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Featured researches published by Seri Koonjaenak.


Italian Journal of Animal Science | 2007

Assessment of semen quality in Swamp Buffalo AI Bulls in Thailand

Seri Koonjaenak; Heriberto Rodriguez-Martinez

Abstract Characteristic of Thai swamp buffalo bulls semen used for artificial insemination (AI) in Thailand, aspects relevance in freezing and thawing of semen are review. Semen and sperm characteristics were evaluated included sperm count, motility (assessed subjectively and by CASA), morphology (using phase-contrast light microscopy and SEM), plasma membrane integrity (PMI) (using a hypo-osmotic swelling test [HOST]) and SYBR-14/propidium iodide [PI]), plasma membrane stability (PMS) (using Annexin-V/PI) and deoxyribonucleic acid (DNA) integrity (using SCSA and flow cytometry [FCM]). The average ejaculate volume was about 3.0–4.0 mL, with good viability (PMI measured by the HOST) and motility (<65% and <70%, respectively). Sperm concentration ranged from 1.1 to 1.2 billion/mL, being also affected by bull age. Whereas semen quality (including sperm output, pH and initial sperm motility) did not differ between the seasons. Few spermatozoa (>15%/ ejaculate) had abnormal morphology with abnormalities resembling those in other bovidae. In FT semen, PMI (using SYBR-14/PI) and PMS were highest in winter. Across seasons, ~50% of post-thaw spermatozoa depicted linear motility, a proportion that decreased to ~35% during incubation (38oC for 60 minutes), without marking any seasonal difference. The sperm DNA was hardly damaged (with <3% fragmentation, expressed as DNA fragmentation index [DFI]), among seasons.


Theriogenology | 2007

Seasonality affects post-thaw plasma membrane intactness and sperm velocities in spermatozoa from Thai AI swamp buffaloes (Bubalus bubalis)

Seri Koonjaenak; P. Pongpeng; S. Wirojwuthikul; A. Johannisson; Annop Kunavongkrit; Heriberto Rodriguez-Martinez


Asian Journal of Andrology | 2007

Seasonal variation in semen quality of swamp buffalo bulls (Bubalus bubalis) in Thailand.

Seri Koonjaenak; Vichai Chanatinart; Suneerat Aiumlamai; Tanu Pinyopumimintr; Heriberto Rodriguez-Martinez


Journal of Veterinary Medicine Series A-physiology Pathology Clinical Medicine | 2007

SEASONAL VARIATION IN NUCLEAR DNA INTEGRITY OF FROZEN-THAWED SPERMATOZOA FROM THAI AI SWAMP BUFFALOES (BUBALUS BUBALIS)

Seri Koonjaenak; A. Johannisson; P. Pongpeng; S. Wirojwuthikul; Annop Kunavongkrit; Heriberto Rodriguez-Martinez


Journal of Veterinary Medicine Series A-physiology Pathology Clinical Medicine | 2007

Morphological Features of Spermatozoa of Swamp Buffalo AI Bulls in Thailand

Seri Koonjaenak; V. Chanatinart; Hans Ekwall; Heriberto Rodriguez-Martinez


Kasetsart Journal. Natural Sciences | 2010

Backfat Thickness at First Insemination Affects Litter Size at Birth of the First Parity Sows

Atthaporn Roongsitthichai; Seri Koonjaenak; Padet Tummaruk


Comparative Haematology International | 2011

Association between serum cortisol and progesterone concentrations and the infiltration of immune cells in the endometrium of gilts with vaginal discharge

Atthaporn Roongsitthichai; Junpen Suwimonteerabutr; Kampon Kaeoket; Seri Koonjaenak; Padet Tummaruk


Thai Journal of Veterinary Medicine | 2010

The Use of Computer-Assisted Sperm Analysis for Discriminating Series of Motility Pattern of Frozen-thawed Boar Semen

Chanyuth Tretipskul; K. Buranaamnuay; Seri Koonjaenak; Padet Tummaruk; Mongkol Techakumphu


Archive | 2011

Association between Backfat Depth and Litter Size at Birth in Primiparous Sows

Atthaporn Roongsitthichai; Seri Koonjaenak; Padet Tummaruk


Warasan Sattawaphaet | 2002

Histological and histochemical studies on esophagus of the germain's swiftlet (Collocalia germani Oustalet, 1878)

Dollada Srisai; Sahathep Juntaravimol; Pakawadee Pongkete; Seri Koonjaenak; Apinun Suprasert

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Padet Tummaruk

Chulalongkorn University

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A. Johannisson

Swedish University of Agricultural Sciences

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