Shigeru Fujita
Niigata University
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Publication
Featured researches published by Shigeru Fujita.
Microbiology | 1986
Tohey Matsuyama; Tsukasa Murakami; Masashi Fujita; Shigeru Fujita; Ikuya Yano
Summary: Pigmented and non-pigmented strains of Serratia marcescens produced extracellular vesicles and had wetting activity when grown at 30°C but not at 37°C. Light microscopy showed that the red pigment was present in vesicles and intracellular granules. Electron microscopy revealed the presence of vesicles surrounded by the bacterial membrane. Three lipids having the wetting activity, W1, W2 and W3, were isolated by thin-layer chromatography of lipids from different strains of S. marcescens. Dispersions of the isolated wetting agents had small contact angles on a polystyrene surface and the ability to lower surface tension. Wetting agent W1 was the aminolipid serratamolide. Wetting agents W2 and W3 were also aminolipids but were shown to be different from serratamolide by chemical analyses. Wetting agent and prodigiosin (in a pigmented strain) were the main lipids of isolated vesicles.
Medical Mycology | 1987
Shigeru Fujita; Tohey Matsuyama
Arthrospores of Trichophyton mentagrophytes were inoculated on to the plantar part of a guinea pig foot by a newly devised non-abrasive method. Anthropophilic and zoophilic isolates required inocula of 280 and 80 arthrospores to infect 50% of inoculated feet, but much larger inocula (5 X 10(4)) were used to establish infection consistently in all feet. Anthropophilic isolate NTM-105 invaded only the upper two-thirds of the horny layer and induced no inflammatory responses. On the other hand, zoophilic isolate SM-110 invaded the whole horny layer and provoked strong inflammatory responses and clinical manifestations. Although the histological features and modes of fungal spreading in the guinea pig skin were quite different between anthropophilic and zoophilic isolate infections, infecting fungi were always recognized in the stratum corneum of all inoculated feet throughout the observation period longer than 6 months. Thus, two types of persistent infections with T. mentagrophytes were established as a guinea pig model of tinea pedis.
Mycoses | 2009
Shigeru Fujita; M. Tezuka; A. Kaise; Masaaki Ito
Summary. An excellent staining method to demonstrate in vivo dermatophytes and DNA‐synthesizing cells in the skin is described. Deparaffinized sections of guinea pig skin, infected with dermatophytes and given bromode‐oxyuridine (BrdU) by intraperitoneal injection, were used. Periodic acid—Schiff (PAS) stain in combination with an immunohistochemical stain using anti‐BrdU monoclonal antibody allowed simultaneous visualization of fungi and DNA‐synthesizing epithelial cells in the skin. Many epithelial cells near the infecting fungi in follicles and interfollicular skin were labelled with BrdU. Only a few BrdU‐positive cells were observed in the skin of uninfected animals.
Scandinavian Journal of Haematology | 2009
Kozo Nakanishi; Takaaki Hato; Atsuko Imai; Hitoshi Kaneko; Akira Murakami; Keiichi Kuwashima; Kyoichi Ikeda; Shigeru Fujita; Yuzuru Kobayashi; Hidetoshi Kaido; Masaru Kondo; Akira Hattori
Japanese Journal of Medical Mycology | 1986
Shigeru Fujita; Tohey Matsuyama; Yoshio Sato
Nippon Ishinkin Gakkai Zasshi | 1992
Shigeru Fujita
Japanese Journal of Medical Mycology | 1988
Shigeru Fujita; Tohey Matsuyama; Yoshio Sato
Journal of Antimicrobial Chemotherapy | 1997
Toshio Itoyama; Katsuhisa Uchida; Hideyo Yamaguchi; Shigeru Fujita
Nippon Ishinkin Gakkai Zasshi | 1997
Shigeru Fujita
Japanese Journal of Medical Mycology | 1989
Shigeru Fujita; Yasuyuki Kaneko; Yoshio Sato; Tohey Matsuyama