Sergey V. Tarlachkov

Draft Genome Sequence of “Rathayibacter tanaceti” Strain VKM Ac-2596 Isolated from Tanacetum vulgare Infested by a Foliar Nematode

ABSTRACT The draft genome of “Rathayibacter tanaceti” VKM Ac-2596 is 3.17 Mb in size with an average G+C content of 70.7% and comprises at least two nonidentical copies of ribosomal small subunit (SSU-rRNA) genes. The semiconductor sequencing platform Ion Torrent was used.

Genomics and Biochemistry of Metabolic Pathways for the C 1 Compounds Utilization in Colorless Sulfur Bacterium Beggiatoa leptomitoformis D-402

The metabolic pathways of one-carbon compounds utilized by colorless sulfur bacterium Beggiatoa leptomitoformis D-402 were revealed based on comprehensive analysis of its genomic organization, together with physiological, biochemical and molecular biological approaches. Strain D-402 was capable of aerobic methylotrophic growth with methanol as a sole source of carbon and energy and was not capable of methanotrophic growth because of the absence of genes of methane monooxygenases. It was established that methanol can be oxidized to CO2 in three consecutive stages. On the first stage methanol was oxidized to formaldehyde by the two PQQ (pyrroloquinolinequinone)-dependent methanol dehydrogenases (MDH): XoxF and Mdh2. Formaldehyde was further oxidized to formate via the tetrahydromethanopterin (H4MPT) pathway. And on the third stage formate was converted to CO2 by NAD+-dependent formate dehydrogenase Fdh2. Finally, it was established that endogenous CO2, formed as a result of methanol oxidation, was subsequently assimilated for anabolism through the Calvin–Benson–Bassham cycle. The similar way of one-carbon compounds utilization also exists in representatives of another freshwater Beggiatoa species—B. alba.

Draft Genome Sequence of Modestobacter sp. strain VKM Ac-2676 from soil in the area of sharply continental climate

A draft genome sequence of a novel actinobacterium, Modestobacter sp. strain VKM Ac-2676, was derived using Ion Torrent sequencing technology. The genome size is 3.88 Mb with average G+C content of 73.40%. The genes encoding properties relevant to adaptability of this bacterium to a sharply continental climate zone were revealed.

Draft Genome Sequence of Methylophaga muralis Bur 1, a Haloalkaliphilic (Non-Methane-Utilizing) Methylotroph Isolated from a Soda Lake

ABSTRACT The draft genome sequence of Methylophaga muralis strain Bur 1 (VKM B-3046T), a non-methane-utilizing methylotroph isolated from a soda lake, is reported here. Strain Bur 1 possesses genes for methanol and methylamine (methylamine dehydrogenase and N-methylglutamate pathway) oxidation. Genes for the biosynthesis of ectoine were also found.

Draft Genome Sequence of Methyloligella halotolerans С2T, a New Halotolerant Methylotroph, Accumulating Poly-3-Hydroxybutyrate and Ectoine

ABSTRACT Methyloligella halotolerans С2T is a moderate halophilic obligate methylotroph, accumulating ultra-high-molecular-weight poly-3-hydroxybutyrate (up to 8 to 10 MDa) from methanol. Here we report a draft genome and annotation of Methyloligella halotolerans C2T (VKM B-2706T = CCUG 61687T = DSM 25045T).