Seung-Ki Kim

Clinical application of sentinel lymph node biopsy based on axillary anatomy in breast cancer: A single institution experience.

BACKGROUND Sentinel lymph node biopsy (SLNB), mostly with the use of vital dye or radioisotope, is a method for predicting axillary status in patients with breast cancer. Conventional axillary lymph node dissection (ALND) is used in cases where sentinel lymph node (SLN) is not detected by existing methods, but a series of studies have found that most of SLNs are present in specific anatomical spaces. We attempted to determine the feasibility of SLNB based on axillary anatomy in cases where SLN was not detected by conventional lymphatic mapping methods. METHODS A retrospective analysis involving 208 patients who received anatomical SLNB between January 2003 and December 2010 was performed. Lateral border of the pectoralis major muscle and lateral thoracic vein were defined as the anatomical landmarks, and ALND was performed to at least level II, regardless of the results of frozen section analysis. Pathologic results were used to measure false negative rate and accuracy. Chi-square test and Fishers exact test were performed to find factors affecting results. RESULTS False negative rate and accuracy of anatomical SLNB were 21.7% (13/60) and 93.3% (182/195), respectively. T stage, clinical node status, number of dissected SLNs and body mass index were analyzed as factors affecting results, but none of them was found as having a statistically significant influence. CONCLUSION These results suggest that anatomical SLNB may not replace ALND in cases where SLN is not detected by conventional lymphatic mapping method, but may be considered as a method for predicting axillary status before conducting a node dissection.

P109 Idiopathic granulomatous mastitis; a case series

compounds with anticancer and antitumor activity. Onchidium is an airbreathing and shell-less sea slug from gastropod mollusc in the family Onchidiidae, that is distributed with a good abundance in Persian Gulf. Methods: Adult Onchidium were collected from Persian Gulf during spring of 2010. Eggs were freeze dried and used for protein extraction by homogenization in acid acetic (0.1N). After centrifugation, supernatants were freeze dried. Freeze dried extract dissolved in ammonium acetate buffer and it was subjected to gel filtration on a Sephadex G50 column (1.4×60). Purified molecules were separated by SDS-PAGE under denaturing conditions in 12.5% polyacrylamide gels which was calibrated with SDS-PAGE polypeptide high range standards. A 320 kDa protein separated and their in vitro anti-proliferative and cytotxic effects on human breast cancer cell line (T47D in RPMI-1640 culture medium) examined by MTT assay. Results: This protein showed growth inhibitory activity against T47D cells with LC50 values ranging 0.240 to 0.923 nM. This result suggests that separated 320 kDa protein has potent activity against breast cancer cell line. To understand the mechanism of their anti-cancer action, the effect of this compound on the location of cells in the cell cycle was examined using fluorescent activated cell-sorting analysis. This protein was added to T47D culture medium at varying doses, and then at 24 hr the cells were examined for cell cycle location. The addition of separated 320 kDa protein to these cells caused a dose-dependent block in the cell cycle in the G2/M phase. Conclusion: Although these primeval results showed anti-proliferative capacity of this protein, the modulation of apoptotic pathways deserves further evaluation as an anticancer strategy. Disclosure of Interest: None Declared