Seiji Nakao

The rate of autoxidation of oxymyoglobin in the presence of detergents

Abstract The rate of autoxidation of oxymoglobin was studied spectrophotometrically in the presence of detergents such as sodium laurate and 1-laurylpyridinium chloride. The rate was extremely accelerated near the critical micelle concentration of the detergents. It was only slightly dependent on the pH in the range from 8.5 to 10.5, but significantly dependent on ionic strength. After the fast denaturation of globin, caused by cluster formation around hydrophobic amino-acid residues of globin near the critical micelle concentration, autoxidation might occur with retention of a prosthetic group inside a heme pocket. It is proposed that the formation of clusters might shift both amino-acid residues and the prosthetic group from their normal positions inside the heme pocket, resulting in an increase of the rate of autoxidation.

Development of Solid Manure Injector

A solid manure injection system is a benifical method which reduces undesirable order and pollution problems from runoff. This system design consists of two basic units which are furrow opener and auger conveyer of manure.The manure tested first on the auger unit was bark of broadleaved tree. This material did not work into auger unit easily by stopping at the feed inlet port of auger unit. To solve this problem the tests were trid with lieqid (moisture content 78%) and dry (moisture content 13%) conditions of bark manure.The test results were as follows:(1) The bark manure on dry condition could not allow the revolution speed of auger shaft to work when the area of feed inlet port of auger unit was relatively small and the revolution speed of auger shaft was relatively high.(2) The injection volume of manure on liqid condition was much as compared with dry condtion on the same revolution speed and angle of auger shaft, but poor in the ingredient of mamure, because of water content of 78%.(3) The required torque of auger shaft on liqid condtion of manure was large with large revolution speed. The manure of liqid condition was dropped down by itself on 60° in the angle of auger shaft. Therefore, it was hard to control the volume of manure by the revolution of auger shaft.(4) The injection volume per required torque of auger shaft on liquied condition of manure, was much as compared with dry condition when the revolution speed and the angle of auger shaft were relatively small, and was less when they were large, The efficiency of injector was high on liqid condition when the area of feed inlet port was relatively small.(5) The solid manure injector that was attached the auger conveyer to furrow opener of shovel with the angle of 45° from above results, was tried to test on field. From the results, it could easily cotrol the injection volume of manure by changing the revolution speed of auger shaft.In realization, 2 or 3 drills of this device will be located at the front end of a box type manure spreader, in which the travel drection of the web will be reversed to bring the manure to the front. The device should inject the manure at a spacing which corresponds to row crop spacing and if it could use at the same time with seeding divice, a considerable reduction of production cost would be realized.