Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where Seiya Chiba is active.

Publication


Featured researches published by Seiya Chiba.


FEBS Letters | 1994

A sialic acid-binding lectin from the mushroom Hericium erinaceum

Hirokazu Kawagishi; Hironobu Mori; Akinori Uno; Atsuo Kimura; Seiya Chiba

A lectin was isolated from the mushroom Hericium erinaceum. This lectin is composed of two different subunits of 15 and 16 kDa and the molecular mass of the intact lectin was estimated to be 54 kDa by gel filtration. It exhibits specificity towards sialic acids, especially N‐glycolylneuraminic acid.


Phytochemistry | 1997

A lectin from mycelia of the fungus Ganoderma lucidum

Hirokazu Kawagishi; Shin-Ichiro Mitsunaga; Masamichi Yamawaki; Mitoko Ido; Atsushi Shimada; Tetsuya Kinoshita; Takeomi Murata; Taichi Usui; Atsuo Kimura; Seiya Chiba

A lectin (GLL-M) was isolated from mycelia of Ganoderma lucidum using affinity chromatography on BSM-Toyopearl. GLL-M is a monomer in its native form with a M(r) of 18,000. Another lectin was also purified from fruiting bodies of the same fungus. The two lectins were partially compared with each other.


Journal of Biotechnology | 1997

Cloning and sequencing of an α-glucosidase gene from Aspergillus niger and its expression in A. nidulans

Akira Nakamura; Ikuko Nishimura; Akihito Yokoyama; Dong-Gun Lee; Makoto Hidaka; Haruhiko Masaki; Atsuo Kimura; Seiya Chiba; Takeshi Uozumi

We have cloned an extracellular alpha-glucosidase gene from Aspergillus niger with oligonucleotide probes synthesized on the basis of the determined peptide sequences. The nucleotide sequence revealed an open reading frame of 985 amino acids split with three introns, and the deduced amino acid sequence was nearly identical to that of the alpha-glucosidase previously determined. The cloned gene was introduced into Aspergillus nidulans, and its expression in the transformants was shown to be regulated by the carbon sources in the medium, suggesting that a common regulatory expression system is shared by these two species as is the case of other starch-degrading enzymes of Aspergillus species.


Carbohydrate Research | 1994

Novel structures of N-linked high-mannose type oligosaccharides containing α-d-galactofuranosyl linkages in Aspergillus niger α-d-glucosidase

Tsutomu Takayanagi; Atsuo Kimura; Seiya Chiba; Katsumi Ajisaka

Abstract Seven oligosaccharides were isolated from α- d -glucosidase (EC 3.2.1.20) from Aspergillus niger, and the structures of these oligosaccharides were studied by 1H NMR spectroscopy. After treatment of the α- d -glucosidase with N-glycosidase F, seven major oligosaccharide peaks were detected by Dionex anion-exchange HPLC. The structures corresponding to the three peaks OS-1, OS-2, and OS-4 were determined to be Man8GlcNAc2, and GlcMan9GlcNAc2, respectively, from 1H NMR spectra of the isolated fractions. Each of the four oligosaccharides OS-5, OS-6, OS-7-1, and OS-7-2 contained an α- d -galactofuranosyl residues (Galf) linked to Man (A) via an α-(1 → 2)-linkage. OS-7 was found to consist of two oligosaccharides. The structures of these four oligosaccharides were determined to be GalfMan5GlcNAc2, GalfMan6GlcNAc2, GalfMan7-GlcNAc2, and GalfMan8GlcNAc2 by 1H NMR spectroscopy and compositional analysis. The Galf structure of GalfMan5GlcNAc2 was found to be identical to that of an oligosaccharide previously isolated from the α- d -galactosidase of the same strain1. The structure of OS-3 remains undetermined.


Bioscience, Biotechnology, and Biochemistry | 1997

Molecular Mechanism in α-Glucosidase and Glucoamylase

Seiya Chiba


Bioscience, Biotechnology, and Biochemistry | 2001

Purification and Substrate Specificity of Honeybee, Apis mellifera L., α-Glucosidase III

Mamoru Nishimoto; Masaki Kubota; Masahisa Tsuji; Haruhide Mori; Atsuo Kimura; Hirokazu Matsui; Seiya Chiba


Bioscience, Biotechnology, and Biochemistry | 1996

Molecular Cloning and Nucleotide Sequences of cDNA and Gene Encoding endo-Inulinase from Penicillium purpurogenum

Shuichi Onodera; Toyotaka Murakami; Hiroyuki Ito; Haruhide Mori; Hirokazu Matsui; Mamoru Honma; Seiya Chiba; Norio Shiomi


Bioscience, Biotechnology, and Biochemistry | 1995

Chemical Modification and Amino Acid Sequence of Active Site in Sugar Beet α-Glucosidase

Shunsuke Iwanami; Hirokazu Matsui; Atsuo Kimura; Hiroyuki Ito; Haruhide Mori; Mamoru Honma; Seiya Chiba


Bioscience, Biotechnology, and Biochemistry | 1997

A Catalytic Amino Acid and Primary Structure of Active Site in Aspergillus niger α-Glucosidase

Atsuo Kimura; Masuhiro Takata; Yukiharu Fukushi; Haruhide Mori; Hirokazu Matsui; Seiya Chiba


Bioscience, Biotechnology, and Biochemistry | 2000

Purification and partial characterization of a novel glucanhydrolase from Lipomyces starkeyi KSM 22 and its use for inhibition of insoluble glucan formation.

Ryu Su-Jin; Doman Kim; Hwa-Ja Ryu; Seiya Chiba; Atsuo Kimura; Donal F. Day

Collaboration


Dive into the Seiya Chiba's collaboration.

Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Top Co-Authors

Avatar
Researchain Logo
Decentralizing Knowledge