Sharad Medhe

Antioxidant activity of essential oil of Coriandrum sativum and standardization of HPTLC method for the estimation of major phytomarkers

The essential oils of seed and whole plant of Coriandrum sativum were examined for their in vitro antioxidant activities which were found to be higher compared to different extracts of this plant. A simple, precise and specific HPTLC method has been developed for the determination of important phytomarkers of essential oils. The proposed method was validated in terms of linearity, precision, accuracy, limits of detection and quantification.

Antioxidant and Antimutagenic Activities of Isothiocyanates Rich Seed Oil of Eruca sativa Plant

Eruca sativa seed oil has been explored for antioxidative and antimutagenic potential. Seed oil exhibited maximum percentage inhibition of hydroxyl (93.42 %) and 2,2-diphenyl-1-picrylhydrazyl (92.02 %) radicals at the concentration of 90 μg/ml. Treatment of mice with seed oil significantly (p<0.01) reduced oxidative stress and attenuated the altered changes of reduced glutathione. Seed oil treated group reduced the aberrant metaphase (47.50 %) and micronucleus formation (85 %) in the bone marrow of melanoma induced mice. The presence of isothiocyanates [allyl (40.3 μg/g), 3-butenyl (259.6 μg/g), 4-methylsulfinybutyl (743.1 μg/g), 2-phenylethyl (158.5 μg/g) and bis(isothiocyanatobutyl)disulphide (~5000 μg/g)] was confirmed by HS/SPME/GC-MS analysis of seed oil and found to be responsible for the observed bioefficacy. Our findings support the use of E. sativa seed oil (ignored edible oil) as health promoting food and its potential for clinical use.

High Performance Thin Layer Chromatographic Method for the Estimation of Cholesterol in Edible Oils

Cholesterol was detected in six edible market available oil brands using high performance thin layer chromatography. Standard conditions have been optimized based on simulation in Rf values under experimental conditions of polarity of mobile phase and saturation time of solvent chamber. The peanut oil contains highest (0.71 %) while coconut oil contains lowest (0.15 %) cholesterol level. Among the oils studied, no oil was found cholesterol free.

Antimelanoma potential of eruca sativa seed oil and its bioactive principles

The present communication reports the comparison of in vivo antioxidant, antimelanoma and antimutagenic activities of Eruca sativa seed oil and its bio principles (allyl isothiocyanate, phenylethyl isothiocyanate and sulphoraphane) against B16F10 melanoma cells induced in C57BL/6 mice model. Among the various treatments considered for the study, isothiocyanates combination (allyl isothiocyanate, phenylethyl isothiocyanate and sulphoraphane; 1:1:1; 10 µM) exhibited optimum antioxidant activity, 51.95±1.14 µM glutathione per mg protein compared to seed oil 25.91±1.26 µM. Lipid peroxidation value was 9.97±1.72 µM malondialdehyde per mg wet weight for isothiocyanates combination against seed oil, 28.45±1.87 µM and rendered significant protection against oxidative stress induced by melanoma in liver tissue. Isothiocyanates combination significantly suppressed various parameters, such as tumor growth, isothiocyanates combination by 36.36% while the seed oil by 15.23%; tumor weight, isothiocyanates combination by 45.9% and seed oil by 19.6%; tumor volume, isothiocyanates combination by 41.7% while the seed oil by 32.3%, measured for antimelanoma activity at a concentration of 10 µM. Isothiocyanates combination has been found to be more cytotoxic bioagent against B16F10 melanoma cells induced in C57BL/6 mice compared to naturally occurring Eruca sativa seed oil.