Sf Gottlieb

Reduction of rattlesnake-venom-induced myonecrosis in mice by hyperbaric oxygen therapy

Hyperbaric oxygen therapy (HBOT) at 1, 2, and 2.75 atmospheres absolute (ATA) was used to treat rattlesnake (Crotalus atrox) venom-induced tissue damage and edema in thigh muscles of mice. Tissue damage was evaluated by double-blind histopathologic examination: tissue edema was determined by measuring tissue water content. A total of 10 intermittent exposures to oxygen over a period of 4 days at 2 and 2.75 ATA did not influence the resolution of venom-induced tissue edema, whereas tissue damage was significantly ameliorated as compared to air-treated envenomated controls. HBOT also promoted healing in the venom-injected mice as evidenced by the presence of regenerating muscle cells. It is concluded that HBOT may limit rattlesnake venom-induced myonecrosis and promote healing in a dose-response relationship without reducing venom-induced edema.

Enhancement of cortical Na+,K+-ATPase by increased oxygen tensions: evidence of a new controlling mechanism.

This study describes the preliminary isolation of substances from beef brain cortex which are required to produce an oxygen-induced enhancing effect on Na, K-ATPase. Evidence is presented that at least 3 fractions--a heat stable, low molecular weight proteinaceous substance, a cholesterol rich, membranous component, and an as yet unidentified substance--are required to produce oxygen enhancement of Na, K-ATPase activity. These findings have specific ramifications in neurocellular physiology, especially as related to seizures.

Application of Fourier filtering techniques for determining redox change in cerebral cortical cytochrome oxidase

The use of near infrared spectroscopy for measuring cellular oxygen availability with cytochrome oxidase redox measurements was proposed in 1977. However, accuracy of measurement in blood-perfused tissue has been controversial because of the difficulty of separating the hemoglobin cytochrome oxidase spectra. This paper proposes the use of spectra wavelength intervals recorded by a cooled charge coupled device type spectrophotometer and fast Fourier transfer filtering techniques for aa3 signal extraction. Results indicate the proposed technique and equipment are excellent candidates for non-invasive monitoring oxygen availability to cells in vivo.

Cytochrome oxidase reduction/oxidation charge-coupled monitor with large-area pickup optode

Except for amplitude, the near IR spectra recorded through intact scalp and skull were identical to those from brain surface recordings. Reduction of scattering due to hypoxia was clearly evident in nitrogen induced spectra. Absorbance peaks, hemoglobin and aa3, were visible and did not seem to be affected by percentage of deoxyhemoglobin. The 600 nm peak is believed to be caused by scattering, not aa3 absorbance. Work is continuing to further demonstrate the validity of the hemoglobin and aa3 absorption peaks.