Shigeru Kiyama

Improvement of the survival rate after rat massive hepatectomy due to the reduction of apoptosis by caspase inhibitor

Background and Aim:  Acute liver failure after massive hepatectomy is caused by both necrosis and apoptosis in the remnant liver. We investigate the protective effect of the caspase inhibitor on apoptosis after massive hepatectomy in rats.

Reduction of fibrosis in a rat model of non-alcoholic steatohepatitis cirrhosis by human HGF gene transfection using electroporation.

Background and Aim:  To study the histological changes caused by transfection of the hepatocyte growth factor (HGF) gene using electroporation (EP) in a non‐alcoholic steatohepatitis (NASH) cirrhotic liver model.

Giant left-sided inguinoscrotal hernia containing the cecum and appendix (giant left-sided Amyand's hernia)

The present case involved a 62‐year‐old male with a large left‐sided inguinoscrotal hernia. A CT scan and a clinical examination led to a diagnosis of a giant left‐sided Amyands hernia. The hernia was repaired using the ULTRAPRO Hernia System (UHS), and the patient exhibited an uneventful postoperative course.

Preoperative Granulocyte-Colony Stimulating Factor (G-CSF) Treatment Improves Congested Liver Regeneration

BACKGROUND Hepatic venous congestion after liver surgery can cause liver failure. We evaluated the effectiveness of granulocyte-colony stimulating factor (G-CSF) for regeneration of remnant liver with venous congestion. MATERIALS AND METHODS Rats were divided into three groups. Group A underwent 60% hepatectomy. Group B underwent 60% hepatectomy with partial venous congestion in remnant liver. Group C underwent the same procedures as group B with G-CSF given preoperatively for 5 d. To evaluate liver regeneration in each group at 1, 2, 3, 5, and 7 d postoperatively, the proliferating cell nuclear antigen (PCNA) labeling index (LI), mitotic index (MI) in the congested area of remnant liver, and regeneration rate of remnant liver weight were measured. CD34 antibody-positive hematopoietic stem cell (HSC) colonies were identified using immunopathological staining. RESULTS Compared with group B, in group C, LI, and MI in congested remnant liver increased earlier, peak LI value in the congested area appeared 24h earlier, and full recovery of remnant liver weight occurred 2 d earlier. On postoperative day 3, remnant liver weight was significantly greater in group C than group B. On microscopy, CD34-positive cells were seen in preoperative group C livers. CONCLUSION Preoperative G-CSF improves regeneration of livers with venous congestion.