Selene Dall'Acqua Coutinho

Survey of bovine mycotic mastitis in dairy herds in the State of São Paulo, Brazil

The purpose of this study was to isolate fungi from the quarter milk of cow udders from several dairy herds and to identify the different genera and species involved in mastitis. A total of 2078 milk samples from normal, clinical and subclinical mastitis quarters from 22 dairy herds of 16 districts in the State of São Paulo, Brazil was utilized in this survey. Two hundred and fifty one (12.07%) fungi were isolated from the samples. Two hundred and eight of these (82.86%) were yeasts and 30 (11.95%) were moulds. The fungi were isolated in pure culture (24.77%) or in cultures mixed with bacteria (72.22%). The yeasts isolated were:Cryptococcus spp. (71 strains),Rhodotorula spp. (40),Candida spp. (68),Trichosporon cutaneum (21),Aureobasidium pullulans (7), andPichia ohmeri (1). Moulds classified in following genera were also isolated:Aspergillus (3),Penicillium (3),Alternaria (3),Phoma (3),Epicoccum (2), andGeotrichum (16).

Protein profiles of Malassezia pachydermatis isolated from dogs.

The protein fractions of 30 Malassezia pachydermatis isolates from dogs (15 with otitis and 15 with dermatitis) were investigated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The electrophoresis runs resulted in bands ranging from 15.0–145.7 kDa in the ear canal secretion isolates. The values for the skin isolates varied between 16.0–141.1 kDa. The presence of protein bands occurred most frequently within the 30–40 kDa bands (66.7%), 40–50 kDa (70.0%), 50–60 kDa (73.3%) and 80–90 kDa (83.3%). No statistical differences were determined, considering the behavior of M. pachydermatis isolates from ear and skin in each band of molecular weight values, nor in the number of bands obtained.