Shigemi Ariyama

Lipid Analysis of helicobacter pylori

Lipids from Helicobacter pylori were extracted, isolated by conventional DEAE-Sephadex and silica gel column chromatography, and then purified by preparative thin-layer chromatography. Simple and phospholipids were analyzed by HPTLC and quantitatively determined by densitometry scanning. The fatty acid compositions of simple lipids were estimated by gas-liquid chromatography. The simple lipid composition of H. pylori consisted of wax ester (2.5%), triglycerides (4.9%), free fatty acids (30.0%), cholesterol (6.9%), diacylglycerol (29.1%), and monoacylglycerol (2.6%). The neutral phospholipid composition consisted of phosphatidyl ethanolamine (79.1%), lysophosphatidyl ethanolamine (16%), and phosphatidyl choline (1.9%). That of acidic phospholipids consisted of phosphatidic acid (52.7%) and phosphatidyl serine (47.3%). The major fatty acids of crude lipid, wax, free fatty acids, triglycerides, diacylglycerides, and monoacyl glycerides were C19:1 (cyclo), C18:2, C16:0, C19:1 (cyclo), C22h:0, and C18:0, respectively. These results are new findings and suggest significant characteristics from the metabolic, physiologic, and chemotaxonomic point of view.

A CASE OF PRIMARY ACQUIRED AGAMMAGLOBULINEMIA ACCOMPANIED WITH NODULAR LYMPHOID HYPERPLASIA OF THE GASTROINTESTINAL TRACT FROM DUODENUM TO HALF OF JEJUNUM

原発性後天性無γ-globulin血症, WHOの分類試案ではvariable common immunodeficiencyに属するとおもわれる33才の男性症例を経験したので報告する.患者は乳幼児期に麻疹,流行性耳下腺炎に罹患したが正常に回復した.とくに感冒に罹患しやすいということはなかつた. 20才頃から肺炎に反復罹患し,再三37.5°C前後の微熱を生じ,胆嚢炎にも罹患したことがある. 32才のとき無γ-globulin血症を発見された.下痢の反復など消化器症状はない.本症例は,末梢リンパ球数は正常であるが,骨髄に形質細胞はほとんど認められない.免疫グロブリンはIgG, IgA, IgM, IgD, IgEすべてにきわめて低値を示した. T cell, B cel1はほぼ正常に存し,皮内反応(PPD, Candida)は正常に反応した.消化管X線検査,内視鏡では十二指腸球部から空腸上部にかけて微細な半円形の隆起性病変をびまん性に多数みとめ,結節性リンパ過形成(nodular lymphoid hyperplasia; NLH)とおもわれた.以上のように十二指腸から空腸にかけてNLHを有する原発性後天性無γ-globulin血症の1例について報告するとともに,本邦報告例の集計を行ない報告例との比較検討をおこなつた.

Nd: YAG Laser Treatment in Early Gastric Cancer in our Institution

From December 1980 to June 1987, a total of 57 cases (63 lesions) of early gastric cancer were received laser treatment in our institution. Of them, 41 cases with 45 lesions were received follow-up examination for more than 3 months, 5 cases (5 lesions) were operated after laser irradiation, 7 cases (8 lesions) recieved follow-up less than 3 months which made impossible to evaluate therapeutic effect, and 4 cases (5 lesions) were drop-out (Table 1). The operated cases were shown in Table 2.

Abnormality of serum immunoglobulins in chronic liver disease-immune complex like substances, abnormaly basic γ-globulin, secretory IgA and anti-DNA antibody

1) Serum secretory IgA was reduced in chronic liver disease while it was increased in obstructive jaundice. 2) Serum anti-dsDNA antibody was slightly increased in chronic liver disease, especially it was significantly increased in lupoid hepatitis in which its titer paralelled with the course of disease activity. 3) Serum Clq binding activity, Clq binding inhibition activity and polyclonal rheumatoid factor binding inhibition activity were increased in chronic liver disease and their disease activity was correlated with concentration of macromolecular immune complexes which were fractionated with sucrose density gradient ultracentrifugation. 4) Abnormally basic y-globulin which was dominantly found in chronic hepatitis B sera was determined to be monomeric IgG. I t was increased in aggravation of the disease but has no correlation with Clq binding monomeric IgG. 5) Liver membrane specific lipoprotein (LP-1), Espinosas liver specific antigen (LSA), Nerenbergs hepatorenal antigen (HRA) and Tamm-Horsfall glycoprotein (THGP) had positive charge, and LP-2 and F-antigen did negative charge. 6) Human liver cell membrane fraction could not be obtained by the method of Ray or aqueous two phase polymer system which have been used for rat liver.