Shahi Imam Reja

A Charge Transfer Amplified Fluorescent Hg2+ Complex for Detection of Picric Acid and Construction of Logic Functions

A chemosensor 3 based on the N,N-dimethylaminocinnamaldehyde has been synthesized which shows fluorescence turn-on response with Hg(2+) ions, and the in situ prepared 3-Hg(2+) complex has been used for detection of picric acid via electrostatic interaction and construction of a combinatorial logic circuit with NOR and INHIBIT logic functions.

d-PET coupled ESIPT phenomenon for fluorescent turn-on detection of hydrogen sulfide

A d-PET coupled ESIPT based probe has been designed and synthesized for the selective detection of H2S, among the other ions and various sulfur species such as cysteine and glutathione, in aqueous media via the hydrogen sulfide induced reduction of nitro functionalities, and has further been utilized for the imaging of H2S in intracellular systems.

An Approach for the Selective Detection of Nitric Oxide in Biological Systems: An in vitro and in vivo Perspective.

A naphthalimide-based fluorescent probe, LyNP-NO, was designed and synthesized for the selective detection of exogenously and endogenously generated nitric oxide (NO) in C6 glial cells. In addition, LyNP-NO was also explored for monitoring endogenous NO levels in rat hippocampus at various tissue depths by stimulating the brain with N-methyl-d-aspartate (NMDA).

A bodipy based fluorescent probe for evaluating and identifying cancer, normal and apoptotic C6 cells on the basis of changes in intracellular viscosity

The applications of a bodipy based probe 1 for the identification of diseased cell population out of normal cells on the basis of changes in intracellular viscosity have been explored. Probe 1 works on the principle of restriction of rotation in viscous medium and the molecular rotor nature of probe 1 is supported by low temperature 1H NMR and variable dihedral angle DFT and TD-DFT studies. More importantly, probe 1 is the first probe which shows its practical application in monitoring micro-viscosity changes in a cell based model system of undifferentiated, differentiated and apoptotic C6 glial cells. Further, probe 1 can effectively monitor the apoptosis pathway by showing an increase in fluorescence intensity from cancerous cells to apoptotic cells via real time live-cell video imaging. Moreover, the viscosity changes in living cells were proved by fluorescence lifetime imaging (FLIM) studies, flow cytometry using Annexin-V and Bcl-xl expression by immunocytofluorescence (ICC) and western blot analysis.

Chemodosimeter approach for nanomolar detection of Cu2+ ions and their bio-imaging in PC3 cell lines

A new rhodamine–azaindole based receptor 4 has been synthesized which selectively senses Cu2+ ions via a fluorescence resonance energy transfer (FRET) process in acetonitrile while in mixed aqueous media it undergoes Cu2+ promoted hydrolysis among the various metal ions tested (Pb2+, Ba2+, Fe3+, Cd2+, Ag+, Zn2+, Hg2+, Cu2+, Ni2+, Co2+, Fe2+ and Mg2+). The time resolved fluorescence studies also support the Cu2+ promoted hydrolysis of receptor 4 in mixed aqueous media. The detection limit for Cu2+ ions has been found to be 20 nM. The potential biological application of probe 4 is evaluated for in vitro detection of copper ions in prostate cancer (PC3) cell lines.

A Highly Selective Fluorescent Probe for Detection of Hydrogen Sulfide in Living Systems: in vitro and in vivo Applications

A fluorescein-based fluorescent probe has been designed and synthesised that selectively detects H2 S in aqueous medium, among various analytes tested. This fluorescein-based fluorescent probe has also been successfully utilised for real-time imaging of exo- and endogenously produced H2 S in cancer cells and normal cells. Moreover, the probe can also detect H2 S in the rat brain hippocampus at variable depths and in living nematodes.