Serpil Bozkurt

Comparison of uterine artery blood flow with levonorgestrel intrauterine system and copper intrauterine device

BACKGROUND As a contraceptive method, we investigated whether the levonorgestrel-releasing intrauterine system (LNG-IUS) has any effect on uterine artery blood flow when compared with copper intrauterine device (IUD). STUDY DESIGN Sixty women with copper IUD and 60 women with LNG-IUS were included in the study. The age, gravidity, parity, body mass index (BMI) and menstrual flow pattern using a pictorial chart were recorded. All women were assessed by ultrasonography at the preinsertion period and 1 year after insertion. The pulsatility and resistance indices (PI and RI, respectively) of the uterine artery and endometrial thickness were evaluated in the preinsertion and postinsertion periods. RESULTS In copper IUD users, preinsertion and postinsertion ultrasonographic assessments were not significantly different. However, postinsertion RI was significantly higher compared with preinsertion RI in LNG-IUS users (p=.001). The PI was also increased 1 year after insertion, but it did not reach statistically significant levels (p=.08). Endometrial thickness was also significantly decreased in the postinsertion period in women with LNG-IUS (p=.04). CONCLUSION The significant increase in uterine artery RI in LNG-IUS users 1 year after insertion might be due to its local progestational effects. It might also indicate the mechanism of the LNG-IUS in reducing menstrual blood flow.

Comparison of the acute alterations in serum bone turnover markers and bone mineral density among women with surgical menopause

OBJECTIVE To determine the effect of a sudden decrease in estrogen levels via bilateral oophorectomy on serum turnover markers and to examine their correlation with bone mineral density (BMD). STUDY DESIGN This study included 51 women who had regular menses preoperatively and underwent bilateral oophorectomy for benign reasons. These women did not have any systemic disease or drug use that would influence bone metabolism. For each woman, spine and femur BMD were measured preoperatively and sixth months after surgery. Serum C-terminal telopeptide (CTX) and bone alkaline phosphatase (BAP) were measured preoperatively and at the first and sixth months after surgery. Correlations between bone turnover markers and BMD were pre- and post-operatively analyzed. RESULTS The mean serum CTX and BAP concentrations at the first and sixth postoperative months were significantly higher compared to the preoperative measurements (p=0.001). Spine BMD values at the sixth postoperative month was significantly lower compared to preoperative period (p=0.0001). There was a significant negative correlation between spine BMD values and BAP levels both in the preoperative period and at the sixth postoperative month (r=-0.407, p=0.001), whereas a significant positive correlation between serum CTX and BAP was noted at this time periods (r=0.615, p<0.001). CONCLUSIONS The results of this study showed that serum BAP and CTX levels rapidly increase in women after bilateral oophorectomy. Therefore, these markers (especially BAP) could be useful in the evaluation of osteoporosis risk in the early period of surgical menopause.

Laparoscopic surgery of interstitial (cornual) pregnancy, a case report

We report a successful laparoscopic management of an interstitial pregnancy of a 24- year-old single woman, treated by cornuostomy. The patient was first managed with methotrexate treatment. After the 2. methotrexate administration, the patient suffered from low abdominal pain, and intraabdominal bleeding signs were reported by transvaginal ultrasonograpy. The hemoglobin level was decreased from 12.8 gr/dl to 11.8 gr/dl and the beta hCG level was increased from 8,314 mIU/l to 11,541 mIU/l. The laparoscopic approach to interstitial pregnancy was presented and other management strategies such as medical treatment and laparotomy have been reviewed.

Fluid intake and voiding parameters in asymptomatic Turkish women

Introduction and hypothesisFor an accurate evaluation of bladder diaries, we aim to investigate normal urinary habits and determining factors on functional bladder capacity, frequency, and 24-h volume in the bladder diaries of asymptomatic women.MethodsOne-hundred and fifteen asymptomatic women who recorded a 24-h bladder diary were included in the study. Linear regression analyses were used to explore associations between diary values and patient characteristics.ResultsTotal number of voids was related to age, body mass index, total voided volume, total fluid intake, total diuresis rate, and maximum fluid intake in one go. Maximum, average, and minimum volumes per void were found to be related to body mass index, total voided volume, total fluid intake, total diuresis rate, and maximum fluid intake. When we used multiple regression analysis, only maximum fluid intake was found to be related to the total number of voids, maximum, average, and minimum volumes per void.ConclusionsMaximum fluid intake rather than total voided volume seems to be an important determinant factor for total number of voids and functional bladder capacity.

Report of surgical correction of a cervicovaginal agenesis case: cervicovaginal reconstruction with pudendal thigh flaps

Congenital agenesis of the uterine cervix and vagina in the presence of a functional endometrium is an extremely rare mullerian anomaly [1]. Congenital vaginal agenesis occurs with a prevalence of 1/4000-1/5000 live-born females, while the accurate incidence of cervicovaginal agenesis is controversial [1–3]. Cervicovaginal agenesis is usually not diagnosed until menarche. It is manifested by the collection of menstrual blood in the functional endometrium, Fallopian tubes and peritoneal cavity, leading to primary amenorrhea and cyclic abdominal pain [1, 4]. The primary goal of cervicovaginal reconstruction in cervicovaginal agenesis cases is to preserve reproductive performance [5–8]. In routine clinical practice, hysterectomy was advised in cases with complete cervical aplasia, to control pain and dysmenorrhea and also prevent complications such as peritonitis [4, 9, 10]. But recent studies are more encouraging [3]. However, as different techniques were performed for each individual type of cervical agenesis case, there is great controversy as to the best technique because each has associated advantages and disadvantages. In this case report, a functional reconstruction with pudendal thigh flaps complicated with endometrioma and hematosalpinx in a cervicovaginal agenesis case will be discussed in the light of the literature. An 18-year-old virgin woman with a history of primary amenorrhea and cyclic abdominal pain was referred to our gynecology clinic with normal secondary sex characteristics. Adrenarche and thelarche had occurred by age 13 years. Physical examination revealed no abnormalities. The vagina ended with a 1 cm blind pouch. A pelvic sonographic examination showed an abnormally enlarged uterus due to hematometra and also a left ovarian endometrioma. No cervical tissue was observed in inspection or sonographic imaging, indicating complete cervical agenesis. The intravenous pyelogram showed a normal urinary tract system. Magnetic resonance imaging (MRI) revealed an arcuate uterus and a normal endometrial cavity with complete cervical agenesis (Figure 1). The laboratory work-up showed normal serum levels of follicle-stimulating hormone, thyroid-stimulating hormone, and prolactin. The karyotype was 46, XX. The patient was informed about the procedure and informed consent was obtained. Figure 1 Transverse MRI view of the arcuate uterus Bilateral posterior neurovascular pudendal thigh flaps were planned. The course of the superficial perineal artery and the flap dimensions (approximately 11 cm × 3.5 cm) were marked. The procedure was performed with a semilithotomy position allowing both abdominal and perineal approaches. A medial vertical incision parallel to the groin crease was made. A lateral vertical incision was placed, depending on the necessary size of the flap (8 cm width in our case). During dissection, the deep fascia of the thigh and epimysium of the muscles were included with the flaps (Figure 2). An incision of 1.5 cm diameter was made on the uterine fundus with a 10-mm dilator via the abdominal approach (Figure 3). This maneuver helped to identify the upper limit of the atretic tissue of the cervix. The atretic cervical tissue was resected and a blunt dissection was performed between the bladder and the rectum to form a normal vaginal pouch. After the primary coverage of the donor sites, the flaps were then delivered medially through a channel between the pivot point (posterior of the flaps) and the neovaginal area. Figure 2 Creation of utero-vaginal anastomosis via 10 mm dilator Figure 3 Distal aspect of the pudendal thigh flap with anterior abdominal wall The flaps were conjoined with polyglycolic acid sutures at anterior and posterior zones of the neovagina. The apexes of the combined flaps were sutured to the uterus with stitches of 2-0 polyglactin. The left ovarian endometrioma was excised. The bilateral closed fimbrial ends were also opened by fimbrioplasty. A 16 CH Foley catheter was inserted into the uterine cavity for 20 days. The cavity was irrigated with saline and 10% povidone-iodine during this period. Antibiotic prophylaxis (ampicillin) was maintained for 14 days. Low-molecular-weight heparin prophylaxis was administered for 7 days. No mold was required. There were no complications in the early postoperative period. The patient was discharged on the 20th day with no complications. The patient was assessed postoperatively at 1, 3, 6 and 12 months. The intrauterine catheter was removed and a Lippes loop intrauterine device (IUD) was inserted at a 1-month medical visit. The IUD was placed as a stent to maintain utero-vaginal anastomosis. The US examination was within normal limits. Vaginal depth was 8 cm. Menstruation was restored with a delay of 1 month postoperatively. At the 12th month examination, the IUD was removed and hysterosalpingography examination was performed. Hysterosalpingography revealed patent vaginal and cervical canals (Figure 4). Figure 4 Postoperative appearance of tubal patency by hysterosalpingography Cervical agenesis is usually seen with partial or complete vaginal agenesis. Since it is an uncommon anomaly, there is still no consensus about the treatment. First line treatment in reconstruction of agenesis can be conducted by repetitive dilatation and expansions [5]. However, in these techniques, multiple stages are necessary. In cervicovaginal agenesis reconstruction, the most commonly used technique has been cylindrical stent assisted, split-thickness skin graft, described by McIndoe and Banister [11]. Stenosis at the proximal one third of the vagina is a disadvantage of this method, which is seen in 21-42% of cases [12, 13]. The labial pocket flap was expanded using labia minora flaps [14] and a tissue expander [15]; these are the first ones to come to mind amongst the local-regional flaps in agenesis reconstruction. Since the tissue provided is limited, the use of these local flaps is limited in complete agenesis cases. The most important disadvantage of musculocutaneous flaps such as gracilis [16] and rectus abdominis [17] is that they cause bulky tissue formation. Some pediatric surgeons and urologists utilize a segment of bowel such as ileum, cecum, jejunum, sigmoid colon or peritoneum to create a vagina [12, 18, 19]. The advantages of this technique are its low contraction risk, elimination of the need for molds, providing lubrication and resistance against traumas [12]. However, this technique requires a laparotomy and bowel reanastomosis. Fedele modified the Vecchietti technique and described laparoscopy assisted uterovestibular anastomosis [20]. Fedele et al. reported regular menstrual bleeding for all 12 patients in their series via this technique [21]. They mentioned that there was no need of vaginal molding after surgery. But they recommended inhibition of bleeding from functioning endometrium with gonadotropin-releasing hormone analogs. The advantages of laparoscopic access are shorter postoperative stay, less postoperative pain and no laparotomy scar. However, long-term vaginal molding was needed after some laparoscopic interventions [22]. Vecchiettis procedure requires special care in the graduation of the threads’ tension. Oral analgesia was usually needed for pain control during the procedure. The pudendal thigh flap used in our case is an easily prepared fasciocutaneous flap. It is a skin flap based on the groin crease, first described by Wee and Joseph [23]. It has been frequently utilized for perineal and vaginal reconstruction and recto- or vesico-vaginal fistula repair, as it has been described as easy to harvest, thin, pliable and sensate [24, 25]. Since it is a vascular tissue, it is superior to a skin graft (McIndoe method) or other grafts. It minimizes the risk of contraction or stenosis in the flaps. The main advantage of our technique is that there is no need to use long-term postoperative molds, dilators, or obturators to avoid stenosis or contraction of the vagina and the donor site scars are well hidden under the pubic line. When a functional result such as pregnancy is considered, it is not possible to absolutely state which of these operative techniques are superior. As the condition is rare, the ability to perform long-term follow-up studies comparing the various options is limited. The most frequently used method has been the McIndoe technique [7, 26]. When compared to the McIndoe technique, which has a high rate of stenosis [12], a higher chance of pregnancy with flaps can be stated. In the 1-year follow-up of our case, uterovaginal opening was achieved, functional endometrium was protected and partial tubal passage was present. The findings were confirmed by hysterosalpingography. The most important disadvantage of the pudendal thigh flap is hirsutism. It is reported that the mucosa which is maintained by the McIndoe technique is much more similar to normal vaginal mucosa. In cases of insufficient atrophy, postoperative depilation may be necessary [10]. In our case, increased hair growth was observed during the 1- and 3-month follow-up with a decrease in the 6-month follow-up. At the end of the postoperative 1st year, hirsutism had almost completely disappeared. In conclusion, various techniques in construction of the vagina and cervix agenesis have been described in the literature. Reconstruction cases using thigh flaps are very few and the efficacy of the technique for pregnancy is still not clear. However, it should be kept in mind as a simple technique in cervicovaginal agenesis reconstruction which eliminates the need for complex surgical processes.

Comparison of serum androgens and endometrial thickness in obese and non-obese postmenopausal women

OBJECTIVE In this study, we investigated whether serum androgen levels and endometrial thickness differed in obese and non-obese women. MATERIAL AND METHODS Thirtytwo non-obese (BMI <30) and 48 obese (BMI ≥ 30) women were enrolled. Blood samples were analyzed for testosterone, free testosterone, androstenedione, DHEAS, and SHBG, and transvaginal ultrasonography was performed. RESULTS Obese women had significantly higher free testosterone and endometrial thickness and significantly lower SHBG. Eight of 17 women with endometrial thickness >5 mm had significant pathology. CONCLUSION These results suggest that obesity may be a risk factor for endometrial carcinoma and other pathologies in post-menopausal women through an action on androgen concentrations.