Seiya Chiba
Hokkaido University
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Featured researches published by Seiya Chiba.
FEBS Letters | 1994
Hirokazu Kawagishi; Hironobu Mori; Akinori Uno; Atsuo Kimura; Seiya Chiba
A lectin was isolated from the mushroom Hericium erinaceum. This lectin is composed of two different subunits of 15 and 16 kDa and the molecular mass of the intact lectin was estimated to be 54 kDa by gel filtration. It exhibits specificity towards sialic acids, especially N‐glycolylneuraminic acid.
Phytochemistry | 1997
Hirokazu Kawagishi; Shin-Ichiro Mitsunaga; Masamichi Yamawaki; Mitoko Ido; Atsushi Shimada; Tetsuya Kinoshita; Takeomi Murata; Taichi Usui; Atsuo Kimura; Seiya Chiba
A lectin (GLL-M) was isolated from mycelia of Ganoderma lucidum using affinity chromatography on BSM-Toyopearl. GLL-M is a monomer in its native form with a M(r) of 18,000. Another lectin was also purified from fruiting bodies of the same fungus. The two lectins were partially compared with each other.
Journal of Biotechnology | 1997
Akira Nakamura; Ikuko Nishimura; Akihito Yokoyama; Dong-Gun Lee; Makoto Hidaka; Haruhiko Masaki; Atsuo Kimura; Seiya Chiba; Takeshi Uozumi
We have cloned an extracellular alpha-glucosidase gene from Aspergillus niger with oligonucleotide probes synthesized on the basis of the determined peptide sequences. The nucleotide sequence revealed an open reading frame of 985 amino acids split with three introns, and the deduced amino acid sequence was nearly identical to that of the alpha-glucosidase previously determined. The cloned gene was introduced into Aspergillus nidulans, and its expression in the transformants was shown to be regulated by the carbon sources in the medium, suggesting that a common regulatory expression system is shared by these two species as is the case of other starch-degrading enzymes of Aspergillus species.
Carbohydrate Research | 1994
Tsutomu Takayanagi; Atsuo Kimura; Seiya Chiba; Katsumi Ajisaka
Abstract Seven oligosaccharides were isolated from α- d -glucosidase (EC 3.2.1.20) from Aspergillus niger, and the structures of these oligosaccharides were studied by 1H NMR spectroscopy. After treatment of the α- d -glucosidase with N-glycosidase F, seven major oligosaccharide peaks were detected by Dionex anion-exchange HPLC. The structures corresponding to the three peaks OS-1, OS-2, and OS-4 were determined to be Man8GlcNAc2, and GlcMan9GlcNAc2, respectively, from 1H NMR spectra of the isolated fractions. Each of the four oligosaccharides OS-5, OS-6, OS-7-1, and OS-7-2 contained an α- d -galactofuranosyl residues (Galf) linked to Man (A) via an α-(1 → 2)-linkage. OS-7 was found to consist of two oligosaccharides. The structures of these four oligosaccharides were determined to be GalfMan5GlcNAc2, GalfMan6GlcNAc2, GalfMan7-GlcNAc2, and GalfMan8GlcNAc2 by 1H NMR spectroscopy and compositional analysis. The Galf structure of GalfMan5GlcNAc2 was found to be identical to that of an oligosaccharide previously isolated from the α- d -galactosidase of the same strain1. The structure of OS-3 remains undetermined.
Bioscience, Biotechnology, and Biochemistry | 1997
Seiya Chiba
Bioscience, Biotechnology, and Biochemistry | 2001
Mamoru Nishimoto; Masaki Kubota; Masahisa Tsuji; Haruhide Mori; Atsuo Kimura; Hirokazu Matsui; Seiya Chiba
Bioscience, Biotechnology, and Biochemistry | 1996
Shuichi Onodera; Toyotaka Murakami; Hiroyuki Ito; Haruhide Mori; Hirokazu Matsui; Mamoru Honma; Seiya Chiba; Norio Shiomi
Bioscience, Biotechnology, and Biochemistry | 1995
Shunsuke Iwanami; Hirokazu Matsui; Atsuo Kimura; Hiroyuki Ito; Haruhide Mori; Mamoru Honma; Seiya Chiba
Bioscience, Biotechnology, and Biochemistry | 1997
Atsuo Kimura; Masuhiro Takata; Yukiharu Fukushi; Haruhide Mori; Hirokazu Matsui; Seiya Chiba
Bioscience, Biotechnology, and Biochemistry | 2000
Ryu Su-Jin; Doman Kim; Hwa-Ja Ryu; Seiya Chiba; Atsuo Kimura; Donal F. Day