Shigemi Anraku

Enhancement of dopamine release from striatal slices of rats that were subchronically treated with methamphetamine.

The effect of dopamine (DA) uptake inhibitors (methamphetamine, nomifensine, and phenylethylamine) on the release of endogenous DA from striatal slices of rats pretreated with methamphetamine (6 mg/kg/day for 9 days) was investigated. The exposure of methamphetamine-pretreated rat striatal slices to a low concentration (10(-7) M, 5 X 10(-7) M) of methamphetamine caused a greater increase in DA efflux than that of saline-treated rat striatal slices. The drug-treated rats displayed an enhanced stereotyped behavioral response to a small dose of methamphetamine (1 mg/kg). Removal of Ca2+ from the superfusion medium did not affect the difference in the rates of methamphetamine (10(-7) M) induced DA release between methamphetamine-treated and saline-treated rat striatal slices. Nomifensine- and phenylethylamine-induced DA release from striatal slices was also enhanced by repeated administration of methamphetamine. On the other hand, there was no difference in K+-induced DA release between the two groups. Moreover, repeated administration of methamphetamine caused a significant increase in 3H-dopamine uptake in rat striatal synaptosomes. These results suggest that the behavioral sensitization produced by the repeated administration of methamphetamine is accompanied by an enhancement in the release of DA induced by methamphetamine, nomifensine, and phenylethylamine in vitro and is also accompanied by increased DA uptake into striatal synaptosomes.

Histochemical studies on two types of cells containing catecholamines in sympathetic ganglia of the bullfrog

The excitation/emission spectra maxima obtained from orange fluorescing small cell clusters and greenish yellow fluorescing ganglion cell bodies were at 400–410/490–560 nm and at 400–410/470–500 nm, respectively.

The acute effects of sulpiride on the central dopamine turnover in rats: A quantitative histochemical study

Sulpiride accelerated the dopamine turnover preferentially in the mesolimbic as compared to the nigrostriatal dopamine system. However, the tuberoinfundibular dopamine turnover was not affected by sulpiride or haloperidol.

Dopamine-containing cells in rabbit nodose ganglia

Microspectrofluorometry of rabbit nodose ganglia exposed to formaldehyde vapor revealed that the intraganglionic fluorescent cells (SIF-cells) contain dopamine.

A HISTOCHEMICAL STUDY ON MYOCLONUS-EPILEPSY (LAFORA-BODY TYPE)

Since Unverricht (1891) and Lundborg ( 1903) described myoclonus epilepsy as an independent illness, many reports have been made on its clinical features, but the reports of its autopsy cases are relatively few. Above all, regarding cases belonging to the “Lafora-body type” in which the intraneuronal inclusions (so-called Lafora-body or “Myoklonuskorperchen”) first described by Lafora and Cluck (191 1) are observable, only about 30 reports have been submitted so far in all the world, and in this country there are only the reports of Wakui, Tachibana, Hirai, Imai, Namba et al. to be counted. Concerning the nature of the Lafora-body there are many works by Lafora (19 1 l ) , Ostertag (1925), Buduls and Vilde (1938), Roizin and Ferraro (1942), Harriman and Millar (1955), Imai et al. ( 1956), Namba (1964), Seitelberger, Jacob, Peiffer and Colmant (1964), Gabriel and Myron (1965) and others, but their results do not always agree with each other. On the other hand, Harriman (1955), Seitelberger (1964), and Gabriel (1965) have found a substance similar to that of the Lafora-body in heart muscle and liver cells. Imai et al. (1956) were the first in this country to confirm the presence of this substance in heart muscle cells. Thus, it may be at present an accepted opinion among investigators that myoclonus-epilepsy of the Lafora-body type is probably a disease due to a carbonhydrate metabolic disturbance. Recently we have experienced one case of myoclonus-epilepsy belonging to the Lafora-body type and histochemical investigation was carried out on the Lafora-body as well as on the inclusion substance within the heart muscle and liver cell. New information obtained shzll be described here.

The short- and long-term effects of haloperidol on rat central dopamine turnover

We carried out a quantitative fluorescent histochemical analysis in rats in order to clarify the effects of chronic treatment with haloperidol on the central dopamine turnover. Long-term treatment with haloperidol showed no significant reduction of dopamine fluorescence intensities in the n. caudatus putamen, the n. accumbens, and the tuberculum olfactorium. This suggests that tolerance is established after long-term treatment with haloperidol in both mesolimbic and nigrostriatal dopamine systems, whereas tolerance is not observed in the tuberoinfundibular dopamine system.

Biochemical and Histochemical Studies on Catecholamines in Bullfrog Spinal Ganglia

Abstract: Bullfrog spinal ganglia were subjected to two types of examination to determine whether catecholamines were present. A biochemical microassay developed by Kissinger and co‐workers and a histofluorescence technique for cellular demonstration of biogenic monoamines developed earlier by Falck and co‐workers were used. Bullfrog spinal ganglia and dorsal roots were found to contain catecholamines, primarily adrenaline.

Acute and Chronic Effects of Haloperidol on Dopamine Fluorescence in the Median Eminence and on Plasma Prolactin Prolactin Levels in Rát

ABSTRACT Time course studies of the effects of haloperidol (2 mg/kg) on alpha-methyltyrosine-induced dopamine fluorescence in the median eminence and on plasma prolactin levels showed that both dopamine turnover in the median eminence and prolactin levels are significantly increased 20 h after a single i.p, injection of haloperidol. Further, hypophysectomy prevents haloperidol-induced increase in dopamine turnover in the median eminence. Moreover, a marked increase in dopamine turnover in the median eminence was found 1, 5 and 10 weeks after chronic administration of haloperidol(about 1mg/kg/day). Haloperidol administration for a week caused a significant elevation of plasma prolactin levels. Our histochemical data suggests that tolerance does not develop to the effect of haloperidol-induced prolactin on dopamine turnover in rat median eminence.

Isolation and characterization of the glycopeptide from the urine of myoclonus epilepsy of lafora-body type

Abstract Urinary glycopeptides in one myoclonus epilepsy patients of Laforabody type, one myoclonus epilepsy patient of degenerative type, and 11 normal humans were assayed by determining the carbohydrate content of materials which remained after proteolysis. Fractionation of nondialyzable glycopeptide from the patient of Lafora-body type by DEAE Sephadex A-50 column chromatography yielded three; from the patient of degenerative type, five; and from normal, five fractions, respectively; they were generally characterized by an increase in peptide and sialic acid as the molarity of Tris-HCl buffer increased. Hexose was the main component, and peptide was the next in each fraction from all the materials. The sugar components consisted of galactose, mannose, fucose, sialic acid, glucosamine, and galactosamine with an almost near content distribution in the all groups except sialic acid, which was contained three to four times more in amount in the material from the patient of Lafora-body type than from the patient of degenerative type and the normal.