Sérgio José de Oliveira

Isolamento de Salmonella Braenderup em arara-azul (Anodorhynchus hyacinthinus)

The sanitary monitoring of free-living Hyacinth macaw (Anodorhynchus hyacinthinus) allows adjusting the management in altered natural habitat. To evaluate the occurrence of Salmonella spp. it was collected cloacal swabs of this nestlings species, in the Pantanal wetlands, in the state of Mato Grosso do Sul, Brazil. One Salmonella-like colony was serologically typed and identified as Salmonella Braenderup. Due to the high zoonotical potential of this microorganism, it is important an effective sanitary control of wildlife psittacines. In the literature searched it was not found any report on isolation of this bacterium in Hyacinth macaw for both free-living and captive animals.

Phenotypic and molecular characterization of recent and archived Erysipelothrix spp. isolated from Brazilian swine

One hundred fifty-one Erysipelothrix spp. isolates from Brazilian swine were characterized by serotyping, determination of antimicrobial susceptibility, amplified fragment length polymorphism (AFLP), and pulsed-field gel electrophoresis (PFGE). Among all isolates, 139 were classified in 18 different serotypes and serotype 2b was the most frequent. The susceptibility profiles of the isolates were very similar among each other, which did not permit subtyping Erysipelothrix spp. isolates by the antimicrobial susceptibility testing. Despite the fact that AFLP and PFGE provided the same discriminatory index (0.98), PFGE was more discriminatory than AFLP, given the types of groups it generates. Regardless the technique employed (AFLP or PFGE), no discrimination between recent and historical isolates was established, neither a fixed epidemiologic pattern for their grouping was observed. Nevertheless, AFLP could be an interesting alternative for discriminating the Erysipelothrix species, while PFGE could be an indication for discerning this bacterium according to the serotypes.

Genotyping of Brazilian Erysipelothrix spp. strains by amplified fragment length polymorphism

One hundred and fifty-one Erysipelothrix spp. isolates from diseased and carrier swine from Brazil were identified by PCR, submitted to serotyping and analyzed by amplified fragment length polymorphism with a single enzyme (AFLP). Reference strains from Australia and the United Kingdom were also examined. The 151 strains were classified into 18 different serotypes (1a, 1b, 2a, 2b, 4, 5, 6, 7, 8, 10, 11, 12, 15, 17, 19, 21, 24 and 25), being serotype 2b the most frequent (39.7%). By associating serotyping and PCR results, it was possible to identify 146 strains as E. rhusiopathiae and five strains as E. tonsillarum. Despite the fact that for this genus AFLP did not cluster all isolates according to serotype, origin, disease or isolation data, the execution of the technique was easy and fast, demonstrating high discriminatory power. The results produced by the AFLP analysis of Erysipelothrix spp. could also support its use as a discriminatory tool for E. rhusiopathiae and E. tonsillarum species.

Listeriose em rebanho de ovinos leiteiros na região serrana do Rio Grande do Sul: relato de caso

Clinical cases of listeriosis in two Lacaune rams, fed silage in a lot, are reported. Histological examination of nervous tissue revealed purulent meningoencephalitis, and L. monocytogenes was detected in nervous tissue by immunohistochemistry procedures. This disease should be suspected when oral lesions are visible in dairy sheep fed low quality silage.

Isolation of Arcobacter spp from poultry carcasses, in Brazil

Fourty eight isolates of Arcobacter spp were obtained from 37 poultry carcasses, from abattoir, among 80 carcasses examined. Attempts for culturing were made from the skin and muscle, resulting on 25 positive cultures from muscle and 23 from skin. Classification was achieved by phenotypic characterization and PCR and multiplex PCR, resulting 41 samples of Arcobacter butzleri and 07 Arcobacter sp. This is the first report on the occurrence of Arcobacter in animal carcasses in Brazil.

EVIDÊNCIAS DE INFECÇÃO POR LEPTOSPIRA BRATISLAVA EM TRANSTORNOS REPRODUTIVOS EM SUÍNOS

SUMMARY Sera from 535 sows originated from 25 swine herds, were tested through the Microscopic Agglutination Test (MAT). In these herds there were reports of abortions, stillbirths and returns to service. Leptospirosis was suspected. Leptospiral antibodies were shown on 113 serum samples. Leptospira bratislava titres were present in 42 sera, L. icterohaemorrhagiae in 39 sera. Cross reactivity was found with bratislava and icterohaemorrhagiae antigens in 20 serum samples. Present results showed different serologic patterns of Leptospirosis in pigs in Brazil. L. poaona titres had been the most prevalent, in previous reports. Evidence of infection by L. bratislava has not previously been reported in pigs in Brazil.

Isolamento de Arcobacter butzleri de músculos de carcaças de suínos de terminação e de matrizes descartadas abatidos em um matadouro no Estado do Rio Grande do Sul, Brasil

Portions of muscle from carcasses of 74 fattening pigs and 150 culled sows were collected from abattoir in the State of Rio Grande do Sul, Brazil. The samples were inoculated into EMJH (Ellinghausen, MacCullough, Johnson & Harris) medium and the culture filtered through 0.45µm membrane on blood agar. Arcobacter sp were isolated, 69 isolates, typed by PCR as Arcobater butzleri, respectively from fattening pigs (21 isolates, 29.3%) and culled sows (48 isolates, 32%).

Isolamento de Aerobacter (Campylobacter) cryaerophila de fetos suínos abortados

Foram examinados 6 fetos suinos abortados no terco final da gestacao, provenientes de duas granjas do Rio Grande do Sul, Brasil. Os orgaos internos (rim e figado) de cada feto foram inoculados em meio semi-solido de Tween 80/40 LH, visando o isolamento de leptospiras. Os tubos foram incubados a 30°C. Foi isolado de todos os materiais um microorganismo, classificado como Arcobacter cryaerophila. Estes foram os primeiros isolamentos desta bacteria no Brasil.

Diversidade antigênica entre amostras de Arcobacter spp isoladas de suínos no Rio Grande do Sul e presença de anticorpos aglutinantes em amostras de soro de porcas com problemas reprodutivos

Microscopic Agglutination Test (MAT) described as a diagnostic test for Leptospirosis was used to check antigenicity on 47 samples of Arcobacter cryaerophilus and two samples of Arcobacter butzleri isolated from pigs in Rio Grande do Sul, Brazil. Rabbit antisera used in the test were produced against reference strains of Arcobacter spp. Antigenic heterogenicity among the samples was shown. Only four samples provoked titres higher than 1.600 with standard antisera. 48.97% of the antigens confirmed the genetic classification of the microrganisms. MAT was used also to identify serum samples from sows with reproductive problems from farms where Arcobacter spp had been isolated. There is no reference in the literature on the use of the test as diagnostic tool in Arcobacter infections. Serologic tests in sows showed titres as high as 1,600, suggesting that there was presence of agglutinins in serum samples.

Bacteriological analysis of pulmonary adherence in slaughter pigs from different origins at an abattoir in Rio Grande do Sul, Brazil

1 Medico Veterinario Residente, Laboratorio de Bacteriologia e Micologia, Hospital Veterinario, ULBRA, Canoas, RS; 2 Medico Veterinario, Servico de Inspecao Federal – SIF, Lajeado, RS; 3 Medico Veterinario, Perdigao Alimentos, Lajeado, RS; 4 Medico Veterinario autonomo, RS 5 Medico Veterinario, Prof. Dr., Laboratorio de Bacteriologia e Micologia, Hospital Veterinario, Curso de Medicina Veterinaria, ULBRA, Avenida Farroupilha 8001,Canoas, RS, 92425-900. serjol@terra.com.br ______________________________________________________________________________________ Resumo: Foram realizados exames bacteriologicos em 123 amostras de pulmoes em casos de aderencia as carcacas, no abate de 59 lotes de suinos em um frigorifico no Rio Grande do Sul. Apos a classificacao das lesoes pelo Servico de Inspecao Federal, foram colhidas 49 amostras de pulmoes de carcacas liberadas para consumo, 45 com destino para conserva e 29 de carcacas que foram descartadas para graxaria. Atraves dos exames bacteriologicos verificou-se maior frequencia de isolamentos de Pasteurella multocida (61 amostras) e Arcanobacterium pyogenes (52 amostras), ocorrendo tambem coinfeccoes com estas bacterias. 44 isolados de P. multocida foram classificadas como tipo capsular A. Outras bacterias isoladas com menor frequencia foram Actinobacillus pleuropneumoniae (4 isolamentos), Haemophilus parasuis (2), Streptococcus suis (5), Streptococcus beta hemolitico (4), Nocardia sp (3), Aeromonas sp (1), Staphylococcus sp (1) e 20 materiais com resultado negativo ao exame bacteriologico. As amostras de P. multocida revelaram muita suscetibilidade aos antimicrobianos em teste de difusao, ocorrendo apenas duas cepas resistentes a oxitetraciclina, uma resistente e tres intermediarias para estreptomicina e uma resistente e outra intermediaria a sulfa-trimetoprima. Termos de indexacao: exame bacteriologico, pulmoes, suinos de abate