Seishiro Watanabe

Morphologic studies of the liver cell dysplasia.

Light and electron microscopic changes in human liver cells which were considered to be precancerous lesions, were studied. In our micrometrical examination, dysplastic liver cells were classified into two types: large and small dysplastic cell. Each type had nuclear pleomorphism and multinucleation; however, the nucleocytoplasmic ratio of the large dysplastic cell remained normal. Electron microscopically, the large dysplastic cell had some features of regenerative cells. The nucleocytoplasmic ratio of the small dysplastic cell was between that of normal hepatocytes and liver cancer cells. The difference in the incidence of the small and large dysplastic cells in normal livers and cirrhotic livers having hepatocellular carcinoma was statistically significant. In addition, the small dysplastic cell had more of a tendency to produce a small round focus. It was morphologically suggested that the more important candidate for the precancerous cell in the liver was the small dysplastic cell.

TYPE IV GLYCOGENOSIS - A STUDY OF TWO CASES

Liver biopsy materials of two siblings with type IV glycogenosis were studied by light and electron microscopy. Biochemical analysis was added using autopsy material in one of the two cases. Two kinds of polysaccharides were noted not only in the cardiac muscle, skeletal muscles, smooth muscles and reticuloendothelial cells, but also in the neutrophils and platelets. One was glycogen and the other was similar to amylopectin. Ultrastructurally, a large amount of fibrils, 60 A in width, glycogen rosettes and glycogen granules were detected in those cells. Branching glycosyl‐transferase deficiency was biochemically confirmed in one case examined.

Promotive effect of phenobarbital through hepatocarcinogenesis by 2-fluorenylacetamide in rats

肝細胞癌の発生機転(initiation)にHBウイルスやAlfatoxin B1などの化学物質の関与が論じられているが,未だ癌発生のもう1つの機転であるpromotorについては不明である.そこで,promotor解析のスクリーニング法を確立する目的でPerainoらの実験を追試し,彼らが38週という長期間の観察を要したことも,前癌性病変の出現に焦点をあてた場合,13週という短時間でもフェノバルビタールの発癌促進効果を明らかにしえた.さらに成熟ラットと離乳期ラットではフェノバルビタールの促進効果に違いがみられ,年齢による薬物代謝の相違点が明らかにされた.