Seiya Yonemori

Measurement of OH, O, and NO densities and their correlations with mouse melanoma cell death rate treated by a nanosecond pulsed streamer discharge

Mouse melanoma cells in a culture medium are treated using a nanosecond pulsed streamer discharge plasma and the correlations between the rate of cell death and the densities of reactive species (OH, O, and NO) in the plasma are measured. The plasma is irradiated onto the culture medium surface with a vertical gas flow of an O2/N2 mixture from a glass tube at various gas flow rates and O2 concentrations. The densities of the reactive species are measured very close to the culture medium surface, where the reactive species interact with the culture medium, using laser-induced fluorescence. In the case of the N2 discharge (O2 = 0%), an increase in gas flow rate decreases OH density because it lowers the water vapor concentration by diluting the vapor, which is required for OH production. The increase in gas flow rate also leads to a decreased cell death rate. In the case of the O2/N2 discharge, on the other hand, an increase in O2 concentration at a fixed flow rate does not affect the rate of cell death, although it considerably changes the O and NO densities. These findings indicate that some reactive species derived from water vapor such as OH are responsible for the melanoma cell death, whereas those from O2, such as O and NO, are less likely responsible. They also indicate the importance of water evaporation from the culture medium surface in cell treatment.

Effect of discharge polarity on the propagation of atmospheric-pressure helium plasma jets and the densities of OH, NO, and O radicals

The atmospheric-pressure helium plasma jet is an emerging technology for plasma biomedical applications. In this paper, the authors focus on the effect of discharge polarity on propagation of the discharge and the densities of OH, NO, and O radicals. The plasma jet is applied to a glass surface placed on a grounded metal plate. Positive or negative voltage pulses with 25 μs duration, 8 kV amplitude, and 10 kpps repetition rate are used for the plasma jet. The plasma propagation is measured using a short-gated ICCD camera. The light emission intensity of the discharge generated at the rising phase of the voltage pulse is approximately equivalent for both polarities, while that generated during the falling phase is much higher for the negative discharge than the positive one. The shape of the discharge changes with the discharge polarity. The OH, NO, and O densities in the plasma jet are also measured for both polarities. It is found that the OH density is almost the same regardless the discharge polarity. Conversely, the negative discharge produces more O atoms and the positive discharge produces more NO molecules. These results indicate that the polarity of the discharge affects the densities of some reactive species produced in the plasma jet.

Inactivation of Bacillus atrophaeus by OH radicals

The inactivation of Bacillus atrophaeus by OH radicals is measured. This study aims to evaluate the bactericidal effects of OH radicals produced by atmospheric-pressure nonthermal plasma widely used for plasma medicine; however, in this study, OH radicals are produced by vacuum ultraviolet (VUV) photolysis of water vapor instead of plasma to allow the production of OH radicals with almost no other reactive species. A 172 nm VUV light from a Xe2 excimer lamp irradiates a He–H2O mixture flowing in a quartz tube to photodissociate H2O to produce OH, H, O, HO2, H2O2, and O3. The produced reactive oxygen species (ROS) flow out of the quartz tube nozzle to the bacteria on an agar plate and cause inactivation. The inactivation by OH radicals among the six ROS is observed by properly setting the experimental conditions with the help of simulations calculating the ROS densities. A 30 s treatment with approximately 0.1 ppm OH radicals causes visible inactivation.

Measurement of OH radical in the effluent of an atmospheric-pressure helium plasma jet

Atmospheric-pressure helium plasma jet is getting much attention especially in plasma medical application or dental application. It is thought that active species play important role in the plasma processes and they are generated from the interaction between the plasma and molecules included in the ambient air and the discharge gas. OH radical is one of the most important active species. However its production mechanism is not yet elucidated completely. In this study, OH density distribution in the vicinity of dry and wet surface in the effluent of an atmospheric pressure helium plasma jet was measured by using laser induced fluorescence (LIF). OH density and its distribution varied depending on some parameters; helium flow rate, gap between the surface and the end of capillary tube and humidity of the objective surface. The maximum OH density was approximately 1.0 ppm in the case of the plasma jet extending toward wet surface when high voltage 10 kV, 8 kHz was applied. The result suggests that OH radical is produced by the dissociation of H2O nearby the surface that evaporates from the surface or adheres on the surface.