Selma Arzu Vardar

Psychological symptoms and sleep quality in young subjects with different circadian preferences

The aim of the study was to investigate the psychological features, sleep quality and daytime sleepiness of different chronotypes in healthy young participants. Seventy-nine female and 63 male medical students aged between 17 and 23 years (mean age: 19.8 ± 1.3 years) participated voluntarily in this study. They completed the Morningness-Eveningness Questionnaire, self-reported symptom inventory SCL-90-R, the Pittsburgh Sleep Quality Index and the Epworth Sleepiness Scale. Psychological symptoms and sleep features of morning types, evening types and intermediate types were compared with each other. Out of 141 subjects, 30 were evening types (21%), 34 were morning types (24%) and 77 were intermediate types (55%). Positive-symptom total scores of SCL-90-R were higher in the evening types than the morning and intermediate types. There were significant differences in the psychological symptoms of anxiety, obsessive-compulsiveness, hostility and phobia among these groups (p < 0.05). Total sleep quality was poorer and daytime sleepiness was significantly higher in the evening types than the morning and intermediate types (p < 0.05). Our findings suggest that evening chronotypes suffer from more anxiety, obsessive-compulsiveness, hostility and phobia symptoms, sleep problems and daytime sleepiness than the other chronotypes.

The effect of high protein diet and exercise on irisin, eNOS, and iNOS expressions in kidney.

Abstract Long-term effects of high protein diets (HPDs) on kidneys are still not sufficiently studied. Irisin which increases oxygen consumption and thermogenesis in white fat cells was shown in skeletal muscles and many tissues. Nitric oxide synthases (NOS) are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. We aimed to investigate the effects of HPD, irisin and NO expression in kidney and relation of them with exercise and among themselves. Animals were grouped as control, exercise, HPD and exercise combined with HPD (exercise-HPD). Rats were kept on a HPD for 5 weeks and an exercise program was given them as 5 exercise and 2 rest days per week exercising on a treadmill with increasing speed and angle. In our study, while HPD group had similar total antioxidant capacity (TAC) levels with control group, exercise and exercise-HPD groups had lower levels (p < 0.05). Kidneys of exercising rats had no change in irisin or eNOS expression but their iNOS expression had increased (p < 0.001). HPD–E group has not been observed to cause kidney damage and not have a significant effect on rat kidney irisin, eNOS, or iNOS expression. Localization of irisin, eNOS, and iNOS staining in kidney is highly selective and quite clear in this study. Effects of exercise and HPD on kidney should be evaluated with different exercise protocols and contents of the diet. İrisin, eNOS, and iNOS staining localizations should be supported with various research studies.

Effects of sleep deprivation on anaerobic exercise-induced changes in auditory brainstem evoked potentials

Background  The present study was designed to assess how anaerobic exercise affects auditory brainstem response (ABR) parameters, and whether one night of sleep deprivation could alter these possible exercise‐induced changes in ABRs.

High fructose diet suppresses exercise-induced increase in AQP7 expression in the in vivo rat heart.

Objective: Cardiac uptake of fructose is thought to be mediated by glucose transporter 5 (GLUT5), whereas the uptake of glycerol is facilitated by aquaporin 7 (AQP7). We aimed to investigate the effect of a high-fructose diet (HFD) on GLUT5 and AQP7 levels in the rat heart subjected to exercise. Methods: Male Sprague–Dawley rats were allocated to control (C; n=11), exercise (E; n=10), HFD (n=12), and HFD plus exercise (HFD-E; n=12) groups. HFD was started 28 days before euthanasia. From day 24 to 27, rats were subjected to moderate exercise, followed by vigorous exercise on day 28 (groups E and HFD-E). Cardiac GLUT5 and AQP7 mRNA levels were determined using RT-PCR. The protein contents of GLUT5 and AQP7 were immunohistochemically assessed. Paired-t, ANOVA with Bonferroni, Kruskal–Wallis, and Bonferroni-corrected Mann–Whitney U tests were used for statistical analysis. Results: GLUT5 mRNA expression and protein content did not differ between the groups. AQP7 mRNA levels significantly increased (4.8-fold) in group E compared with in group C (p<0.001). Compared with group C, no significant change was observed in AQP7 mRNA levels in groups HFD and HFD-E. The AQP7 staining score in group E was significantly higher than that in groups C (p<0.001), E (p<0.001), and HFD-E (p<0.001). Conclusion: Our study indicates that exercise enhances cardiac AQP7 mRNA expression and protein content. However, HFD prevents the exercise-induced increase in cardiac AQP7 expression. This inhibitory effect may be related to the competition between fructose and glycerol as energy substrates in the rat heart subjected to 5 days of physical exercise. (Anatol J Cardiol 2016; 16: 916-22)

High-intensity interval training acutely alters plasma adipokine levels in young overweight/obese women

Abstract The aim of this study was to investigate the plasma adipokine responses to high-intensity interval training (HIT) in overweight/obese women. Twelve women (age 21.7 ± 3.8 years) completed a 19 days of HIT comprising six session of 4–6 repeats of a Wingate test (0.065 kg load/kg). Plasma adipokine levels were measured before exercise, and at 5 and 90 min after exercise on the first and the last training days. Adiponectin was higher at 5 min than 90 min post-exercise (11.7 ± 7.3 and 10.5 ± 5.8 ng/ml; p = .01) in the first exercise day. Leptin decreased 5 min after exercise (23.6 ± 13.2 vs. baseline 27.8 ± 14.4 ng/ml; p < .01) and remained depressed following 90 min (p < .01). The changes in adiponectin and leptin concentrations were similar on the first and last exercise days. No consistent effect was found on resistin concentration. Future studies are required to disclose the functional consequences of these alterations in plasma adipokine levels.

The alteration of NTproCNP plasma levels following anaerobic exercise in physically active young men

Objective: Amino-terminal propeptide of C-type natriuretic peptide (NTproCNP) is a synthesis product of C-type natriuretic peptide (CNP). In this study, plasma levels of NTproCNP were compared before and after exercise in healthy young subjects who are physically active (PA) or not physically active (NPA). Methods: The study was carried on PA group (n=10) who defined the exercise duration more than 2.5 hours per week for at least one year and NPA group (n=10) whose exercise duration was lower than 1.5 hours per week. The level of maximal oxygen consumption was determined. Wingate exercise test was applied on the following day. Plasma NTproCNP levels were measured before the exercise and at the 1st, 5th and 30th minute after the exercise. Results: Exercise duration of physically active group was reported as 11.3±5.0 hours per week. Basal NTproCNP levels of the groups were found to be comparable. NTproCNP levels in the 5th minute (0.93±0.23 pmol/L; p<0.05) and in the 30th minute (0.77±0.21 pmol/L p<0.05) after exercise were higher than the levels before exercise (0.64±0.29 pmol/L) in PA group. Additionally, the plasma levels of NTproCNP after 5th minute of exercise were higher in PA group (0.93±0.23 pmol/L) than NPA group (0.74±0.16 pmol/L, p<0.05). Conclusion: Being physically active may be a fact affecting the secretion of CNP, which plays a protective role in endothelium, following exercise.

Egzersiz bağımlılığı ve psikopatolojik özelliklerinin değerlendirilmesi / Exercise dependence and evaluations of psychopathological features

Exercise dependence and evaluations of psychopathological features Objective: Exercise dependence has been described as making excessive physical exercise leading to clinically significant negative consequences. Self report scales were developed for the diagnosis of exercise dependence based on DSM-IV criteria. For the diagnosis, exercise frequency, psychological factors (e.g. pathological commitment), tolerance and withdrawal symptoms should be taken into consideration. However, the diagnosis of exercise dependence has been neglected because its definition is unsatisfactory and exercise dependent subjects do not seek any help. Secondary exercise dependence is defined as compulsive exercise occurring with eating disorders. The aim of this study is to investigate the exercise dependence in subjects practicing sports regularly and to evaluate the psychopathological features and disordered eating behaviors in the exercise dependent subjects. Method: One hundred fifteen voluntary subjects who had exercised regularly at least three times a week throughout the previous year were evaluated by Exercise Dependence Scale-21 (EDS-21), Symptom Check List 90-Revised (SCL-90-R) form and Eating Attitude Test (EAT-40). The subjects were categorized into three groups as exercise dependent, non-dependent symptomatic and non-dependent asymptomatic according to EDS-21 results and the groups were compared with each other. Results: Exercise dependence was present in 14 (12%) of all subjects. Of the exercise dependent subjects, 85% met the physiological dependence criteria. Exercise dependent group did not statistically differ from the other groups in terms of psychopathological symptoms and eating behavior. Conclusions: Our results showed that eating behavior and psychopathological features in exercise dependent subjects were not different from those of the non- dependent symptomatic and asymptomatic subjects. Exercise dependent subjects in the present study were categorized as having primary exercise dependence because they had no psychological and eating disorders symptoms. Psychological features and eating behavior should be evaluated together for the diagnosis of primary exercise dependence in subjects who exercised excessively and differential diagnosis should be made from the eating disorders.

Melatonin administration acutely decreases the diffusing capacity of carbon monoxide in human lungs

Background: Most physiological measurements of the pulmonary diffusing capacity use carbon monoxide (CO) as a tracer gas. Similar to CO, melatonin binds the hemoglobin in the blood. Objective: The present study was designed to assess the effect of exogenous melatonin administration on pulmonary functions including diffusing capacity for carbon monoxide (DLCO) in healthy subjects. Methods: The study was performed in a randomized, double-blind, placebo-controlled manner. DLCO was measured in 22 healthy male volunteers (age 18–25 years) who were randomized to melatonin (n = 11) and placebo administration (n = 11). At baseline, DLCO, alveolar volume (VA) and other spirometric parameters such as forced expiratory volume in 1 s (FEV1), forced vital capacity (FVC), peak expiratory flow (PEF) and maximal voluntary ventilation (MVV) were measured. DLCO was then corrected for the hemoglobin concentration. Measurements were repeated in a double-blind fashion 60 min after the administration of melatonin (1 mg) or placebo. Results: DLCO was significantly decreased (39.31 ± 4.75 vs. 34.82 ± 6.18 ml/min/mm Hg) 60 min after the melatonin administration (p = 0.01), while FEV1, FVC, FEV1/FVC, PEF and MVV values did not demonstrate significant differences. Placebo administration did not result in significant alteration in any of these parameters. Conclusions: In healthy subjects, oral administration of melatonin acutely influences the DLCO without affecting other pulmonary function test results. We conclude that melatonin may have a reducing effect on the DLCO in the lungs.

The alteration of asymmetric dimetilarginine (ADMA) levels in cardiac and gastrocnemius muscles following radioactive iodine application in guinea pigs and the effect of L-carnitine on this alteration

Purpose: Tissue levels of asymmetric dimetilarginine (ADMA) and symmetric dimetilarginine (SDMA) were investigated in cardiac ventricle and gastrocnemius muscles of guinea pigs treated with radioactive iodine (RAI) alone or in combination with L-carnitine (LC). Material and methods: Group 1 received no treatment (control group). Group 2 received a total dose of 30 mCi−1kg−1 body weight iodine-131 alone. Group 3 received 200 mg−1kg−1 of LC for 10 days alone. Group 4 received 200 mg−1kg−1 of LC plus RAI therapy. Free thyroid hormones, ADMA and SDMA concentrations were measured. Results: Serum free thyroid hormone concentrations were found decreased in the RAI and LC-RAI groups after RAI application. A significant decrease in ADMA and SDMA concentration was observed in ventricle muscle following RAI application. The LC-RAI group had significantly decreased ADMA levels in ventricle muscle compared with those of the control group. Similarly, SDMA concentrations in ventricle and gastrocnemius muscles of the LC-RAI groups were significantly lower than those of the control groups. Conclusions: Our results indicated that RAI appears to exert an inhibitory effect on ADMA and SDMA levels of ventricular muscle. LC administration when given adjuvant to RAI therapy may cause a marked decrease in ADMA concentrations of both ventricular and gastrocnemius muscles.

Okçuluk Sporcularında Plazma Atriyal Natriüretik Peptid Düzeyinin Postüre Bağlı Değişimi

Objectives: The aim of this study was to investigate plasma atrial natriuretic peptid levels in sitting and supine positions and cardiac functions in young male archery athletes. Materials and Methods: Six archery athletes and 10 sedentary males volunteered to participate in the study. Venous blood samples were taken after 10 minutes sitting and after 10 minutes in supine position. Plasma ANP levels were measured by the ELISA method. Cardiac parameters were assessed by echocardiography. Results: Plasma ANP levels in sitting position were higher in archery athletes (72.12aplusmn;14.01 pg/ ml) than sedentaries (36.22aplusmn;14.51 pg/ml; palt;0.01). Similarly, plasma ANP levels in supine position (124.41aplusmn;14.66 pg/ml) were significantly higher in the athletes than sedentaries (36.95aplusmn;9.83 pg/ml). Plasma ANP levels were higher in supine position than those in sitting position in archery athletes (palt;0.01). No significant increase in plasma ANP levels were found in supine position than sitting position in sedentary group. Echocardiographic parameters were similar in athletes and sedentary subjects. Conclusion: It is concluded that regular archery exercises without prominent functional and morphological alterations in the heart may cause differences in plasma ANP levels. Marked posture-related alterations may occur in plasma ANP levels of trained archery athletes. Amac: Bu calismanin amaci, okculuk sporu yapan genc erkek sporcularin plazma atriyal natriuretik peptid (ANP) duzeylerinde oturur ve yatar durumdaki degisimi ve kardiyak fonksiyonlarini incelemektir. Gerecler ve Yontemler: Calismaya, gonullu 6 okculuk sporcusu ve 10 sedanter erkek katildi. Katilimcilardan 10 dka#39;lik oturma sonrasi ve 10 dka#39;lik yatar durumdan sonra venoz kan alindi. Plazma ANP duzeyleri ELISA yontemi ile olculdu. Kardiyak parametreler ekokardiyografi ile belirlendi. Bulgular: Okculuk sporcularinin oturur durumdaki plazma ANP duzeylerinin (72.12aplusmn;14.01 pg/ml) sedanter grubun oturur durumdaki ANP duzeylerinden (36.22aplusmn;14.51 pg/ml) yuksek oldugu bulundu (palt;0.01). Ayni sekilde okculuk sporcularinin yatar durumdaki ANP duzeyleri de (124.41aplusmn;14.66 pg/ml) sedanterlere gore (36.95aplusmn;9.83 pg/ml) anlamli yukseklik gosterdi (palt;0.01). Sporcu grubun plazma ANP duzeyleri oturur duruma gore yatar durumda artmis bulundu (palt;0.01). Sedanter grubun ANP duzeylerinde ise oturur duruma gore yatar durumda anlamli bir artis saptanmadi. Sporcu ve sedanterlerin ekokardiyografik parametreleri benzer bulundu. Sonuc: Calismamiza gore okculukla ilgili yapilan duzenli egzersizler, kalpte belirgin fonksiyonel ve morfolojik degisim olmaksizin, plazma ANP duzeylerinde farkliliklara neden olabilir. Okculuk sporu yapanlarin plazma ANP duzeyinde posturle iliskili belirgin farkliliklar olusabilir.