Shi Jisen

Interaction Characteristics of the Co-incubation between CdSe/ZnS Quantum Dots and Liriodendron Hybrids Suspension Cells by PEG-mediation

Recently, there is a growing concern on targeting medical treatments or animal transgenic research by nanoparticles-mediation. However, transportation of molecules into plant cells is more complicated due to the plant cell wall––an extra barrier in addition to the cell membrane. We envisaged the surface modification of nanoparticles would improve the uptake of intact plant cells. Here, by regulating the surface charge of CdSe/ZnS quantum dots (QDs), incubation time and temperature, the interactions between CdSe/ZnS QDs and the walled plant cells have been investigated. The results show that dependent on incubation time and temperature, CdSe/ZnS QDs with positive charges have the capability to penetrate the cell wall and cell membrane of intact plant cells by fluid-phase endocytosis, and did not inhibit cell bioactivity. However, CdSe/ZnS QDs with negative charges mainly clustered near the cell walls. In the case of polyethylene glycol (PEG)-mediation, the endocytic capability of intact plant cells could be significantly enhanced and they release their cytotoxicity. Therefore, CdSe/ZnS QDs with positive charges can be widely applied as the transgenic nano-carriers for plant suspension cells.

Identification of seeds of Pinus species by Microsatellite Markers

The 276 pair-primers (nuclear and chloroplast microsatellite) developed from seven species of Pinaceae were selected and identified for cross-species transferability to tenPinus species (P. massoniana, P. kesiya, P. tabulaeformis, P. densiflora, P. thunbergii, P. caribaea, P. taeda, P. yunnanensis, P. densata, P. sylvestris) belonging to Sect.Pinus by BSA (bulked segregate analysis) method. The results showed that 23 of 276 (8.0%) markers were successful to have amplification product in ten species, and 5 of 23 (21.7%) were polymorphic cross species and lack of polymorphic within species. Eight of 10Pinus species were identified by using single primer, two and more combination of primers, but there were still no effective SSR primers for identifying other 2 species (P. kesiya andP. densata).