Shammi Kapoor

Optimised isolation of polysaccharides from Lentinula edodes strain NCBI JX915793 using response surface methodology and their antibacterial activities

Mycelial growth in a defined medium by submerged fermentation is a rapid and alternative method for obtaining fungal biomass of consistent quality. Biomass, exopolysaccharides (EPS) and intracellular polysaccharides (IPS) production were optimised by response surface methodology in Lentinula edodes strain LeS (NCBI JX915793). The optimised conditions were pH 5.0, temperature 26°C, incubation period of 25 days and agitation rate of 52 r/min for L. edodes strain LeS. Under the calculated optimal culture conditions, biomass production (5.88 mg mL− 1), EPS production (0.40 mg mL− 1) and IPS production (12.45 mg g− 1) were in agreement with the predicted values for biomass (5.93 mg mL− 1), EPS (0.55 mg mL− 1) and IPS production (12.64 mg g− 1). Crude lentinan exhibited highest antibacterial effects followed by alcoholic, crude and aqueous extracts. The results obtained may be useful for highly effective yield of biomass and bioactive metabolites.

Region based Diversity Studies of Saccharomyces cerevisiae and Lentinula edodes

India is a rich repository of microbial genetic diversity. There are number of fungal species that are associated with the eating habits and health benefits of the Indians. The most famous yeast comes under ascomycetes family of fungi, Saccharomyces cerevisiae, which is associated with most of the fermented foods of the Indian origin (Walia et al., 2014). The fermented foods are more common among people belonging to the rural areas, especially in hilly and tribal people. The quality of a fermented product is consequence of the starter and progressive presence of microbial flora during the process of fermentation. To obtain the best strain, the knowledge of natural yeast diversity available in the given area or yeast(s) associated with a particular product, is of prime importance (Kanwar and Keshani 2014). Eighteen S. cerevisiae strains isolated from various fermented foods of Western International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 07 (2018) Journal homepage: http://www.ijcmas.com