Serdar Savaş Gül

The diagnostic utility of scintigraphy in esophageal burn: a rat model

BACKGROUND Corrosive esophageal injury due to accidental ingestion is a serious clinical problem in children particularly in developing countries. The present study was conducted to evaluate the diagnostic utility of technetium-99m-pyrophosphate ((99m)Tc-PYP) scintigraphy in the early stage of esophageal burns by using different concentrations of sodium hydroxide (NaOH) in an experimental rat model. MATERIALS AND METHODS Twenty-eight male Sprague-Dawley rats, weighing 200-250 g, were used in the study. Esophageal burn model was created in 21 rats by gastrically infusion of various concentrations of NaOH. The rats were divided randomly into three groups: mild-burn group (n = 7) received 15% NaOH, moderate-burn group (n = 7) received 30% NaOH and severe-burn group (n = 7) received 45% NaOH. Seven rats were identified as control group and received normal saline. Three hours after burn injury, 1-mCi (99m)Tc-PYP was administered through tail vein. Two hours after (99m)Tc-PYP administration, static imaging with gamma camera was performed. Then, histopathologic assessment of esophageal samples was achieved properly. RESULTS All NaOH-applied groups (mild, moderate, and severe) showed a significant higher uptake ratio when compared to control group (P < 0.005). NaOH-applied groups displayed important histologic alterations such as mucosal disintegration, edema, inflammation, and stromal damage when compared to control group. Pearson correlation analysis revealed a significant correlation between the (99m)Tc-PYP uptake ratio and histologic score (P < 0.0005). CONCLUSIONS The scintigraphic imaging may provide advantages in the early stage of esophageal burns in some patients whom endoscopic procedure is contraindicated because of its high risk of complications such as bleeding and perforation.

Kemiğin dev hücreli tümörü taklit eden primer hiperparatiroidizme bağlı brown tümörü

Primer hiperparatiroidizm (PHP) parathormon seviyelerinin yuksekligi ile karakterize endokrin bir hastaliktir. Hiperparatiroidizm komplikasyonlarindan biri olan Brown tumoru, osteoklastik aktivite sonucu ortaya cikar ve iskelet sisteminde lezyonlar, patolojik kirik ve agriya sebep olabilir . Brown tumoru hem histopatolojik hem de radyolojik incelemelerde primer kemik tumoru, anevrizmal kemik kisti veya kemigin dev hucreli tumoru ile karisabilir. Bu sunumda metastatik kemik tumorunden suphelenilen ancak primer hiperparatroidi tanisi alan 50 yasinda bayan hastanin sunumu yapilmaktadir. Brown tumorleri benign karakterli lezyonlardir ama malign kemik hastaliklarini taklit edebilirler. Hastaligin ayirici tanisinda klinik, histopatolojik ve radyolojik bulgularin birlikte degerlendirilmelidir.

Caecum location in laboratory rats and mice: an anatomical and radiological study

Intraperitoneal (i.p.) injection is the most frequently used method for implementing parenteral therapies in rats and mice. Whether the caecum is located in the right caudal quadrant or left caudal quadrant in the abdominal cavity is not clear. For that reason, we have developed a method for identifying the location of the caecum in rats and mice and thus revealed the most reliable location for i.p. injection in these animals. Two hundred Wistar albino rats and 100 BALB/c mice were used. The location of the caecum was determined by revealing the intra-abdominal organs immediately following euthanasia, photographing the organs, and archiving the images. Both digital photographic images and computed tomographic (CT) sections were analysed in terms of caecum morphology and location. In both rats and mice, the caecum was most commonly located on the animal’s left side. It was less frequently located on the right side or in the centre. The caecum was typically comma-shaped, but it was round or S-shaped in some animals. The direction of rotation of the caecum from the basis to the apex was mostly counterclockwise. Additionally, the apex showed a tendency to be evenly centred. This study demonstrated that the caecum was mostly located on the animal’s left side; and for that reason, the most suitable location for i.p. injection in these animals was understood to be the right caudal quadrant. Furthermore, when we compared the CT images and autopsy findings, the caecum did not change location in the abdominal cavity postmortem.