Shengsong Huang
Tongji University
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Publication
Featured researches published by Shengsong Huang.
Journal of Experimental & Clinical Cancer Research | 2015
Jun Xiang; Cuidong Bian; Hao Wang; Shengsong Huang; Denglong Wu
ObjectiveEvidence supports an important role for miR-203 in the regulation of the proliferation, migration and invasion of prostate cancer (PCa) cells. However, the exact mechanisms of miR-203 in PCa are not entirely clear.MethodsWe examined the expression of miR-203 in prostate cancer tissues, adjacent normal tissues, PCa cell lines and normal prostate epithelial cells by qRT-PCR. Then, the effects of miR-203 or Rap1A on proliferation, adhesion and invasion of PCa cells were assayed using CKK-8, adhesion analysis, and transwell invasion assays. Luciferase reporter assay was performed to assess miR-203 binding to Rap1A mRNA. Tumor growth was assessed by subcutaneous inoculation of cells into BALB/c nude mice.ResultsHere, we confirmed that the expression of miR-203 was significantly downregulated in prostate cancer specimens compared with matched adjacent normal prostate specimens. Mechanistic dissection revealed that miR-203 mediated cell proliferation, adhesion and invasion in vitro, and tumor growth in vivo, as evidenced by reduced RAC1, p-PAK1, and p-MEK1 expression. In addition, we identified Rap1A as a direct target suppressed by miR-203, and there was an inverse relationship between the expression of miR-203 and Rap1A in PCa. Knockdown of Rap1A phenocopied the effects of miR-203 on PCa cell growth and invasion. Furthermore, Rap1A over-expression in PCa cells partially reversed the effects of miR-203-expression on cell adhesion and invasion.ConclusionsThese findings provide further evidence that a crucial role for miR-203 in inhibiting metastasis of PCa through the suppression of Rap1A expression.
Urologic Oncology-seminars and Original Investigations | 2013
Tao Li; Shengsong Huang; Minghua Dong; Yaping Gui; Denglong Wu
OBJECTIVES To investigate small ubiquitin-like modifier (SUMO)-specific protease 1 (SENP1) expression in human prostate cancer (CaP) cells and its prognostic value for CaP patients after radical prostatectomy (RP). MATERIALS AND METHODS SENP1 expression in CaP cells was detected by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) and Western blotting. By using immunohistochemistry coupled with the tissue microarray (TMA) technique, we examined SENP1 protein expression in 115 specimens of CaP, 19 prostatic intra-epithelial neoplasia (PIN) tissues, and 24 normal prostate tissues. Moreover, correlations between SENP1 protein expression, clinicopathologic features, and prognosis were statistically analyzed. RESULTS Three CaP cells, DU145, PC-3, and LNCaP had overexpression of SENP1 mRNA and protein, while the nontransformed immortalized prostate cell RWPE-1 had relatively weak SENP1 expression. Especially, DU145, a hormone-independent CaP cell line, showed higher transcriptional and translational level of SENP1 than the others. SENP1 protein expression correlated with some clinicopathologic parameters, such as pathologic stage, Gleason score, and biochemical recurrence (BCR). Positive SENP1 immunostaining in the CaP, PIN, and normal prostate tissue samples were 76.5%, 57.9%, and 4.2%, respectively. CaP patients undergoing RP with positive SENP1 expression were significantly associated with poor biochemical-free survival. Multivariate analysis indicated that SENP1 protein expression was an independent prognostic factor for BCR-free survival after RP. CONCLUSIONS This study confirmed the up-regulation of SENP1 mRNA and protein level in CaP cells. We suggested that SENP1 expression might contribute to the malignant progression of CaP. Importantly, SENP1 presented as a potential prognostic factor for BCR after RP.
BJUI | 2012
Tao Li; Yaping Gui; Tao Yuan; Guoqiang Liao; Cuidong Bian; Qiquan Jiang; Shengsong Huang; Bo Liu; Denglong Wu
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International Journal of Clinical and Experimental Medicine | 2014
Bo Liu; Lei Wang; Shengsong Huang; Qiang Wu; Deng-Long Wu
American Journal of Translational Research | 2015
Xin Zhao; Wei He; Junliang Li; Shengsong Huang; Xiaodong Wan; Huarong Luo; Denglong Wu
International Journal of Clinical and Experimental Medicine | 2015
Junliang Li; Xuechao Wan; Wu Qiang; Tao Li; Wenhua Huang; Shengsong Huang; Denglong Wu; Yao Li
American Journal of Translational Research | 2015
Xin Zhao; Junliang Li; Shengsong Huang; Xiaodong Wan; Huarong Luo; Denglong Wu
International Journal of Clinical and Experimental Medicine | 2014
Xin Zhao; Shengsong Huang; Huarong Luo; Xiaodong Wan; Yaping Gui; Junliang Li; Denglong Wu
Lasers in Medical Science | 2016
Gang Wu; Zhe Hong; Chao Li; Cuidong Bian; Shengsong Huang; Denglong Wu
International Journal of Clinical and Experimental Medicine | 2014
Wei Le; Shengsong Huang; Yaping Gui; Huarong Luo; Denglong Wu; Huailiang Feng; Jinfu Zhang