Setyo Widodo

Concentration of Calcium and Phosphate Serum Following Administration of Zinc in Friesian Holstein Bulls

Minerals play an important role in bulls as they function to form mucles of and maintain the quality of sperm. Zn mineral in the diet will also affect the levels of calcium and phosphorus in the blood. The objective of this study was to determine the effect of Zn supplementation on the levels of calcium and phosphate serum in Friesian Holstein bulls. This study used ten Friesian Holstein bulls, 16-18 months old and divided into two groups, consisting of five bulls each, i.e. with no added Zn (control) and 60 ppm of Zn supplementation, respectively. Zinc was administered daily for four months. Blood samples were taken before treatment and after the four- month treatment for the examination of calcium and phosphate levels. The result showed that 60 ppm Zn supplementation was not significantly different (p>0.05) on the blood calcium and phosphate levels. However, the calcium level tends to increase while that of phosphate tends to decrease.

Profil Imunoglobulin-G Serum Kambing Peranakan Etawah Bunting yang Diberi Imbuhan Pakan Mineral Seng (SERUM IMUNOGLOBULIN-G LEVEL ON PREGNANT ETTAWAH CROSSBRED WERE GIVEN ZINC MINERAL)

The objective of this study was to find out the effect of zinc supplementation on the profile of immunoglobulin-G in the pregnant etawah-cross ewes. Fifteen etawah-cross ewes (3-6 years old and 3050 kg body weight) were used in this experiment and they were divided into three groups each of which consisted of 5ewes. The first group (Zn40) received 40 ppm Zn as control, the second group (Zn60) received 60 ppm Zn, and the third group received 80 ppm Zn. Blood samples were collected from jugular vein every two weeks, started at twelve weeks of pregnancy up to eight weeks post partum for immunoglobulin-G analysis. IgG level were analyzed by competitive Enzyme Linked Immunosorbent Assay (ELISA) technique. Results showed that there were no significant difference of immunoglobulin-G level (P>0,05) among the three treatment groups ( Zn40, Zn60, and Zn80). However, IgG level of Zn40 and Zn80 groups tended to decrease during the last staget of pregnancy until two weeks post partum. In conclusion, supplementation of 60 ppm Zn showed higher IgG level than Zn 80 ppm.