Sha Jingjie

Effect of nanopore size on poly(dT) 30 translocation through silicon nitride membrane

The nanopore size effect on translocation of poly(dT)30 through Si3N4 membrane is investigated. In this paper, we report that the speed of the poly(dT)30 transport through Si3N4 nanopores can be slowed down by half through increasing the nanopore diameter from 4.8 nm to 10.8 nm. The results are consistent with our simulation results. Besides, the current blockage induced by DNA passing through the nanopore is less obvious as pore diameter is larger, which is in good agreement with the theoretical prediction. The conclusion about DNA transport through nanopores is beneficial for the design of DNA sequencing devices.

Integrated solid-state nanopore devices for third generation DNA sequencing

Third generation DNA sequencing relies on monitoring the ionic current blockage during the DNA molecule’s threading through a nanoscale pore. It is still really tough to attain the single base discrimination on a DNA strand by merely analyzing the ionic current due to speedy DNA translocation and low spatial resolution. More integrated configurations are pursued to present versatile comparative dissimilarities of the four bases by enhancing the spatial resolution within a DNA molecule translocation event, such as transverse tunneling current, local potential change, and capacitance oriented voltage resonance. In this mini review, the insight is provided into the status quo on several functionalized techniques and methodologies for DNA sequencing and furthermore concluding remark and outlook are presented.