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Dive into the research topics where Shichang Zhao is active.

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Featured researches published by Shichang Zhao.


Journal of Materials Chemistry B | 2014

3D-printed magnetic Fe3O4/MBG/PCL composite scaffolds with multifunctionality of bone regeneration, local anticancer drug delivery and hyperthermia

Jianhua Zhang; Shichang Zhao; Min Zhu; Yufang Zhu; Yadong Zhang; Zhongtang Liu; Changqing Zhang

In this study, three-dimensional (3D) magnetic Fe3O4 nanoparticles containing mesoporous bioactive glass/polycaprolactone (Fe3O4/MBG/PCL) composite scaffolds have been fabricated by the 3D-printing technique. The physiochemical properties, in vitro bioactivity, anticancer drug delivery, mechanical strength, magnetic heating ability and cell response of Fe3O4/MBG/PCL scaffolds were systematically investigated. The results showed that Fe3O4/MBG/PCL scaffolds had uniform macropores of 400 μm, high porosity of 60% and excellent compressive strength of 13-16 MPa. The incorporation of magnetic Fe3O4 nanoparticles into MBG/PCL scaffolds did not influence their apatite mineralization ability but endowed excellent magnetic heating ability and significantly stimulated proliferation, alkaline phosphatase (ALP) activity, osteogenesis-related gene expression (RUNX2, OCN, BSP, BMP-2 and Col-1) and extra-cellular matrix (ECM) mineralization of human bone marrow-derived mesenchymal stem cells (h-BMSCs). Moreover, using doxorubicin (DOX) as a model anticancer drug, Fe3O4/MBG/PCL scaffolds exhibited a sustained drug release for use in local drug delivery therapy. Therefore, the 3D-printed Fe3O4/MBG/PCL scaffolds showed the potential multifunctionality of enhanced osteogenic activity, local anticancer drug delivery and magnetic hyperthermia.


Acta Biomaterialia | 2015

Three-dimensional printed strontium-containing mesoporous bioactive glass scaffolds for repairing rat critical-sized calvarial defects.

Shichang Zhao; Jianhua Zhang; Min Zhu; Yadong Zhang; Zhongtang Liu; Cuilian Tao; Yufang Zhu; Changqing Zhang

The development of a new generation of biomaterials with high osteogenic ability for fast osseointegration with host bone is being intensively investigated. In this study, we have fabricated three-dimensional (3-D) strontium-containing mesoporous bioactive glass (Sr-MBG) scaffolds by a 3-D printing technique. Sr-MBG scaffolds showed uniform interconnected macropores (∼400μm), high porosity (∼70%) and enhanced compressive strength (8.67±1.74MPa). Using MBG scaffolds as a control, the biological properties of Sr-MBG scaffolds were evaluated by apatite-forming ability, adhesion, proliferation, alkaline phosphatase activity and osteogenic gene expression of osteoblast-like cells MC3T3-E1. Furthermore, Sr-MBG scaffolds were used to repair critical-sized rat calvarial defects. The results showed that Sr-MBG scaffolds possessed good apatite-forming ability and stimulated MC3T3-E1 cell proliferation and differentiation. Importantly, the in vivo results revealed that Sr-MBG scaffolds had good osteogenic capability and stimulated new blood vessel formation in critical-sized rat calvarial defects within 8 weeks. Therefore, 3-D printed Sr-MBG scaffolds with favorable pore structure and high osteogenic ability have more potential applications in bone regeneration.


Journal of Materials Chemistry B | 2014

Evaluation of borate bioactive glass scaffolds as a controlled delivery system for copper ions in stimulating osteogenesis and angiogenesis in bone healing

Hui Wang; Shichang Zhao; Jie Zhou; Youqu Shen; Wenhai Huang; Changqing Zhang; Mohamed N. Rahaman; Deping Wang

Biocompatible synthetic scaffolds with enhanced osteogenic and angiogenic capacity are of great interest for the repair of large (critical size) bone defects. In this study, we investigated an approach based on the controlled delivery of copper (Cu) ions from borate bioactive glass scaffolds for stimulating angiogenesis and osteogenesis in a rodent calvarial defect model. Borate glass scaffolds (pore size = 200-400 μm) doped with varying amounts of Cu (0-3.0 wt% CuO) were created using a polymer foam replication technique. When immersed in simulated body fluid (SBF) in vitro, the scaffolds released Cu ions into the medium at a rate that was dependent on the amount of Cu in the glass and simultaneously converted to hydroxyapatite (HA). At the concentrations used, the Cu in the glass was not cytotoxic to human bone marrow derived stem cells (hBMSCs) cultured on the scaffolds and the alkaline phosphatase activity of the hBMSCs increased with increasing Cu in the glass. When implanted in rat calvarial defects for 8 weeks, the scaffolds doped with 3 wt% CuO showed a significantly better capacity to stimulate angiogenesis and regenerate bone when compared to the undoped glass scaffolds. Together, these results indicate that the controlled delivery of Cu ions from borate bioactive glass implants is a promising approach in healing bone defects.


Journal of Materials Chemistry B | 2014

Three dimensionally printed mesoporous bioactive glass and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) composite scaffolds for bone regeneration

Shichang Zhao; Min Zhu; Jianhua Zhang; Yadong Zhang; Zhongtang Liu; Yufang Zhu; Changqing Zhang

Development of bioactive scaffolds with controllable architecture and high osteogenic capability for bone tissue engineering is hotly pursued. In this study, three-dimensional (3D) mesoporous bioactive glass (MBG) and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) composite scaffolds with well-defined pore structures and high compressive strength (∼5-12 MPa) were synthesized by a 3D printing technique. Compared to reported polymer-bonded MBG scaffolds, the incorporation of the biocompatible PHBHHx polymer as a particle binder enhanced their bioactive and osteogenic properties, including fast apatite-forming ability, and promoted human bone marrow-derived mesenchymal stem cell (hBMSC) adhesion, proliferation, alkaline phosphatase (ALP) activity and bone-related gene expression. Furthermore, MBG/PHBHHx composite scaffolds were explored to repair critical-size rat calvarial defects. The results showed that MBG/PHBHHx composite scaffolds exhibited a controlled degradation rate and more significant potential to stabilize the pH environment with increasing PHBHHx ratio. At 8 weeks post-implantation, MBG/PHBHHx scaffolds were demonstrated to stimulate bone regeneration in the calvarial defects and have largely repaired them through analysis of micro-CT, sequential fluorescent labeling and histology. These results lay a potential framework for future study by using modified MBG/PHBHHx-based functional scaffolds to improve the osteogenic activity and bone defect restoration.


Acta Biomaterialia | 2015

RETRACTED: Copper-doped borosilicate bioactive glass scaffolds with improved angiogenic and osteogenic capacity for repairing osseous defects

Shichang Zhao; Hui Wang; Yadong Zhang; Wenhai Huang; Mohamed N. Rahaman; Zhongtang Liu; Deping Wang; Changqing Zhang

There is growing interest in the use of synthetic biomaterials to deliver inorganic ions that are known to stimulate angiogenesis and osteogenesis in vivo. In the present study, we investigated the effects of varying amounts of copper in a bioactive glass on the response of human bone marrow-derived mesenchymal stem cells (hBMSCs) in vitro and on blood vessel formation and bone regeneration in rat calvarial defects in vivo. Porous scaffolds of a borosilicate bioactive glass (composition 6Na2O, 8K2O, 8MgO, 22CaO, 36B2O3, 18SiO2, 2P2O5, mol.%) doped with 0.5, 1.0 and 3.0wt.% CuO were created using a foam replication method. When immersed in simulated body fluid, the scaffolds released Cu ions into the medium and converted to hydroxyapatite. At the concentrations used, the Cu in the glass was not toxic to the hBMSCs cultured on the scaffolds in vitro. The alkaline phosphatase activity of the hBMSCs and the expression levels of angiogenic-related genes (vascular endothelial growth factor and basic fibroblast growth factor) and osteogenic-related genes (runt-related transcription factor 2, bone morphogenetic protein-2 and osteopontin) increased significantly with increasing amount of Cu in the glass. When implanted in rat calvarial defects in vivo, the scaffolds (3wt.% CuO) significantly enhanced both blood vessel formation and bone regeneration in the defects at 8weeks post-implantation. These results show that doping bioactive glass implants with Cu is a promising approach for enhancing angiogenesis and osteogenesis in the healing of osseous defects.


Journal of Materials Chemistry B | 2015

Effects of functional groups on the structure, physicochemical and biological properties of mesoporous bioactive glass scaffolds

Shichang Zhao; Jianhua Zhang; Min Zhu; Yadong Zhang; Zhongtang Liu; Yanyu Ma; Yufang Zhu; Changqing Zhang

Functionalization of biomaterials with specific functional groups is one of the most straightforward strategies to induce specific cell responses to biomaterials. In this study, thiol (SH) and amino (NH2) functional groups have been successfully modified on the surfaces of mesoporous bioactive glass (MBG) scaffolds to form thiol-functionalized MBG (SH-MBG) and amino-functionalized MBG (NH2-MBG) scaffolds by a post-grafting technique. The effects of the functional groups on the structure, physicochemical and biological properties of MBG scaffolds were systematically investigated. The results showed that the functionalization of MBG scaffolds did not change their structures, and the SH-MBG and NH2-MBG scaffolds still had hierarchical pore architecture (macropores of 300-500 μm and mesopores of 3.5-4 nm) and high porosity (84-86%), similar to the MBG scaffolds. Furthermore, the SH-MBG and NH2-MBG scaffolds possessed similar apatite mineralization ability and biocompatibility compared to the MBG scaffolds. Importantly, the SH-MBG and NH2-MBG scaffolds significantly stimulated adhesion, proliferation and differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs). Therefore, functionalization of MBG scaffolds with SH and NH2 functional groups would be a viable way to tailor the surface characteristics for stimulating biological responses of hBMSCs, and the functionalized MBG scaffolds would be a promising bioactive material for bone tissue engineering applications.


Scientific Reports | 2017

Three dimensional printing of calcium sulfate and mesoporous bioactive glass scaffolds for improving bone regeneration in vitro and in vivo

Xin Qi; Peng Pei; Min Zhu; Xiaoyu Du; Chen Xin; Shichang Zhao; Xiaolin Li; Yufang Zhu

In the clinic, bone defects resulting from infections, trauma, surgical resection and genetic malformations remain a significant challenge. In the field of bone tissue engineering, three-dimensional (3D) scaffolds are promising for the treatment of bone defects. In this study, calcium sulfate hydrate (CSH)/mesoporous bioactive glass (MBG) scaffolds were successfully fabricated using a 3D printing technique, which had a regular and uniform square macroporous structure, high porosity and excellent apatite mineralization ability. Human bone marrow-derived mesenchymal stem cells (hBMSCs) were cultured on scaffolds to evaluate hBMSC attachment, proliferation and osteogenesis-related gene expression. Critical-sized rat calvarial defects were applied to investigate the effect of CSH/MBG scaffolds on bone regeneration in vivo. The in vitro results showed that CSH/MBG scaffolds stimulated the adhesion, proliferation, alkaline phosphatase (ALP) activity and osteogenesis-related gene expression of hBMSCs. In vivo results showed that CSH/MBG scaffolds could significantly enhance new bone formation in calvarial defects compared to CSH scaffolds. Thus 3D printed CSH/MBG scaffolds would be promising candidates for promoting bone regeneration.


ACS Applied Materials & Interfaces | 2015

Evaluation of injectable strontium-containing borate bioactive glass cement with enhanced osteogenic capacity in a critical-sized rabbit femoral condyle defect model.

Yadong Zhang; Xu Cui; Shichang Zhao; Hui Wang; Mohamed N. Rahaman; Zhongtang Liu; Wenhai Huang; Changqing Zhang

The development of a new generation of injectable bone cements that are bioactive and have enhanced osteogenic capacity for rapid osseointegration is receiving considerable interest. In this study, a novel injectable cement (designated Sr-BBG) composed of strontium-doped borate bioactive glass particles and a chitosan-based bonding phase was prepared and evaluated in vitro and in vivo. The bioactive glass provided the benefits of bioactivity, conversion to hydroxyapatite, and the ability to stimulate osteogenesis, while the chitosan provided a cohesive biocompatible and biodegradable bonding phase. The Sr-BBG cement showed the ability to set in situ (initial setting time = 11.6 ± 1.2 min) and a compressive strength of 19 ± 1 MPa. The Sr-BBG cement enhanced the proliferation and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells in vitro when compared to a similar cement (BBG) composed of chitosan-bonded borate bioactive glass particles without Sr. Microcomputed tomography and histology of critical-sized rabbit femoral condyle defects implanted with the cements showed the osteogenic capacity of the Sr-BBG cement. New bone was observed at different distances from the Sr-BBG implants within eight weeks. The bone-implant contact index was significantly higher for the Sr-BBG implant than it was for the BBG implant. Together, the results indicate that this Sr-BBG cement is a promising implant for healing irregularly shaped bone defects using minimally invasive surgery.


Journal of Materials Chemistry B | 2015

Biocompatibility and osteogenic capacity of borosilicate bioactive glass scaffolds loaded with Fe3O4 magnetic nanoparticles

Hui Wang; Shichang Zhao; Jie Zhou; Kaiping Zhu; Xu Cui; Wenhai Huang; Mohamed N. Rahaman; Changqing Zhang; Deping Wang

Multifunctional biocompatible scaffolds with enhanced osteogenic capacity coupled with magnetic and magnetothermal properties are of great interest for the repair of large bone defects resulting from the resection of tumors. In the present study, we created borosilicate bioactive glass (BG) scaffolds loaded with varying amounts (5-15 wt%) of Fe3O4 magnetic nanoparticles (MNPs) and evaluated their performance in vitro and in vivo. The incorporation of MNPs endowed scaffolds with excellent magnetic, controlled magnetothermal properties and higher mechanical capacity. The MNP-loaded scaffolds were not toxic to human bone marrow-derived stem cells (hBMSCs) cultured on the scaffolds in vitro. The alkaline phosphatase activity and the osteogenic gene expression of the hBMSCs increased with increasing amount of MNPs in the scaffolds. When implanted in rat calvarial defects for 8 weeks, the scaffolds loaded with 15 wt% MNPs showed a significantly better capacity to regenerate bone when compared to the scaffolds without the MNPs. These MNP-loaded BG scaffolds are promising implants for regenerating bone in defects resulting from tumor resection.


Journal of Materials Chemistry B | 2016

Three-dimensional printing of tricalcium silicate/mesoporous bioactive glass cement scaffolds for bone regeneration

Peng Pei; Xin Qi; Xiaoyu Du; Min Zhu; Shichang Zhao; Yufang Zhu

Bone defects, particularly large bone defects resulting from infections, trauma, surgical resection or genetic malformations, remain a significant challenge for clinicians. In this study, the tricalcium silicate/mesoporous bioactive glass (C3S/MBG) cement scaffolds were successfully fabricated for the first time by 3D printing with a curing process, which combined the hydraulicity of C3S with the excellent biological property of MBG together. The C3S/MBG scaffolds exhibited 3D interconnected macropores (∼400 μm), high porosity (∼70%), enhanced mechanical strength (>12 MPa) and excellent apatite mineralization ability. Human bone marrow-derived mesenchymal stem cells (hBMSCs) were cultured on the scaffolds to evaluate their cell responses, and the results showed that C3S/MBG scaffolds could stimulate the attachment, proliferation and differentiation of hBMSCs with increasing MBG component. The critical-sized rat calvarial defect animal model was employed; further in vivo results indicated that both C3S and C3S/MBG30 scaffolds could induce new bone formation, but the C3S/MBG30 scaffolds significantly improved the osteogenic capacity compared to the pure C3S scaffolds. Therefore, the C3S/MBG cement scaffolds fabricated by 3D printing with a curing process would be a promising candidate for bone regeneration.

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Changqing Zhang

Shanghai Jiao Tong University

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Yadong Zhang

Shanghai Jiao Tong University

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Mohamed N. Rahaman

Missouri University of Science and Technology

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Yufang Zhu

University of Shanghai for Science and Technology

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Min Zhu

University of Shanghai for Science and Technology

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Zhongtang Liu

Second Military Medical University

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Jianhua Zhang

University of Shanghai for Science and Technology

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