Shahid H. Ansari

Volatile Constituents of the Fruits of Three Mango Cultivars, Mangifera indica L.

Abstract The chemical composition of the fruit oils are reported for Mangifera indica L., cultivar ‘Langra,’ cultivar ‘Bombay’ and cultivar ‘Desi’ from India. The oils were obtained by hydrodistillation and analyzed by GC and GC/MS. The oil of cultivar ‘Langra’ was characterized by δ-3-carene (61.7%) as major constituent. The oil of cultivar ‘Bombay’ was found to contain α-pinene (26.6%), caryophyllene oxide (14.5%) and humulene oxide (5.3%). The oil of cultivar ‘Desi’ had considerable p-cymen-8-ol (28.6%) and moderate amounts of δ-3-carene (5.7%).

New 4′-substituted flavones from the fruit peels of Citrus limon (L.) Burm.f.

Two new 4′-substituted flavones isolated from the fruit peels of Citrus limon (L.) Burm.f. (Rutaceae) have been characterized as 4′-(9′-ethylene-16′-methylnon-9′,15′-dien-7′,11′-oate)-5,7-dimethoxyflavone (limonflavonyl lactone A, 1) and 4′-(9′-ethylene-16′-methylnon-9′,15′-dien-7′,11′-oate)-5,7,3′-trimethoxyflavone (limonflavonyl lactone B, 2) on the basis of spectral data and chemical analyses. Both the flavones are reported for the first time from a plant source.

HPLC and HPTLC Methods by Design for Quantitative Characterization and in vitro Anti-oxidant Activity of Polyherbal Formulation Containing Rheum emodi

Safoof-e-Pathar phori (SPP) is a traditional polyherbal formulation and has been used since long time for its anti-urolithiatic activity. It contains three plant constituents Didymocarpous pedicellata, Dolichous biflorus and Rheum emodi. Emodin and chrysophanic acid were selected as chemical markers for SPP and quantified using simultaneous HPTLC and RP-HPLC methods in R. emodi and in SPP. The simultaneous methods were found linear r(2) = 0.991 in a wide range (10-800 ng spot(-1) with HPTLC, 5-500 µg mL(-1) with HPLC) precise, accurate and robust for both the drugs. Anti-oxidant activity of SPP, R. emodi as well as standard emodin and chrysophanic acid were determined by using DPPH (2,2-diphenyl-1-picryl hydrazyl radical), which showed better activity of R. emodi (IC50 = 12.27) extract when compared with SPP (IC50 = 32.99) and standard drugs (IC50 = 66.81). The robustness of methods were proved by applying the Box-Behnken response surface design software and other validation parameters evaluated were satisfactorily met; hence, the developed method found suitable for application in the quality control of several formulations containing emodin and chrysophanic acid.

Volatile Constituents of the Galls and Stem Bark of Pistacia integerrima Stew. Ex Brandis

Abstract Steam distilled oil from the galls and stem bark of Pistacia integerrima Stew. ex Brandis, grown in India, were analyzed by capillary GC and GC/MS. The gall volatiles contained 38 components of which 91% were found to be monoterpenes. The bark oil was found to contain 22 constituents of which 82.3% were monoterpenes. The major constituents of the gall oil were α-pinene (34.5%), sabinene (14.0%), β-pinene (12.6%), and limonene (9.1%). The important components of the bark oil were terpinen-4-ol (30.3%), α-terpineol (13.7%) and α-pinene (14.0%).