Seymen Bora

Comparison of different surgical repairs in the treatment of experimental duodenal injuries

BACKGROUND In this experimental study, we aimed to investigate the results of different surgical repair methods for delayed reconstruction of severe experimental duodenal defects. METHODS A large duodenal defect with irregular and tagged margins covering about 50% of the circumference was created in the second part of duodenum of male Wistar rats. The effectiveness of primary repair, jejunal serosal patch, Roux-en-Y duodenojejunostomy, or expanded polytetrafluoroethylene patch repair techniques were investigated on the basis of survival and histologic assessment. RESULTS No significant survival benefit was observed between jejunal serosal patch, Roux-en-Y duodenojejunostomy, or expanded polytetrafluoroethylene patch repair techniques. But these repair modalities were associated with better survival rates than no-treatment or primary repair techniques. Complete coverage of the expanded polytetrafluoroethylene grafts by neomucosa consisting of columnar epithelium with villus formation was observed in surviving rats about 16 weeks after surgery. CONCLUSIONS Expanded polytetrafluoroethylene patch can be used in the repair of experimental large duodenal defects, which can not be repaired primarily.

Inflammatory fibroid polyp presenting with intestinal invagination: Sonographic and correlative imaging findings

We report a case of a large inflammatory fibroid polyp of the small bowel presenting with intussusception in a 22‐year‐old man. Sonography demonstrated a solid, mobile, homogeneous, echogenic mass surrounded by the typical mural layers of an invaginated ileum. CT demonstrated a well‐defined intraluminal solid mass with an attenuation of 17 HU. The pathologic diagnosis after segmental ileal resection was ileal inflammatory fibroid polyp.

The effects of vitamin E on immune regulation after thermal injury

Studies were conducted on four groups of rats, each group consisting of 10 rats with burn + vitamin E, burn + saline solution, control + vitamin E and control + saline solution. Before and after burning 250 mg (2 cm3) of vitamin E was given intramuscularly. On the same days 2 cm3 of saline solution was given to the controls. The rats were burned over 30 per cent of their body surface area and then inoculated intraperitoneally with 40 units of tetanus toxoid. Complement fixation and acid phosphatase tests were carried out on the days 15 and 21 postburn. On day 21, the rats were killed and the spleens removed and weighted. In the groups that received vitamin E, the spleen weight and complement fixation test increased significantly while the acid phosphatase in serum decreased. In the clinical study, 17 burned patients with over 20 per cent deep partial or full skin thickness burns and eight healthy persons were studied. Nine of the 17 burned patients received vitamin E on 3 consecutive days, on day 4 blood was taken for analysis. The results showed that the number of T-cells decreased significantly in burn patients (P less than 0.05) whereas they increased significantly (P less than 0.05) in burn patients who received vitamin E. It is concluded from these experimental and clinical studies that vitamin E stimulates both cellular and humoral immunity. Therefore, the use of vitamin E in combination with conventional therapy in burn patients can be recommended.

Seroma Prevention by Using Corynebacterium parvum in a Rat Mastectomy Model

Seroma formation is the most common complication after mastectomy and continues to be an important problem during the early postoperative period. Several surgical and medical methods have been developed to try to overcome this problem; however, so far none have been used successfully in the routine clinical practice. The aim of this study is to evaluate the effects of Corynebacterium parvum (CP) as a sclerosing agent in both prevention and treatment of seromas after mastectomy and axillary dissection in an animal model. Sixty female Sprague-Dawley rats underwent mastectomy and axillary dissection under general anaesthesia. Following surgery, the rats were treated in 1 of 3 ways. In the prevention group, 1 cm3 (0.35 mg) CP solution was injected beneath the skin flap just before closure of the incision after mastectomy. In the treatment group, animals in which a seroma was formed, the fluid was aspirated, and 1 cm3 CP solution was injected beneath the flap. In the control group, animals in which seromas formed, aspiration only was performed. The frequency of seroma formation decreased when CP solution was injected immediately after the operation (p < 0.01). In addition, seroma formation was effectively treated by CP injection when compared with the control group (p < 0.05). CP was effective as a prophylactic agent in the prevention group and as a therapeutic agent in the treatment group in this experimental model. CP injection may be useful for the management of this problem in a clinical setting.

Malignant islet cell tumor of the pancreas associated with portal venous thrombus.

Abstract. In this report, CT and MR findings of a malignant islet cell tumor of the pancreas associated with tumor thrombus in the portal vein is presented. Imaging findings revealed diffuse involvement of the body and tail of the pancreas by the tumor. The most unusual finding was that this invasive tumor was an insulinoma.

What is the role of the abdominal perfusion pressure for subclinical hepatic dysfunction in laparoscopic cholecystectomy

BACKGROUND Subclinical hepatic dysfunction after laparoscopic cholecystectomy (LC) has been described in the literature. However, this alteration is not encountered in all patients. In order to address this situation, a prospective study was conducted to investigate the effect of abdominal perfusion pressure (APP) on liver function tests after LC performed under constant intra-abdominal pressure (IAP). PATIENTS AND METHODS Of 78 patients who underwent LC between May 2007 and October 2007, 40 patients were eligible for the study. In all the patients, six parameters of liver function (aspartate aminotransferase, alanine aminotransferase, direct bilirubin, indirect bilirubin, alkaline phosphatase, and gamma-glutamyltransferase) were assessed before and 24 hours after surgery. Patients who showed more than a 100% increase in at least one parameter (group 1) were compared to those who did not (group 2) regarding age, sex, body weight, body height, operation time, pneumoperitoneum time, IAP, preoperative, and intraoperative APP. RESULTS Of the patients, 37.5% showed more than a 100% increase in at least one parameter of liver function. No significant difference was found between the two groups with regard to age, sex, body weight, body height, operation time, pneumoperitoneum time, and IAP. There were significant increase in AST and ALT at 24 hours postoperatively in group 1, as compared with group 2 (P = 0.000, P = 0.001). In comparison of preoperative APP with intraoperative APP values, group 1 showed a statistically significant decrease (P = 0.000), while no difference was found in group 2. CONCLUSION Subclinical hepatic dysfunction after LC could mostly be attributed to the negative effects of the pneumoperitoneum on hepatic blood flow. For the evaluation of hepatic hypoperfusion, APP may be a new criterion as a determinant of interaction with mean arterial pressure (MAP) and IAP.

Tumor deposits in gastric carcinomas

We performed this study to examine the prevalence of tumor deposits (TD) in gastric adenocarcinomas (GACa), and the relevance of their presence, size and type to clinical outcome. Ninety-six patients, histopathologically diagnosed as GACa following a total/subtotal gastrectomy were included, and clinicopathologic data were recorded. Due to the statistical analysis, the majority of TD(+) cases were of intestinal type and showed vascular invasion. In these cases, the incidence of local recurrence was significantly higher. The majority of GACa of intestinal type with TD were of high grade and showed vascular invasion. Recurrence and death were more commonly encountered among them. The recurrence-free survival (RFS) was significantly shorter in patients with TDs, which was also confirmed by multivariate analysis, and there was a significant difference between both RFS and overall survival of TD(+) and TD(-) cases of intestinal type GACa. In conclusion, in this study, we demonstrate that TDs are not infrequently observed in GACa, they are more commonly associated with the intestinal type and vascular invasive gastric cancers. Our study shows the prognostic impact of TDs, especially regarding the RFS. Therefore, the documentation of TDs might be considered for prospective studies, especially for the intestinal type GACa, a shortcoming of this study.

Combined Liver-Kidney Transplantation and Follow-Up in Primary Hyperoxaluria Treatment: Report of Three Cases

INTRODUCTION Primary hyperoxaluria type-1 (PH1) is an autosomal recessive disorder caused by impaired activity of the hepatic peroxisomal alanine-glyoxilate aminotransferase, which leads to end-stage renal disease (ESRD) and requires combined liver-kidney transplantation (CLKT). Herein, we have reported 3 children diagnosed with PH1 who received CLKT. CASE 1: A 4.5-year-old boy with an elder brother diagnosed with PH1 was diagnosed during family screening when the sonography showed multiple calculi. Within 5 years he experienced flank pain, hematuria attacks, and anuric phases due to obstruction and received hemodialysis (HD) when ESRD appeared. CLKT was performed from his full-match sister at the age of 9.5. He is doing well at 5.5 years. CASE 2: A 7-year-old boy was admitted with polyuria, polydypsia, and stomach pain with renal stones on sonography. PD was instituted when serum creatinine and BUN levels were measured as high values. At the age of 10, CKLT was performed from his mother. His liver and renal function tests are well at 14 months after CKLT. CASE 3: A 2.5-year-old girl had attacks of dark urine without any pain; renal stones were imaged on sonography. She was diagnosed with PH1 and operated on several times due to obstruction. She received peritoneal dialysis and a cadaveric CLKT was performed when she was 9 years old. At the age of 16, she experienced chronic allograft nephropathy requiring HD and subsequent cadaveric donor renal transplantation at 1.5 years after initiation of HD. CONCLUSION Herein, we have presented the favorable clinical outcomes of patients with CKLT to indicate the validity of this treatment choice for PH1.

Successful Therapy With Rituximab of Refractory Acute Humoral Renal Transplant Rejection: A Case Report

Acute humoral rejection (AHR) is generally less responsive to conventional anti-rejection treatment with consequent allograft losses. Therapeutic options include antilymphocyte antibody (ATG), intravenous immunglobulin (IVIG), plasmapheresis, or immunoadsorption with protein A together with intensification of immunsuppression with a tacrolimus/mycophenolate mofetil combination. This report describes a transplant recipient who responded to rituximab therapy as treatment for steroid-, ATG-, IVIG-, and plasmapheresis-resistant AHR.

A case of osteopoikilosis coexisting with amyloidosis of familial Mediterranean fever

Osteopoikilosis is a rare benign sclerosing bone dysplasia that is asymptomatic and often diagnosed incidentally. Multiple small, round or oval sclerotic foci are found, particularly in the pelvis and epiphyses and metaphyses of the long bones. It has an autosomal dominant mode of inheritance [1]. It is commonly associated with small subcutaneous fibrous nodules called dermatofibrosis lenticularis disseminate (Buschke-Ollendorff syndrome) [1, 2]. However, patients with osteopoikilosis and rheumatoid arthritis, polyarthralgia, synovial chondromatosis, dacriocystitis, trisomy 8, connective tissue disorders affecting the skin and joints, and discoid lupus erythematosus have also been reported [3, 4, 5, 6, 7, 8, 9]. Here we report a pediatric renal transplant recipient with amyloidosis secondary to familial Mediterranean fever (FMF) who was incidentally diagnosed as having osteopoikilosis in the 6th post-transplant year. To our knowledge, this is the first reported case of osteopoikilosis diagnosed in a child with amyloidosis associated with FMF. A 10-year-old boy with end-stage renal disease due to amyloidosis associated with FMF received a kidney graft from his father. He was given triple immunosuppressive therapy with prednisone, azathioprine, and cyclosporin A. Colchicine treatment was also continued. He has been followed for 84 months with this treatment. In the 6th post-transplant year, a plain chest X-ray obtained during routine screening for tuberculosis revealed small radiodense lesions in the right 3rd and 5th ribs and in left 4th and 5th ribs (Fig. 1). Pelvic X-ray (Fig. 2) showed similar lesions in the pelvic bones. He was asymptomatic, and the diagnosis was consistent with osteopoikilosis. Family screening for this disease was negative. Since osteopoikilosis has been reported to have an autosomal dominant form of inheritance, absence of osteopoikilosis in other family members may be related to incomplete penetrance and/or spontaneous mutation in the