, H. Biebl: Bulk-Chemicals from Biotechnology: The Case of 1,3-Propanediol Production and the New Trends.