Sérgio Ferreira de Oliveira

Light and Electron Microscopic Analysis of Controlled Injury to Follicular Unit Grafts

BACKGROUND. Careful manipulation of hair units is essential for a good yield of transplanted hair. OBJECTIVE. To analyze the morphology of dissected follicular units submitted to crushing, stretching, bending, and drying. METHODS. Follicular units were either crushed, bent, stretched with forceps, or left drying on surgical gloves for 3 minutes. The specimens were fixed and prepared for observation with light microscopy or transmission and scanning electron microscopy. RESULTS. No alterations were detected in follicular units that had been crushed, bent, or stretched. Major damage occurred in samples that were left to dry on gloves. CONCLUSION. Letting the follicular unit dry appears to be the worst mishandling to which the follicular units may be subjected during routine hair transplantation.

Prognostic significance of silent myocardial ischemia after a first uncomplicated myocardial infarction

Forty asymptomatic patients were studied after a first uncomplicated myocardial infarction. They were 36 men and 4 women, with a mean age of 52.6 yr; the location of myocardial infarction was in the anterior wall in 18 (45%) patients and in the inferior wall in 22 (55%). The patients were submitted to: (1) 48-h Holter monitoring, during the 2nd and 8th weeks after the acute event; (2) exercise testing during the same periods; (3) cardiac catheterization and coronary arteriography. Patients with clinical conditions associated with cardiac rhythm disturbances or repolarization abnormalities were excluded. The electrocardiographic methods identified 11 (27.5%) patients with silent myocardial ischemia. Patients with and without silent ischemia were similar in relation to sex, age, coronary risk factors, arrhythmias, left ventricular function and follow-up. Patients with silent ischemia had more inferior wall myocardial infarctions, but the difference was not statistically significant. Patients with silent ischemia had significantly more extensive coronary artery disease (45.5% multivessel disease) when compared to those without ischemia (14.8% multivessel disease) (p < 0.05). After a 2-yr follow-up, 4 (36.4%) patients with and 1 (3.4%) without silent ischemia had a coronary event (p < 0.05). Kaplan-Meier analysis demonstrated a significantly higher cumulative probability of not experiencing a new coronary event for the patients without silent ischemia (96.5%) as compared to those with silent ischemia (62.3%) (p < 0.01). Our results suggest that silent myocardial ischemia after a first uncomplicated myocardial infarction carries an adverse prognosis and should be routinely investigated.

Organization of intermediate filaments and their association with collagen-containing phagosomes in mouse decidual cells

We have analyzed the distribution of intermediate filaments (IF) in the cytoplasm of mature decidual cells of mice. IF were scattered throughout the cytoplasm of these cells although there was a preferential accumulation around the nuclei. In many cells a large area of the cytoplasm was occupied by a rich network of IF that extended from the perinuclear region toward the cell surface. Thin bundles of IF crossed the cytoplasm without a preferential orientation. IF were also seen in close association with nuclear pore complexes, gap junctions, mitochondria, and lysosomes. A very developed network of IF surrounded phagosomes that contained collagen fibrils. Longitudinal and cross sections of these phagosomes showed a very close association of IF with the phagosome membrane.

Avaliação da prevalência do diabetes e da hiperglicemia de estresse no infarto agudo do miocárdio

OBJECTIVES: To evaluate in our population the real prevalence of diabetes (DM) and stress hyperglycemia (HE) in patients with myocardial infarction (IAM) admitted in a cardiologic emergency unit. METHODS: A retrospective analysis of 2262 patients with AMI evaluating the prevalence of DM (referred and diagnosed) and stress hyperglycemia. RESULTS: Besides 12,1% of subjects were previously referred to be diabetic (men: 10.7% and women: 15.8%), diabetes was effectively diagnosed in 24,8% (M: 22,9%, W: 29,7%) and stress hyperglycemia in 13,6% HE of the patients (M: 14,3%, W: 11,7%) indicating that glycemic alterations were effectively observed in 37.2.% of the patients with IAM (M: 37,2%, W: 41,4%). In DM subjects IAM events occurred earlier, total intra-hospital mortality was higher (DM: 20.7%, ND: 13,8%, HE: 13,4%) and less surgical procedures were performed (ND 33.8%, DM: 21.7%, HE: 18.0%). CONCLUSION: The elevated DM and stress hyperglycemia prevalence observed in our study indicates that glycemic alterations is one of the most important risk factors for IAM.

Ectoplacental cone induces resistance to apoptosis in high doses of interferon (IFN)-γ-treated decidual cells.

Citation Borbely AU, Fontenele‐Neto JD, Vidsiunas AK, Gomes SZ, Hoshida MS, de Oliveira SF, Bevilacqua E. Ectoplacental cone induces resistance to apoptosis in high doses of interferon (IFN)‐γ‐treated decidual cells. Am J Reprod Immunol 2012; 67: 73–83

Revascularização do miocárdio no paciente octogenário: 15 anos de observação

This is a retrospective study where 47 patients submitted to the conventional revascularization of the myocardium were analysed at InCor in a period covering January/78 to January/93; 47 patients were analysed. Thirty-tive (74.46%) were male and 12 (25.33%) female. The mean age was 81.85 (80-80) years. The operatory indication was due to unstable angina in 29 (61.70%), stable angina in 17 (36.17%) and dissection of the atheromatous plaque during angioplasty in 1 (2.12%). The operation was carried out eletively in 33 (70.21 %), in caracter of urgency in 10 (21.27%) and in emergency in 4 (8.51 %). All of them were operated on through median thoracotomy with extracorporeal circulation and moderate hypotermia. The saphenous vein was used as a graft in 41 (87.23%) patients and the internal thoracic artery in 6 (12.76%). Hospital mortality was 8.5% and the mean follow-up time was 17,6 months. From among the early and late deaths only one of each group was related to cardiopathy. Analysis of the cases revealed that in the period of 1991, 1992 and January/1993, hospital mortality fell down to zero. Surgical conventional revascularization of the myocardium represents a good alternative for the octogenarian patient, not only due to the present operative low risk, but as well as the reduction or elimination of the symptoms during the late follow-up.

Revascularização do miocárdio no idoso: fatores de risco para morbidade e mortalidade hospitalar

Com a finalidade de determinar os principais fatores de risco para a morbidade e mortalidade hospitalar de pacientes coronarianos idosos (idade > 65 anos), submetidos a revascularizacao isolada, eletiva do miocardio, estudamos prospectivamente 72 pacientes consecutivos, que possuiam observacoes completas no InCor, no periodo compreendido entre janeiro e dezembro de 1988. No roteiro do protocolo foram incluidos fatores clinicos, radiologicos, hemodinâmicos, operatorios e de pos-operatorios (Tabela 1). Os resultados foram analisados utilizando-se o teste do Qui quadrado de Pearson e a Prova Exata de Fisher, atraves do SPSS (Statistical Package for Social Science). Dentre os fatores analisados, apresentaram significância estatistica: o tabagismo, o numero de vasos coronarios acometidos, o tempo de duracao da circulacao extracorporea, o tempo de pincamento da aorta, o numero total de enxertos realizados, a presenca de anastomose mamaria coronaria e o indice de movimentacao da parede ventricular esquerda.