Seiji Ishikawa
Eisai
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Publication
Featured researches published by Seiji Ishikawa.
Analytical Biochemistry | 1986
Hiromitsu Yokohama; Issei Ohtsuka; Hiroyuki Shiojiri; Kouich Katayama; Seiji Ishikawa
Abstract A high-performance liquid chromatographic method has been developed for the measurement of cholesterol 7α-hydroxylase activity in liver microsomes. 7α-Hydroxycholesterol generated from endogenous choleserol was derivatized with anthroyl 1-carbonitrile, chromatographed on a reversephase column, and detected fluorometrically. The detection limit of 7α-hydroxycholesterol was 1 ng/tube. The cholesterol 7α-hydroxylase activity in rat liver microsomes was assayed by this method, and the effects of some detergents and of the addition of exogenous cholesterol together with detergents on the enzyme activity were investigated. The endogenous 7α- and 7β-hydroxy-cholesterol could be also measured by this method.
Journal of Pharmaceutical Sciences | 1990
Toshiro Tsuda; Mikio Uchiyama; Takashi Sato; Hiroshi Yoshino; Yutaka Tsuchiya; Seiji Ishikawa; Masahiko Ohmae; Sumio Watanabe; Yasuo Miyake
Bunseki Kagaku | 1984
Kouichi Abe; Kenichi Kusube; Keeko Mukasa; Yoshinobu Ishiguro; Tadashi Sato; Seiji Ishikawa; Haruhiko Hoshida
Chemical & Pharmaceutical Bulletin | 1983
Kenichi Kusube; Kouichi Abe; Osamu Hiroshima; Yoshinobu Ishiguro; Seiji Ishikawa; Haruhiko Hoshida
Journal of Pharmaceutical Sciences | 1990
Toshiro Tsuda; Mikio Uchiyama; Takashi Sato; Hiroshi Yoshino; Yutaka Tsuchiya; Seiji Ishikawa; Masahiko Ohmae; Sumio Watanabe; Yasuo Miyake
Chemical & Pharmaceutical Bulletin | 1983
Osamu Hiroshima; Satoru Ikenoya; Toshihiko Naitoh; Kenichi Kusube; Masahiko Ohmae; Kiyoshi Kawabe; Seiji Ishikawa; Haruhiko Hoshida; Tatsuo Kurahashi
The Journal of vitaminology | 1966
Seiji Ishikawa; Goichiro Katsui
The Journal of vitaminology | 1966
Goichiro Katsui; Seiji Ishikawa; Michiko Shimizu
Journal of Pharmaceutical Sciences | 1989
Toshiro Tsuda; Mikio Uchiyama; Takashi Sato; Hiroshi Yoshino; Yutaka Tsuchiya; Seiji Ishikawa; Masahiko Ohmae; Sumio Watanabe; Yasuo Miyake
The Journal of vitaminology | 1966
Seiji Ishikawa; Goichiro Katsui
